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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for 2 Aminomethyl 1 3 thiazole 4 carboxylic Acid Hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... plates were treated with 3-amino-propyltrimethoxysilane (diluted 1:2 with PBS; Sigma-Aldrich/Merck) for 15min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1% each of phosphatase inhibitor cocktails 2 and 3 (Sigma, Saint Louis, MO, USA). Particulate was removed by centrifugation of lysates at 13,000 rpm for 10 minutes at 4°C and filtration through 0.45 µm syringe filters and lysates were stored at -80°C until preparation for MIB-MS ...
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bioRxiv - Bioengineering 2024Quote: ... spinal cords were subjected to 2-3 incubation periods with DNase (1 mg/mL, Sigma) and the fragments were mechanically digested ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: After 4 hours cells were washed twice with 1 ml Neurobasal medium and changed subsequently to 2 mL of retinoic acid medium made of 1:1 minimal essential medium (MEM) (Sigma-Aldrich) and nutrient mixture F-12 Ham (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... hatching was induced by placing embryos in chilled (4°C) 1 g/L humic acid solution (Sigma-Aldrich, 53680) and incubating them in that solution overnight at room temperature ...
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bioRxiv - Genomics 2024Quote: ... Neurons were observed 4 days after replating aggregates treated with 1 μM retinoic acid (RA, Sigma-Aldrich, reference R2625) for 48 hours ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA with freshly added 1 mM DTT), then concentrated to 500 μl using Amicon Ultra-4 (4 mL, 3 kDa cut-off) (Millipore, Darmstadt, Germany, UFC800324) and loaded onto a Superdex75 10/300 GL size exclusion column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Sigma)10,42 and the metabotropic glutamate receptor antagonist DL-2-Amino-3-phosphonopropionic acid (AP-3) ([1mg/kg] in PBS, stock made in 1M NaOH diluted, pH 7.4; Sigma)10,43 ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were surface sterilized and kept for stratification in dark at 4 °C for 2-3 days on half strength MS-agar (Millipore Sigma) plates ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting EZH2 (shEZH2#3 [TRCN0000040076] and shEZH2#4 [TRCN0000010475]) and FAK (shFAK#2 [TRCN0000121211], shFAK#3 [TRCN0000121318], and shFAK#21 [TRCN0000121321]) were purchased from Sigma-Aldrich. pRK5-TGFβRI-FLAG plasmid (#14833 ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT, 0.5 mg/ml in PBS, Sigma-Aldrich, USA) with 1 μM of menadione (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Immunology 2024Quote: ... a subset of the mice (n = 3-4 per group) received intraperitoneal (i.p.) injections of 2 mg/kg WFA (Millipore-Sigma, #681535), or equivalent volume of the vehicle (VC ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2024Quote: ... 1% Triton X-100] and incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
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bioRxiv - Biochemistry 2020Quote: The pure protein recovered after HPLC purification in 1% acetic acid was concentrated in Amicon Ultra-15 (cut-off 3 kDa) centrifugal filter units (Merck Millipore) to reach the final protein concentration of 1 mM ...
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bioRxiv - Microbiology 2021Quote: ... mobile phase and stained using ethanol: sulfuric acid (70:3 v/v) solution containing 1% (w/v) orcinol monohydrate (Sigma). HPAEC-PAD samples were diluted to 50 μM and resolved on a Dionex PA20 column in a mobile phase of 30 mM NaOH and gradient of 10 mM – 120 mM sodium acetate over 40 min at a flow rate of 0.5 mL min-1 ...
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bioRxiv - Developmental Biology 2024Quote: ... Auxin treatment was performed by transferring worms to bacteria-seeded NGM plates containing 1 mM auxin (indole-3-acetic acid, Sigma). The preparation of auxin stock solution (400 mM in ethanol ...
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bioRxiv - Cell Biology 2024Quote: Worms of the indicated genotype were selected as L4s or young adults (24h post L4 stage) and picked onto NGM plates with and without 1 mM indole-3-acetic acid (auxin, Sigma). Exposure to auxin was carried out for the indicated times ...
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bioRxiv - Microbiology 2023Quote: ... All intracellular metabolite samples were resuspended in 750μL deuterium oxide containing 1mM 3-(Trimethylsilyl)-1-propanesulfonic acid sodium salt (DSS) (178837, Sigma Aldrich) and 50mM NaIO4 (769517 ...
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bioRxiv - Cell Biology 2024Quote: ... After the last EtOH wash the coverslips were incubated with methanol containing 5% acetic acid and 1% (3-Aminopropyl)triethoxysilane (Millipore-Sigma) overnight in a vacuum desiccation chamber in the dark ...
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bioRxiv - Physiology 2022Quote: ... 0.1 M N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) sodium salt (Sigma-Aldrich, H7006), 0.12 M sodium chloride (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... The purified N-glycans were labeled with 2-aminobenzoic acid (2-AA; Sigma-Aldrich, USA) and further purified using a Cyano Base cartridge (Agilent ...
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bioRxiv - Biophysics 2024Quote: ... and buffered with 2 mM N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (TES, Sigma-Aldrich) at pH 7.4 (called Ham’s F12 observation medium).
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bioRxiv - Biochemistry 2022Quote: ... 3-[2-(2-aminoethylamino)-ethylamino] propyl-trimethoxysilane (DETA) (Sigma-Aldrich, Hamburg, Germany) for microtubule immobilization ...
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bioRxiv - Molecular Biology 2024Quote: ... native ChIP was performed using an increasing amount of H3K4me3 antibodies (for the Abcam 0, 2, 4, 6, 10μl, for the Diagenode 0, 4, 8, 12μl and for the Millipore 0, 2, 4, 8, 16μl). The number of gene-specific sequences associated with each quantity of antibody was quantified by real-time PCR targeting the gene encoding the 40S subunit (Forward primer ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated 1-3 overnights at 4°C in the following primary antibodies: goat anti-choline acetyltransferase (1:200; Millipore), rabbit anti-substance P (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Th17 cells were split (usually 1:3) and transduced by adding 1:80-diluted retroviral supernatant and 4 µg/ml polybrene (Sigma) in addition to 10 ng/ml IL-23 and 10 µg/ml anti-IFNγ antibody (all diluted in pre-warmed T cell medium and calculated for culture volume) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μg/mL 4′,6-Diamidin-2-phenylindol (DAPI, Sigma-Aldrich) in 1X PBS was added for 2 min followed by a final washing step ...
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bioRxiv - Biochemistry 2023Quote: ... Irgacure 2959 (2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone) were purchased from Sigma Aldrich for preparation of crosslinked polymer.
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bioRxiv - Microbiology 2020Quote: ... Reduction/alkylation of cysteine residues was performed by addition of Tris(2-carboxyethyl)phosphine hydrochloride and chloroacetamide (Sigma-Aldrich) at final concentrations of 5 mM and 30 mM ...
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bioRxiv - Microbiology 2021Quote: On-bead digestion of the protein on the streptavidin beads was performed as in (Hubner et al., 2015): 2.5 µl of 100 mM Tris(2-carboethyl)phosphine hydrochloride (TCEP, Sigma) was added to a final concentration of 5 mM to the beads and disulfide bonds were reduced for 1 hour while shaking in a thermoshaker at 37°C to prevent settling of the beads ...
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bioRxiv - Genetics 2021Quote: ... The supernatant was transferred to a fresh tube and reduced with 0.5mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma) for 30 minutes at 60°C and alkylated in 3 mM methyl methanethiosulfonate (MMTS ...
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bioRxiv - Cell Biology 2022Quote: ... Disulphide bridges were reduced by the addition of 0.8 μM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma, cat. no. C4706). β-arrestin was labeled by incubation with 0.8 μM Alexa Fluor™ 647 C2 Maleimide (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... samples were mixed with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (Cat. # C4706, Sigma Aldrich) and incubated at 57°C for 45 min ...
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bioRxiv - Biochemistry 2023Quote: ... followed by quenching of 50μL the deuterium exchange reaction mixture in 50μL pre-chilled 50mM sodium acetate quench solution containing 2 M guanidine hydrochloride (Sigma) and 200 mM TCEP ...
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bioRxiv - Cell Biology 2024Quote: ... aSyn N122C variants were stored in phosphate buffer containing 10 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma #646547). Protein concentrations were determined spectrophotometrically by UV absorbance measurements at 280 nm with ε = 5690 M-1cm-1 for wt and all αSyn mutants ...
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bioRxiv - Cell Biology 2024Quote: ... and plated in Induction Medium (Essential 8 Medium,10 μM Chroman1, MedChemExpress, and 2 μg/mL Doxycycline hydrochloride, Sigma) at 400,000 cells per well on double-coated [hESC-qualified Matrigel Matrix and Poly-L-Ornithine (Sigma)] 10 cm tissue-culture treated plates ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was transferred to a fresh tube and reduced with 0.5 mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma) for 30 minutes at 60°C and alkylated in 3 mM methyl methanethiosulfonate (MMTS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The supernatant was transferred to a fresh tube and reduced with 0.5mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma) for 30 min at 60°C and alkylated in 4 mM methyl methanethiosulfonate (MMTS ...
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bioRxiv - Cell Biology 2020Quote: ... or tyramine hydrochloride (Sigma-Aldrich, cat# T2879) was resuspended from powder using HL3.1 solution with 0.35 mM Ca2+ to reach a concentration of 30 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Curare or tubocurarine hydrochloride pentahydrate (Sigma-Aldrich) was dissolved at 50 mM in water ...
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bioRxiv - Neuroscience 2021Quote: ... betaine hydrochloride was purchased from Sigma-Aldrich. HRMS (ESI ...
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bioRxiv - Neuroscience 2021Quote: ... Cytosine β-D-arabinofuranoside hydrochloride (Sigma-aldrich) was added to curb glia proliferation ...
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bioRxiv - Molecular Biology 2021Quote: ... (R)-(-)-phenylephrine hydrochloride (50μM) (cat# P6126, Sigma-Aldrich) or isoproterenol (10μM ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.34 g/L thiamine hydrochloride (Sigma T4625), 0.4% D-glucose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: Histamine hydrochloride (H7250, Sigma, St. Louis, MO), dopamine hydrochloride (H8502 ...
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bioRxiv - Cancer Biology 2020Quote: ... and aminoguanidine hydrochloride were purchased from Sigma and dissolved in water at 10mM,100mM or 1M ...