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Citations for Millipore Sigma :
1401 - 1450 of 3870 citations for Human IL 17 Receptor since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and endotoxin-free recombinant human Albumin (Sigma A9731) at a final concentration of 5 mg/mL ...
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bioRxiv - Immunology 2024Quote: ... and 5% human heat-inactivated AB serum (Sigma). The antibodies used for stimulation were anti-human CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2024Quote: ... an added positive human IgG Kappa (Sigma-Aldrich) loaded control lane was included (lane P) ...
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bioRxiv - Molecular Biology 2020Quote: ... but with a 10% (w/v) glucose solution containing a yellow dye (1 mg mL−1 Acid Yellow 17; Sigma-Aldrich, St Louis, Missouri). This procedure was repeated during 8 consecutive days with the papers changed every day and blue and yellow dyes on cottons switched between days.
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bioRxiv - Microbiology 2022Quote: ... hormones were maintained in the culture medium throughout the course of each assay. 17-ß-estradiol (i. e., E2) and progesterone (i. e., P4) (both from Sigma; St. Louis, MO) were selected for use in infection studies because these hormones represent the major forms of estrogens and of progestogens (respectively ...
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bioRxiv - Cell Biology 2022Quote: Cannulated lungs from 17-18-month-old male C57BL/6 mice were filled with warm low melting point agarose (3%, Sigma-Aldrich, St. Louis, MO). Lung segments were cooled on ice for 30 min to allow solidification of the agarose ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Dexamethasone (DEX, cat. #D4902), prednisolone (PRED, cat. #P6004), and medroxyprogesterone 17-acetate (MPA, cat. #M1629) were all purchased from Sigma-Aldrich (St. Louis, MO).
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bioRxiv - Zoology 2021Quote: ... Wayne, NJ, USA; 800 frames per second, 1000 μs exposure; Lenses: Sigma DC 17- 50mm 1:28 EX HSM, SIGMA Corporation, Ronkonkoma, NY, USA) and three LED lights (Veritas Constellation 120 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleaved product was loaded onto a Ni column (Cytvia HisTrap™ High Performance; Catalogue #: GHC-17-5247-01; Sigma-Aldrich, St. Louis, MO) and washed with buffer A (200 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... the synaptic recycling vesicles were loaded with 10 μM FM1-43 in a high-potassium (45 mM) extracellular solution containing the NMDA receptor antagonist (2R)-amino-5-phosphonovaleric acid (APV, 25 μM, Sigma-Aldrich, St Louis, MO, USA) and the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX ...
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bioRxiv - Neuroscience 2019Quote: The following drugs were used for behavioral pharmacology experiments: the non-selective β-adrenergic receptor (βAR) antagonist DL-propranolol hydrochloride (Sigma-Aldrich, St. Louis, MO), the α1AR antagonist prazosin hydrochloride (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... we dissolved 10 μM FM1-43FX in a high potassium (45 mM) extracellular solution containing the NMDA receptor inhibitor (2R)-amino-5-phosphonovaleric acid (APV, 25 µM, Sigma-Aldrich, St Louis, MO, USA) and the AMPA receptor inhibitor 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX ...
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bioRxiv - Microbiology 2021Quote: ... IL-22) were determined using the Mouse Th17 Magnetic Bead Panel Milliplex MAP kit (Millipore). Samples were not diluted and assay was performed according to the manufacturer’ instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... IL-6 and TNFα using Milliplex Map Mouse Serum Adipokine Panel (Millipore catalogue # MADKMAG-71K) on a Luminex 200IS platform.
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bioRxiv - Immunology 2022Quote: ... Release of IL-2 into supernatant was measured using a commercial ELISA kit (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... For IFN-γ and IL-17A expression cells were first stimulated with1 μM ionomycin (Sigma) and 20 ng/ml PMA (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 15 ng/mL IL-34 and 20 ng/mL bFGF (Sigma, Saint Louis, Missouri, USA). The culture media was changed every 4 days until cell cultures reached full confluence ...
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bioRxiv - Immunology 2023Quote: Islet MTs were treated with IL-1β (2, 5 or 10 ng/mL, Sigma-Aldrich), IFNγ (10 ...
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bioRxiv - Immunology 2023Quote: ... and IL-5 cultured cells were pulsed (4 hr) with BrdU (10 µM) (Sigma-Aldrich) prior to flow staining with an anti-BrdU-Alexa fluor 647-conjugated antibody (BD Pharminogen ...
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bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Pathology 2023Quote: ... adiponectin levels were measured by enzyme-linked immunosorbent assays (Human Chemerin ELISA kit, Biovendor, Brno, Czech Republic; Human Resistin ELISA kit, Millipore Sigma; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
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bioRxiv - Cancer Biology 2022Quote: ... both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060)) in parallel ...
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bioRxiv - Bioengineering 2020Quote: ... The O/N culture was back-diluted 1:300 in 3 mL fresh Cal18-2 medium85 (Glucidex 12 was exchanged for Maltodextrin DE 13-17 (Sigma-Aldrich, Saint Louis, MO, USA) supplemented with chloramphenicol also in an Axygen 24-deep well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysate was then added to 1 mL of a Ni bead slurry (Ni Sepharose® High Performance; Catalogue #: 17-5268-02; Sigma-Aldrich, St. Louis, MO) and incubated overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 1.25 mg/ml human insulin (Sigma #I9278), 1 % Penicillin-Streptomycin (Sigma #P4333 ...
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bioRxiv - Neuroscience 2022Quote: ... human basic fibroblast growth factor (bFGF) (Sigma, cat. #F0291), HEPES 1M (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 ng/mL of human bFGF (Sigma-Aldrich). Cells were incubated at 37 °C in a humidified atmosphere with 5% of CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Normal Human Dermal Fibroblasts (NHDFs) (Sigma-Alrdrich, Buchs, Switzerland), a non-cancerous human skin fibroblast cell line ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10 % heat-inactivated pooled human serum (Sigma) and 100 ng/ml macrophage colony stimulating factor (R&D systems) ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgG (Fab specific) – peroxidase-conjugated antibody (Sigma) diluted 1:5000 in blocking buffer was added to the wells and incubated for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... human Collagen IV from Sigma-Aldrich (St. Louis, MO), recombinant human Tenascin C ...
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bioRxiv - Cell Biology 2020Quote: ... or shRNA directed against human IP6K1 (TRC0000013508, Sigma-Aldrich) were co-transfected with VSV-G and psPAX2 (a gift from Didier Trono ...
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bioRxiv - Biochemistry 2019Quote: ... 1 µg human GST-Rb (48-378 Sigma Aldrich) as substrate and 0.2 mM ATP ...