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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for Integrin alpha 4 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Molecular Biology 2020Quote: ... Digestion was performed overnight at 37 °C using recombinant dimethylated SOLu-trypsin (Sigma-Aldrich) with a trypsin:protein ratio 1:20 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Purified recombinant S proteins were concentrated using Amicon filtration devices (EMD Millipore, Billerica, MA), buffer-exchanged into PBS and analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was purified by affinity chromatography using anti-FLAG affinity resin (Sigma). The purified protein was precipitated by 20% trichloroacetic acid ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RNase R (30 pmols) or Bovine Serum Albumin (BSA, Sigma-Aldrich, MO, USA) were alone or combined with Csm5 WT or Csm5Δ46 (90 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: We concentrated recombinant EGFP fusion proteins using Amicon Ultra centrifugal filters (10K MWCO, Millipore) to an appropriate protein concentration in 250 mM NaCl salt buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µM AMP (Serva) and active recombinant AMPK (16 ng) (Sigma-Aldrich; 14-840) in a total volume of 30 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, R and D) and Purmorphamine (1.5 μM, Sigma). Plates were then incubated at 37°C at 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Developmental Biology 2023Quote: ... This basal medium was completed with 5ng/ml recombinant mouse LIF (Sigma ref LIF2050), 3µM CHIR99021 (Sigma ref SML1046-5MG ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Neuroscience 2021Quote: ... washed twice in PBS and incubated overnight at 4°C in primary anti-KCC2 raised in rabbit (1:1000, Millipore/Upstate, Cat. #07–432), anti-CGRP raised in mouse (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunoprecipitated overnight at 4 °C with 2 µg affinity-purified rabbit anti-PKM2 antibody raised against a C-terminal peptide (Sigma cat. # SAB4200105, Lot #030M4874) pre-coupled to Dynabeads sheep M-280 anti-rabbit IgG beads (LifeTech) ...
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bioRxiv - Pathology 2020Quote: ... and 0.1% Triton-X 100 in PBS) and stained overnight at 4° C with antibodies against PRG2 (1:1000 dilution, rabbit; Sigma-Aldrich cat. no. HPA038515), HLA-G (1:10,000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... The rest of the pre-cleared lysates was then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at a 1:400 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... the tamoxifen active metabolite 4-hydroxytamoxifen (4-OHT, Sigma, cat#H6278) was used instead of tamoxifen ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently fixed overnight at 4°C in 4% paraformaldehyde (Sigma). Next ...
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bioRxiv - Biophysics 2021Quote: ... 4 cm by 4 cm in chloroform (Sigma Aldrich, analytical standard) and placed the piece of paper inside the plastic container with the recorded fungal substrate.
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 1μM 4-hydroxy tamoxifen (4-OHT) (Sigma) for 48h ...
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bioRxiv - Neuroscience 2020Quote: ... QX314 chloride and 4-aminopyridine (4-AP) were from Sigma-Aldrich, (St ...
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bioRxiv - Biophysics 2019Quote: ... 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (4-hydroxy-TEMPO, Sigma-Aldrich) was added at a concentration of 0.05% (w/w ...
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bioRxiv - Immunology 2019Quote: ... the inhibitor 6-amino-4–4-phenoxyphenylethylamino-quinazoline (Merck Millipore, USA) was reconstituted in DMSO at a stock concentration of 1mM and administered at a final concentration of 10nM on Days 0 and 4 ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were induced using 4-Hydroxytamoxifen (4-OHT) (H7904; Sigma H7904) from fresh or pre-heated (65°C for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Shield-1 (Cheminpharma LLC) and 4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich) at 1μM were added for one hour to allow TRF1 to enter the nucleus prior to live imaging or two hours prior to fixing ...
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bioRxiv - Microbiology 2022Quote: ... 3 days post-infection 4 mL of paraformaldehyde 4% (#158127, Sigma) was added and incubated overnight at 4ºC ...
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bioRxiv - Immunology 2023Quote: ... 100 μM 4-aminobenzoic acid hydrazide (4-ABAH, Sigma-Aldrich, A41909) or 20 μM disulfiram (Tocris ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were fixed overnight at 4°C in 4% paraformaldehyde (Sigma) in PBST ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were fixed overnight at 4°C in 4% paraformaldehyde (Sigma) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µM or 500 nM 4-Hydroxytamoxifen (4-OHT; Sigma # H7904) for 4 hours to induce Fok1- or AsiSI-dependent DSB ...
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bioRxiv - Molecular Biology 2023Quote: ... 4-Hydroxytamoxifen (4-OHT) and Etoposide were obtained from Sigma Aldrich, BafilomycinA1 was purchased from Cambridge Bioscience and BrDU was purchased from Abcam.
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bioRxiv - Plant Biology 2024Quote: ... through quantification of the fluorescence of 4-MU (4-Methylumbelliferone, Sigma) reaction product ...
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bioRxiv - Molecular Biology 2024Quote: ... for 2 h or 4-hydroxytamoxifen (4-OHT, Sigma, 10 µM) for 4 h ...
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bioRxiv - Biochemistry 2024Quote: ... cells were treated with 1 μM 4-hydroxytamoxifen (4-OHT) (Sigma) and 1 μM Shield1 (Clonetech Labs ...
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bioRxiv - Cell Biology 2023Quote: ... Normal Rabbit IgG (12-370, Sigma-aldrich, Rabbit polyclonal), Normal Mouse IgG (12-371 ...
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bioRxiv - Genetics 2019Quote: ... Expression of Scc1(R180D,268D)-HA was induced 1h after starting alpha-factor treatment by addition of newly-made galactose (Sigma-Aldrich, G0750) (2% final ...
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bioRxiv - Biochemistry 2020Quote: ... The material was transferred into no-stick microtubes (Alpha Laboratories, cat. LW2410AS) and supplemented with 150 U/ml of Benzonase nuclease (Millipore, cat. 71206). Samples were incubated on a rotating wheel for 30 min at 4°C and centrifuged (16,000g ...
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bioRxiv - Cell Biology 2022Quote: ... Alpha smooth-muscle actin (αSMA) and collagen I (col1) were stained using monoclonal antibodies (1:1000 diluted, Sigma, A2547 and SAB4200678, respectively). For detection of primary antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... Both vectors are Gateway® compatible and instructions outlined by Gateway® Technology were followed to complete the LR reaction and transformed in SIG10-5 alpha chemically competent cells (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were then cut based on size and incubated overnight with the following primary antibodies: 1 μg/mL mouse α-alpha-tubulin (EMD Millipore), 1 μg/mL mouse mAb414 (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were activated with a cocktail of T-cell activators consisting of 40 ng/ml tumor necrosis factor-alpha (TNF-α; Catalog no. T0157; Sigma-Aldrich), 40 ng/ml phorbol myristate acetate (PMA ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Immunohistochemical (IHC) staining for smooth muscle actin was carried out using anti alpha-Smooth Muscle-Cy3 monoclonal antibody C6198 (Sigma-Aldrich Co.).