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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for Human High Sensitive Leucine Rich Alpha 2 Glycoprotein 1 LRG1 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Alpha-tomatine was purchased from Extrasynthese (Genay, France) and tomatidine from Sigma Aldrich (St. Louis, MO, USA).
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bioRxiv - Microbiology 2024Quote: ... thermophila and alpha carbonic anhydrase from bovine erythrocytes were purchased from Sigma-Aldrich (St Louis, MO, USA). Absolute methanol was of analytical grade purchased from Thermo Fisher Scientific Inc ...
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bioRxiv - Molecular Biology 2024Quote: ... maintained in high glucose DMEM (Fisher cat #11-965-118) supplemented with 10% FBS and 2 μg/mL doxycycline (Sigma cat#D9891). The day before transfection RPLP cells were plated into two 15cm dishes using DOX-free media (+10% FBS ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM CaCl2 and 10 mM HEPES at pH 7.4 with 1-2 mM qBBr (Sigma-Aldrich or Toronto Research Chemicals ...
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bioRxiv - Microbiology 2022Quote: ... and 2 µg/ml L-1-Tosylamide-2-phenylethyl-chloromethyl-ketone (TPCK)-treated trypsin (Sigma-Aldrich, USA). Viral infectivity titers were measured in MDCK-II cells in 96-well plates ...
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bioRxiv - Microbiology 2019Quote: ... and alternative NADH dehydrogenase inhibitor flavone (2-Phenyl-4H-1-benzopyran-4-one, 2-Phenylchromone, Sigma-Aldrich) (Martins et al ...
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bioRxiv - Neuroscience 2020Quote: ... protease inhibitor cocktail tablets and 2 mg/mL pepstatin A dissolved in ethanol:dimethyl sulfoxide 2:1 (Sigma). To minimize inter-blot variability ...
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bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
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bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were resuspended in 200µl of LCMS-grade acetonitrile/methanol/water (2:2:1) (Sigma Aldrich) chilled to -20°C and transferred to lysing matrix Y tubes (MP Biomedicals ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ml ion exchange cartridge packed with 100 mg of 2– 2(pyridyl)ethyl silica gel (Sigma) was activated with 1 ml of methanol and equilibrated with 1 ml of buffer A (methanol/H2O 1:1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (33-35) and selected with 2 µg/mL puromycin (Sigma-Aldrich). To vary glucose concentration ...
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bioRxiv - Microbiology 2021Quote: Samples were diluted 1:2 in serum matrix for analysis with Milliplex Human Cytokine/Chemokine Magnetic Bead Panel as per manufacturer’s instructions (EMD Millipore Corporation). Concentrations for analytes (EGF ...
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bioRxiv - Microbiology 2021Quote: ... 293T-ACE2 cells stably expressing human ACE2 are derived from 293T cells and were maintained in medium supplemented with 2 µg/mL of puromycin (Millipore Sigma) (Prévost et al. ...
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bioRxiv - Cell Biology 2021Quote: HUVEC were seeded at 5×104 cells or 2×105 cells in 24-well plates on coverslips coated with 10 μg/ml fibronectin (from human plasma, Sigma Aldrich) and incubated overnight in complete EBM-2 medium ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (20) and selected with 2 μg/mL puromycin (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Cancer Biology 2020Quote: ... All other cell lines were grown in Dulbecco`s modified Eagle medium (DMEM) (Fisher, UK) supplemented with 2% human serum (Sigma, UK) and maintained in an humidified sterile incubator with 5% CO2 at 37°C.
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CHO cells were stably transfected with a SNAP-tagged human D2SR (short isoform) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 2 mM glutamine (Sigma-Aldrich, Poole, UK) and supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.75 IU/kg of human insulin (Actrapid 100 IU/mL, Novo Nordisk) for insulin tolerance tests (ITT) or 2 g/kg pyruvate (Sigma-Aldrich) for pyruvate tolerance tests (PTT ...
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bioRxiv - Systems Biology 2024Quote: The MIA PaCa-2 pancreatic cancer and HEK 293 human embryonic kidney were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma Aldrich, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... This was after 2 washes in Buffer A (Sigma DUOlink PLA kit) and then incubated with the PLA kit Ligation solution (1X ligation buffer ...
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bioRxiv - Microbiology 2024Quote: ... at different concentration (1:1, 1:2, 1:10), 0.05% Tween-20 (BP337-100, Fisher BioReagents™) or 1% BSA (A6003, Sigma-Aldrich), was added on top of the concentrated spore suspension ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was reverse transcribed to cDNA with the Transcriptor High Fidelity cDNA Synthesis Kit (#5081963001, Millipore Sigma) using the manufacturers protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was reverse transcribed to cDNA using the Transcriptor High Fidelity cDNA Synthesis Kit (#5081963001, Millipore Sigma) and stored at -20°C until analysis ...
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bioRxiv - Neuroscience 2023Quote: Mouse High Sensitivity T Cell Magnetic Bead kits were purchased from EMD Millipore (MHSTCMAG-70KPMX, Burlington, MA, USA) and used to quantify 18 different mouse cytokines in accordance with the manufacturer’s recommendations (belonging to 4 main categories ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... mouse ascites MAb B-5-1-2 α-tubulin 1:200 (Sigma-Aldrich #T5168); rat TgEB1 1:100 (Chen and Gubbels ...
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bioRxiv - Neuroscience 2019Quote: Complete Freund’s Adjuvant (CFA, 20 µl, 2% in 1:1 saline) (Sigma-Aldrich, F5881) was injected into the hindpaw of mice ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-α-tubulin 1:10,000 (monoclonal B-5-1-2, Sigma-Aldrich). To image the blot the secondary antibodies goat anti-rabbit IgG IRDye 800CW (LI-COR ...
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bioRxiv - Developmental Biology 2022Quote: ... and diphospho-ERK-1 and ERK-2 (1:4000; clone MAPK-YT; Sigma; RRID:AB_477245). In our hands ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were picked up in a 1:1 mixture of 2% methylcellulose (Sigma-Aldrich) and 2.3 M sucrose ...
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bioRxiv - Physiology 2019Quote: ... 1% penicillin-streptomycin and 2 μg.ml−1 puromycin dihydrochloride (Sigma, St. Louis, MO, USA) were added the next day ...
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bioRxiv - Plant Biology 2021Quote: ... 2 g L-1 Phytagel and 1.5 g L-1 activated charcoal (Sigma-Aldrich). After inoculation ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). For tissue samples ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was mixed 1:1 with RIPA buffer with 2% sarkosyl (Sigma L9150) and incubated for 1 hour at room temperature with shaking ...
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bioRxiv - Microbiology 2022Quote: ... and then 2 ml overlay comprising 1:1 viral growth medium: 2.4% cellulose (Sigma) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek) ...
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bioRxiv - Developmental Biology 2023Quote: ... counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma, 1:2000 in 1× PBS) for 5’ and mounted with fluorescent mounting medium (Dako) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), MCM2 (Abcam ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... and against α-tubulin (clone B-5-1-2) (#T5168; 1:10.000; Sigma-Aldrich). Polyclonal antibodies in this study were used against USP28 (#HPA006778 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM MgCl2 and 1 mM EGTA) containing 2 µM unlabeled phalloidin (Sigma, #P2141) and 10 µM taxol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The cells were overlaid with 1:1 volume of 2 % methyl cellulose (Sigma, USA) in 2X MEM supplemented with 5 % FBS and plate was incubated for 48 h ...
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bioRxiv - Microbiology 2020Quote: ... the Milliplex® Map Non-Human Primate Cytokine Magnetic Bead Panel Kit (EMD Millipore Corporation, Billerica, MA) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Albumin was measured in the cell culture medium by Human ALB/Serum albumin ELISA Kit (Sigma-Aldrich) and in the cell lysate by qPCR ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...