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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for 7 Chloro 1H pyrrolo 3 2 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... tendon fascicles (n=6, independent samples from different mice) were stained with 2’,7’-Dichlorofluorescin diacetate (DCFH-DA, D6883, Sigma Aldrich). Control fascicles and cultured fascicles were incubated for 30 minutes with 50μM DCFH-DA in PBS at either 29°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then washed with 100 μL of PBS and incubated with 100 μL of 20 μM 2’,7’-dichlorofluorescin diacetate (DCFH-DA, Sigma D6883) in DMEM in the dark for 40 min at 37 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... a freshly prepared solution of 5-(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; Sigma-Aldrich Co., St. Louis, MO, USA) in DMSO ...
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bioRxiv - Microbiology 2022Quote: ... and 100 μl spread on either LB agar plates with or without specified antibiotics (2 μg/ml amikacin disulfate salt or 7 μg/ml nitrofurantoin (both Sigma-Aldrich) or 50% (v/v ...
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bioRxiv - Developmental Biology 2022Quote: ... (1 mg/ml) and incubated in 50 μl droplets of 10 µM 2′,7′- dichlorodihydro-fluorescein diacetate (H2DCFDA, D6883, Sigma-Aldrich) in PVA-PBS for 15 min at 37 °C in an atmosphere of 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... CD14+ macrophages differentiated for 7 days in M-CSF (described above) were detached from the petri dish using 2 mM ethylenediaminetetraacetic acid (EDTA, Sigma Aldrich) in PBS at 4 °C as well as mechanical scraping ...
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bioRxiv - Plant Biology 2019Quote: ... wild and transgenic seeds were grown on 0.8% agar-solidified 1/2 MS vertically oriented plates for 7 days with or without specific concentration of Propyzamide (Sigma-Aldrich). To detect the morphology of the cells of 7-day-old seedlings ...
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bioRxiv - Plant Biology 2020Quote: Accumulation of ROS was assayed by confocal microscopy of seedlings stained with the fluorescent dye 2’,7’-dichlorofluorescin diacetate (H2DCFDA; Sigma-Aldrich) as described previously (Achard et al. ...
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bioRxiv - Plant Biology 2024Quote: Flowers at position 6 were gently incubated in staining solution (25 µM (2’,7’-dichlorodihydrofluorescein diacetate) (H2DCF-DA) (Sigma-Aldrich, D6883), 50 mM KCL ...
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bioRxiv - Microbiology 2023Quote: ROS production within protozoan food vacuoles was investigated by staining cells with the ROS indicator 2’,7’-Dichlorofluorescein diacetate (DCFH-DA, Sigma Aldrich). After deacetylation by cellular esterases ...
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bioRxiv - Cell Biology 2023Quote: LPS- and OGD-treated cells were washed with PBS and stained with 5 μM 2′,7′-dichlorofluorescin diacetate (DCFH-DA; Sigma-Aldrich) for 20 min at 37°C to detect cell reactive oxygen species (ROS) ...
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bioRxiv - Bioengineering 2023Quote: ... the surfaces of interest were incubated with a sterile filtered solution of 2 mg/mL dopamine (Dopamine 62-31-7, Sigma Aldrich) in 10 mM Tris-HCl (pH 8.5 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: We used the odorants methyl hexanoate (MHXE, CAS: 106-70-7) and 2-heptanone (HEPN, CAS: 110-43-0) (Sigma Aldrich). Odorant stimuli were generated with a custom-made stimulator optimized for temporal precision in binary mixtures [13] ...
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bioRxiv - Systems Biology 2022Quote: ... The dried extracts were dissolved in 15 μL or 20 μL of methoxyamine hydrochloride solution (40 mg/mL in pyridine, both Sigma Aldrich, St. Louis, Missouri, U) and incubated for 90 min at 30 °C with constant shaking ...
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bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Three subsamples (∼2 ml) were taken from each section with a 3 ml syringe and immediately fixed in 2 ml RNAlater (Sigma Life Science) in a 5 ml tube (Qiagen ...
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bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Biochemistry 2022Quote: ... were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt, Aldrich) or negatively charged 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS, Sigma-Aldrich, Inc) containing N,N’-methylenebisacrylamide (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were blocked in non-fat milk with 0,1% Tween 20 for 1h (Sigma-Aldrich), and incubated with antibodies as indicated in Table S2 at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... and then primed for 1h at room temperature with 1% Bovine Serum Albumin (BSA, Sigma) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were permeabilized and blocked for 1h at room temperature in 0.3% Triton (Sigma-Aldrich)/5% goat serum (Jackson Laboratories) ...
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bioRxiv - Genomics 2019Quote: ... all embryos were cultured for 1h in KSOM+AA medium (Cat. MR-121-D, Millipore) at 37°C under 5% CO2 in air before being transferred into 7-10 week-old female CD1 recipients.
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bioRxiv - Neuroscience 2021Quote: ... Blocking was performed for 1h at RT using 20% normal goat serum (S26, Sigma-Aldrich) in Tris-NaCl blocking buffer (TNB) ...
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bioRxiv - Biochemistry 2020Quote: ... 0.625 mM TBTA (Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine) (Merck Millipore), and 6.25 mM CuSO4 (Merck Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 1h in base assay medium (D5030, Sigma Aldrich, Merck, Darmstadt, Germany) supplemented with 2 mM glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... 400 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (678937, Sigma, UK) and 8 mM ascorbic acid (A15613 ...
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bioRxiv - Developmental Biology 2021Quote: ... except that embryos were incubated for 1h in pre-equilibrated KSOM (without amino acids; Millipore), prior to incubation in KSOM containing 500 μm HPG or 1 mM EU for 2h ...
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bioRxiv - Biophysics 2020Quote: ... The purified protein was incubated and for 1h at 37°C with bovine thrombin (Sigma) in a 1000:1 molar ratio ...
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bioRxiv - Cell Biology 2020Quote: ... 400 μM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (678937, Sigma, UK) and 8 mM ascorbic acid (A15613 ...
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bioRxiv - Microbiology 2023Quote: ... Co-immunoprecipitated proteins were eluted for 1h at 4°C with 3X FLAG peptide (Sigma) in TBS (pH 7.4) ...
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bioRxiv - Immunology 2024Quote: ... and incubated for 1h at RT with 1% BSA + 0.3% TritonX-100 (Sigma Aldrich T9284) in DPBS ...
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bioRxiv - Immunology 2023Quote: ... MDMs were also pre-treated for 1h with a TLR3/dsRNA complex inhibitor (Merck Millipore) 5 µM final concentration ...
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bioRxiv - Cell Biology 2023Quote: ... The collected epidermis and dermis were pre-fixed 1h in 4% paraformaldehyde (PFA, Sigma-Aldrich), rinsed three times with PBS for 5 min and conserved in PBS with 0.2% azide at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... samples were incubated for 1h in PBS supplemented with 0.3% Triton X-100 (Sigma-Aldrich), 1% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... Where indicated iAstrocytes were pre-incubated for 1h with 0.5 µM Avasimibe (PZ0190, Sigma Aldrich), 10µM Cholesterol (C4951 ...
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bioRxiv - Immunology 2020Quote: ... Wapl-degron v-Abl pro-B lines were treated with 150 μM Indole-3-acetic acid (auxin analog, IAA, Sigma-Aldrich, #I3750-25G-A) and 2 mg/mL doxycycline (Dox ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Cell Biology 2022Quote: ... Log phase promastigote cultures (seeded at 2×106 cell/ml) were allowed to grow in complete M199 supplemented with 150 μM biotin (B-4639, Sigma-Aldrich) for 18-24 hours ...
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bioRxiv - Biophysics 2021Quote: ... Au-micellar solution was obtained first by dissolving a diblock copolymer polystyrene-b-poly(2-vinylpyridine) (Polymer Source) in o–xylene and second by loading with HAuCl4 · 3H2O (Sigma Aldrich) according to the specific parameter L = n[HAuCl4]/n[P2VP] ...
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bioRxiv - Cell Biology 2021Quote: ... The tissue was homogenized 20 times with pestle A and 20 times with pestle B in 2 ml of ice-cold nuclei EZ lysis buffer (NUC101-1KT, Sigma-Aldrich). Then ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... half of the medium was substituted with a culture medium supplemented with 2% B-27 plus supplement and 5μM of cytosine β-d-arabinofuranoside (Ara-C; Sigma; #C1768). The culture medium was then changed every three days ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Immunology 2021Quote: ... Latrunculin B (Lat B, 428020, MilliporeSigma) and Jasplakinolide (Jas, 420107, MilliporeSigma)/Blebbistatin (Bleb, B0560, Sigma-Aldrich) were used for manipulating CD20 mobility ...
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bioRxiv - Bioengineering 2021Quote: A 1.4wt% agarose hydrogel containing 100 mg/mL (C0) Rhodamine B (Rho B) (Sigma Aldrich, R6626) was prepared ...
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bioRxiv - Immunology 2021Quote: ... resting B cells were pre-cultured in B cell culture medium with 20 μM DAPT (Sigma) or with vehicle (DMSO ...
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bioRxiv - Immunology 2021Quote: The livers of freshly-sacrificed mice were perfused retrogradely via the IVC(3) with 3 ml of PBS and then 10 ml of 2% paraformaldehyde (Sigma, catalogue# 30525-89-4) in PBS ...