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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for 2S 4S 4 Fmoc amino pyrrolidine 2 carboxylic acid methylester hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A 0.8% (w/v) aqueous solution of 2-thiobarbituric acid (TBA, Sigma) was prepared by heating the solution to 50°C for 2h ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μg/ml Vitamin C (L-Ascorbic acid 2-phosphate, Sigma A8950), 1% GlutaMAX (ThermoFisher 35050061 ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 μg/ml Vitamin C (L-Ascorbic acid 2-phosphate, Sigma A8950), 20 ng/ml recombinant human LIF (Peprotech 300-05) ...
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bioRxiv - Cell Biology 2022Quote: ... 2-mercaptoethanesulfonic acid (MESNA) and iodoacetamide from Sigma Aldrich (63707 and I1149), the Cdc42 inhibitor ML141 from Tocris Bioscience ...
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bioRxiv - Neuroscience 2020Quote: ... propionic acid and 2-butanone (these four odorants purchased from Sigma-Aldrich), and female stonefly ...
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bioRxiv - Neuroscience 2021Quote: ... 50 µg/ml L-Ascorbic acid 2-phosphate sesqimagnesium salt hydrate (Sigma), 10 ng/ml Heregulin β (Peprotech) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 μg/mL L-Ascorbic acid 2-phosphate (Cat# A8960, Sigma). The induced cells underwent half media replacement every 2-3 days until harvest.
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bioRxiv - Microbiology 2022Quote: ... and 20 μg/mL 1,4-dihydroxy-2-naphthoic acid (DHNA, Sigma-Aldrich). Culture medium was supplemented with 10 μg/mL erythromycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... and 50 ug/mL of L-Ascorbic acid 2-phosphate (Sigma-Aldrich) for a total of 28 d ...
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bioRxiv - Cell Biology 2024Quote: ... in buffer (100mM 2-(N-Morpholino)ethanesulfonic acid hydrate (M8250; Sigma-Aldrich), 1mM trans-1,2-Diaminocyclohexane-N,N,Nʹ,Nʹ-tetraacetic acid monohydrate ...
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bioRxiv - Plant Biology 2023Quote: ... or 5 mM ethephon (2-chloroethanephosphonic acid, an ethylene-releasing agent, Sigma). Both MeJA and ethephon were prepared with distilled water ...
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bioRxiv - Immunology 2024Quote: ... 30 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich), 1% Glutamax (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... + 50 µg/mL Ascorbic Acid-2 Phosphate (A8960-5G, Sigma-Aldrich Ltd.) + 10 ng/mL TGF-β (SRP3171 ...
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bioRxiv - Genomics 2024Quote: ... in 100 mM 2-(N-morpholino)ethanesulfonic acid (MES; Sigma-Aldrich #M1317) buffer (pH 6.5 ...
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bioRxiv - Microbiology 2022Quote: ... membranes were incubated for 1 min with a 1:1 mix of solution A (2.5 mM luminol [Sigma], 0.4 mM p-coumaric acid [Sigma], 100 mM Tris-HCl, pH 8.5) and solution B (0.018 % H2O2 ...
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bioRxiv - Microbiology 2022Quote: ... were place on the plate and 10 μL of (1, 2, 4 and 9.8 M) H2O2 and (2, 4, 6 M) methyl viologen (Sigma-Aldrich, UK) was dispensed onto each separate disk ...
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bioRxiv - Bioengineering 2022Quote: 4-Nitrophenyl β-D-glucuronide (4-NPG, CAS no. 10344-94-2) (Sigma-aldrich) was prepared in 50 mM sodium phosphate buffer (Na-PB ...
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bioRxiv - Biochemistry 2023Quote: ... and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride (Sigma-Aldrich) in buffer C for 1 h at 37°C and 500 rpm using a ThermoMixer (Eppendorf) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Marchantia 5-7 day old sporelings were co-cultivated with 100 µL of Agrobacterium culture in 4 mL of liquid 0.5x Gamborg B5 plus supplements (0.1% N-Z amino A (Sigma C7290), 0.03% L-glutamine ...
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bioRxiv - Cell Biology 2021Quote: TPP1 enzyme activity was measured fluorometrically based on fluorescence of the chromophore 7-amino-4-methylcoumarin (AMC) (Sigma #A9891) as described previously (Lojewski et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Spores were grown on solid 0.5× Gamborg B-5 media for 5 days and dispensed in 6-well plates containing 4 mL of liquid 0.5× Gamborg B-5 plus supplements: 0.1% N-Z amino A (Sigma #C7290) 0.03% (w/v ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 2 mM MgCl2 and protease inhibitor cocktail [Sigma]). 10% of lysate volume was reserved to determine total protein amounts ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested in TENT buffer (50 mM Tris-HCl pH 8.0, 2 mM EDTA pH 8.0, 150 mM NaCl, 1% Triton X-100; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Bioengineering 2020Quote: ... Recovered samples were resuspended in 50 mM tris-HCl (pH 8.0) and mixed with equal volumes of 2 mM monobromobimane (mBBr; Sigma). After 15 minutes of incubation in mBBr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated in sucrose buffer (0.1 M Tris-HCl pH = 7.5, 2 mM MgCl2, 1 M sucrose, 1 % Protease Inhibitor Cocktail (Sigma Aldrich), 200 μg.mL−1 RNase A and 10 μg.mL−1 DNase (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: AuMA NPs were synthesized by covalently-linking AuCOOH NPs with 2-aminoethyl methacrylate (AEMA, 90%, C6H11NO2·HCl, Sigma-Aldrich) using carbodiimide/succinimide chemistry (Fig ...
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bioRxiv - Biophysics 2022Quote: ... permeabilized with 0.2% Triton X-100 in TBS (50 mM Tris-HCl, 0.15 M NaCl, pH 7.4) and blocked with 2% BSA (Sigma Aldrich) in TBS ...
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bioRxiv - Immunology 2020Quote: ... each of the anti-MuSK clones was combined with 1.3-1.4x molar excess of the b12 antibody and 75 mM 2-Mercaptoethylamine-HCl (Sigma-Aldrich) for five hours at 31°C (Labrijn et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was suspended in buffer solution (20u Tris-HCl pH 7.5, 2u u mM 2-mercaptoethanol) with protease inhibitor cocktail (Sigma), 10 μg/mL aprotinin ...
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bioRxiv - Microbiology 2024Quote: ... from the resin was immediately neutralized with 1/10 faction volume of 1 M Tris-HCl pH 8.5 and concentrated using an Amicon Ultra-2 Centrifugal Filter Unit 100kDa NWMCO (Merck Millipore), in which the elution buffer was exchanged with PBS (GIBCO).
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bioRxiv - Developmental Biology 2024Quote: ... The protein sample was then diluted to appropriate concentration in SDS loading buffer (50 mM Tris-HCL pH6.8, 2% SDS (Sigma), 10% glycerol (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: Tumor tissues were snap frozen upon harvest and homogenized in lysis buffer (Tris-HCl pH 6.8, 62.5 mM; SDS, 2%; protease inhibitor cocktail (Sigma #11873580001); phosphatase inhibitors (Sigma #4906845001) ...
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bioRxiv - Immunology 2023Quote: ... RIPA 400 mM NaCl, (3) Sarkosyl Buffer (2 mM EDTA, 50 mM Tris-HCl, 0.2% sarkosyl (Lauroylsarcosine sodium salt (Sigma)) ...
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bioRxiv - Biochemistry 2023Quote: ... Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2, Merck; adjusted to pH 7 with HCl; 0.1% Tween 20, Sigma) overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 100 μL of lysis buffer (2% SDS, 60 mM DTT in 40 mM Tris HCl pH=9.0 containing protease inhibitor cocktail, purchased from Sigma). Then 20 μL of Laemmli 6X (final concentration 1x ...
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bioRxiv - Molecular Biology 2024Quote: ... A 3 ml sucrose cushion (10 mM Tris-HCl pH 7.5, 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 µg/ml cycloheximide [Sigma, C4859] ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µl of secreted medium and 70 µl of LAAO assay solution (0.1 M Tris-HCl pH 8.0, 5 mM L-leucine, 2 mM OPD (o-phenylenediamine dihydrochloride) (Sigma, P9187), 33.3 µg/ml peroxidase (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... along with a photo-initiator 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure2959-410896, Sigma-Aldrich, USA), at a concentration of 0.5 mg/mL followed by exposure to UV-365 for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... cells were grown with light labeled amino acids with both LPS (1 µg/ml) and curcumin (10 µM, Sigma-Aldrich, St. Louis, MO) and heavy labeled amino acids with LPS (1 µg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Microbiology 2020Quote: ... Each strain was grown overnight in GMM (20 mM glucose, 5 mL culture tubes) supplemented with 1x MEM amino acids (AA) (Sigma, Cat. No. M5550). Cells were pelleted ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted to a concentration of 200 μM per amino acid was added to 600 μL 80% HPLC grade methanol (Sigma-Aldrich, 646377-4X4L) and added to cells after draining media and washing with 2-3 mL ice cold saline ...
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bioRxiv - Microbiology 2021Quote: ... SH-SY5Y and Hep G2 cells were maintained in DMEM with 1 % of MEM Non-essential Amino Acid Solution 100x (NEAA) (Sigma Cat. No: M7145), SuBK12-08 and Tb 1 Lu in Eagle’s Minimum Essential Medium (EMEM ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were inoculated into 200 ml MGY liquid media composed of 1.34% yeast nitrogen base without amino acids (Millipore Sigma, St. Louis, MO), 0.4 mg/L biotin ...
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bioRxiv - Pathology 2022Quote: ... 1% penicillin/streptomycin and 1% MEM Non-essential Amino Acid Solution (all three reagents were purchased from Sigma-Aldrich, St. Louis, MO, USA). Cells were cultured at a density lower than 1 × 106/ml to ensure logarithmic growth.
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bioRxiv - Molecular Biology 2022Quote: A 558 bp region of the brm CDS (Flybase: FBpp0075278) encoding amino acids 1321-1506 was cloned into pET28a-6XHis (Sigma, St. Louis, MO). The construct was transformed into BL21-DE3 E ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...