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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Recombinant Mouse CD19 Antigen His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
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bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble fraction was purified by affinity chromatography (either Ni2+ or Strep) (His-Select resin (Sigma-Aldrich); Strep-Tactin®XT 4Flow resin (IBA Lifesciences)) ...
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bioRxiv - Biochemistry 2024Quote: Cleaved MntR was loaded onto a 5 ml column of Ni-NTA His•Bind® Resin (Millipore Sigma) equilibrated with Buffer A and then eluted with 40 mM imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... tissues underwent antigen retrieval by incubating for 15 minutes in hot 10mM sodium citrate buffer pH=6.0 (Sigma cat. no. S-4641). Sections were rinsed in PBS and incubated in 3% H2O2/PBS for 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... sections from the initial portions of sockets were incubated with primary antibody for Proliferating Cell Nuclear Antigen (PCNA) (#MAB424, Merck Millipore, Darmstadt, Germany) diluted to 1:150 in blocking solution and incubated at 4°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval was performed using 1X Sodium Citrate Buffer diluted from a 10X stock (10X Stock: 100mM trisodium citrate (Sigma, Cat#S1804), 0.5% Tween 20 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...
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bioRxiv - Immunology 2020Quote: ... Detection of GFP and Iba1 required high-temperature antigen unmasking in 0.01 M citrate buffer (pH 6.0) (Sigma-Aldrich, San Diego, CA). Sections were treated with biotin and streptavidin blocking solutions (Vector Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... pre-incubation of the neurotransmitter antibodies used in this study with their corresponding antigen (100 μM - 400 μM; GABA (Sigma-Aldrich, A2129), glutamate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... whilst H antigen was detected with 1 μg/ml Ulex Europaeus Agglutinin I (UEA-I) lectin labeled with fluorescein isothiocyanate (Sigma-Aldrich, Germany). Lea and Lex were detected with 0.5 μg/ml of mAbs MEM-158 and 7Le (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The polyreactivity of hybridoma-obtained IgA was tested by adding them to ELISA plates that were coated with common antigens dissolved in PBS before coating ELISA plates: Lipopolysaccharide (Sigma-Aldrich; L4391), double stranded DNA (Sigma-Aldrich ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Microbiology 2023Quote: Proteins in the cell and viral lysates (5 μg of total protein and 10 ng of p24 antigen) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat# IPVH00010). Membranes were blocked with 5% milk in PBS containing 0.1% Tween 20 (0.1% PBST ...
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bioRxiv - Immunology 2023Quote: ... The first kit used was the Milliplex® SARS-CoV-2 antigen panel 1 IgA assay (Millipore Sigma, Cat No. HC19SERA1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in RPMI-1640 complemented with 10% IgG-free Fetal Calf Serum (Sigma-Aldrich, #F1283) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma, SRP0360) were carried out as described previously (Alvarez-Rodrigo et al. ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
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bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Developmental Biology 2019Quote: ... 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml) and 2i (1μM PD325 ...
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bioRxiv - Cell Biology 2019Quote: ... growth media was replaced with media containing 50ng/ml BMP-12 (recombinant human, Sigma Aldrich) and 10ng/ml IGF-1 (recombinant human ...
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bioRxiv - Plant Biology 2019Quote: ... The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore). Protein yield was estimated by measuring absorbance at 595 nm using a spectrophotometer (UV-2600 ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocytes were stimulated with TNF recombinant (10 ng /ml) (Sigma-Aldrich Corporation, Missouri, EUA) or MCM (300 µl ...
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bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma). Fluorescence was measured by flow cytometry 24 h after the addition of RA.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma)) ...
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bioRxiv - Microbiology 2019Quote: Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was purchased from Sigma. The protease inhibitor dec-RVKR-CMK was obtained from Tocris ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Cell Biology 2022Quote: ... subcutaneously with 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) followed by a boost after 4 weeks ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Biochemistry 2020Quote: Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of recombinant protein was induced by 0.2% (w/v) L-Arabinose (Sigma Aldrich). The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.