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Citations for Millipore Sigma :
1351 - 1400 of 7286 citations for Recombinant Salmon il21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 30μL Protein G Agarose beads (Sigma Aldrich) was combined with 3μL of primary antibody and 150 μg of total cell lysate ...
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bioRxiv - Biochemistry 2023Quote: ... SYPRO® Orange Protein Gel Stain (Sigma Aldrich) was used as the reporter dye ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membrane (Millipore, IPVH00010) at 20V ...
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bioRxiv - Immunology 2023Quote: ... Protein G-sepharose beads were purchased from Sigma and molecular mass markers were purchased from Bio-Rad (Hercules ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (Millipore) and immunoblotted with the indicated antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... The protein translation inhibitor cycloheximide (CHX) (Sigma C1988) was used at 20 μg/mL for 6 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... and ubiquitinylated proteins (clone FK2, mouse, Sigma-Aldrich) at 1:200 dilution were added ...
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bioRxiv - Cell Biology 2024Quote: The Protein-G CoIP kit (IP50, Sigma Aldrich) was used for coimmunoprecipitation (CoIP ...
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bioRxiv - Molecular Biology 2024Quote: ... Cas9 protein was purchased from Millipore Sigma (A36498). Transfection of sgRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Magnetic protein A/G beads (Millipore, cat# LSKMAGAG10) were added to the cell extracts and incubated at 4°C for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred onto a PVDF membrane (Millipore), blocked in 5% milk (dry milk diluted in Tris-buffered saline containing 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using Amicon Ultra concentrators (Millipore, molecular weight cut-off 3,000 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were then transferred onto nitrocellulose membranes (Millipore). Non-specific sites were blocked with blocking solution (PBS containing 0.1% Tween20 with 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Protein amount was measured by BCA solution (Sigma). Equal amount of protein samples was separated on SDS-PAGE gel (12% ...
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bioRxiv - Bioengineering 2023Quote: Proteins were reduced (10 mM DTT, EMD Millipore) and alkylated (30 mM iodoacetamide ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and complete EDTA-free protein inhibitors (Sigma-Aldrich). Cell membranes were lysed for 1h at 4°C with gentle rotation on a sample homogenizer ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Genomics 2024Quote: ... The reaction products were analyzed on 8% PAGE-SDS gels with silver staining or Western blotting for PAR using a recombinant anti-poly-ADP-ribose binding reagent (Millipore, Cat# MABE1031; RRID: AB_2665467).
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2021Quote: CDK5 was immunoprecipitated from cells samples at different circadian times (CTs) (circa 200 μg of protein extracts. After immunoprecipitation at 4°C overnight with 2x Protein A agarose (Sigma-Aldrich), samples were diluted in washing buffer and split in two halves ...
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: Protein adsorption to the films was analyzed by incubation in a bovine serum albumin-FITC protein solution (1mg/ml, Sigma Aldrich) overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Microbiology 2021Quote: ... were immunized in a prime-boost-boost regimen with post-fusion hMPV B2 F protein (50 µg protein/mouse) in a water in oil emulsion with Titermax Gold adjuvant (Sigma-Aldrich) via subcutaneous (SC ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Protein content was measured by the BSA (cells) or microBSA (EVs) protein assay according to the manufacturer’s instructions (Sigma Aldrich, USA).
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
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bioRxiv - Systems Biology 2021Quote: ... the gel was scanned for total protein quantification and the proteins were subsequently transferred to an Immobilon-FL PVDF membrane (CAT # IPF00010, EMD Millipore). The membrane was probed with Rabbit anti-RAN (CAT # PA 1-5783 ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: Native PAGE-separated proteins or SDS PAGE-separated proteins were then transferred to PVDF membranes (EMD Millipore, catalog #IPVH00010, lot #R1EB02212) and subjected to Western blotting analysis as described previously (Li et al ...
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bioRxiv - Plant Biology 2023Quote: ... then proteins were extracted by adding 5 volumes of protein extraction buffer containing 1X MS-SAFE protease and phosphatase inhibitor (Sigma MSSAFE) and 1mM PMSF ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: Antigen functional activity was confirmed by assessing ACE2 binding relative to a spike protein positive control (Callaghan Innovation, New Zealand) and ovalbumin negative control protein (Sigma, #A5503). ELISA plates were coated for 1 hour by incubation at ambient temperature with 50 μL antigen (0.072 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Microbiology 2024Quote: ... followed by protein G coupled magnetic beads at room temperature for 10 minutes (PureProteome Protein G Magnetic Bead System, Millipore LSKMAGG). The beads were removed using a magnetic rack ...
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bioRxiv - Microbiology 2024Quote: ... The obtained protein lysates were pre-cleared by centrifugation and incubated with 100 μl (50% slurry) of protein A beads (Millipore Sigma), coated specific antibodies or corresponding species-specific IgG isotype control in 1 ml of 1 × IP buffer ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Prior to labelling the apoplastic fluid was incubated for 30 min with different concentrations of recombinant LpCys1 or chicken egg white cystatin (CEWC, Sigma-Aldrich, St. louis, Mississippi, USA) and Tris-HCl (24 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined using a Bradford protein assay kit (Thermo-Fisher, MA, USA) with bovine serum albumin (Sigma-Aldrich, MO, USA) as the standard ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...