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1351 - 1400 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019

• Phosphoregulation of the Rad51 auxiliary factor Swi5-Sfr1

  Pengtao Liang, et al., bioRxiv - Biochemistry 2023

  Quote: ... that had been pre-transformed with the pRARE2 plasmid (Novagen). Plasmids used are ...
• Identification of an inhibitory pocket in falcilysin provides a new avenue for malaria drug development

  Grennady Wirjanata, et al., bioRxiv - Microbiology 2023

  Quote: ... Plasmid-containing parasites were selected with 5 µM TMP (Sigma) and 2.5 µg/mL Blasticidin (Sigma) ...
• Simultaneous multi-site editing of individual genomes using retron arrays

  Alejandro González-Delgado, et al., bioRxiv - Bioengineering 2023

  Quote: ... the retron cassette encoded in a pET- 21 (+) plasmid (Novagen) and the CspRecT and mutLE32K in the plasmid pORTMAGE-Ec133 were overexpressed using 1 mM IPTG ...
• CslA and GlxA from Streptomyces lividans form a functional cellulose synthase complex

  Xiaobo Zhong, et al., bioRxiv - Microbiology 2023

  Quote: ... both genes were inserted into the pETDueT-1 plasmid (Novagen) in two steps ...
• Structural and mechanistic insight into ribosomal ITS2 RNA processing by nuclease-kinase machinery

  Jiyun Chen, et al., bioRxiv - Biophysics 2023

  Quote: ... All recombinant plasmids were transformed into E.coli Rosetta (DE3) (Novagen) cells and grown in LB broth at 37°C for 3 h ...
• Conformational states of the microtubule nucleator, the γ-tubulin ring complex

  Brianna Romer, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The plasmid was transformed into Rosetta2 (DE3) pLysS cells (Millipore). Rosetta2 cells were then grown in 4 L of Luria Broth Miller (LB ...
• Crystal structure of a MarR family transcriptional regulator protein

  Arpita Goswami, et al., bioRxiv - Biophysics 2024

  Quote: ... the cloned plasmid was transformed into BL21(DE3) cells (Novagen). Primary culture in LB (Luria Bertani ...
• Horizontally acquired HosA transcription factor bound with 4-hydroxy-benzoic acid exhibits unique tug-of-water dynamics

  Arpita Goswami, Samee Ullah, José A. Brito, bioRxiv - Biochemistry 2024

  Quote: ... the cloned plasmid was transformed into BL21(DE3) cells (Novagen). Primary culture in LB (Luria Bertani ...
• Zika virus remodels and hijacks IGF2BP2 ribonucleoprotein complex to promote viral replication organelle biogenesis

  Clément Mazeaud, et al., bioRxiv - Microbiology 2024

  Quote: ... plasmid was transformed into RosettaTM DE3 competent cells (Sigma Aldrich) by heat shock ...
• Deficiency of Irx5 protects mice from diet-induced obesity and associated metabolic abnormalities

  Joe Eun Son, Kyoung-Han Kim, Chi-chung Hui, bioRxiv - Genetics 2021

  Quote: ... Mouse plasma insulin and leptin levels were determined by using Rat/ Mouse Insulin ELISA Kit (Millipore, EZRMI-13K for insulin and EZML-82K for leptin ...
• Rapid selection of a human monoclonal antibody that potently neutralizes SARS-CoV-2 in two animal models

  Wei Li, et al., bioRxiv - Microbiology 2020

  Quote: ... bound ACE2-mouse Fc was detected by HRP-conjugated anti mouse IgG (Fc specific) (Sigma-Aldrich). Another way to do hACE2 competition ELISA is to incubate the fixed concentration of biotinylated hACE2-Fc with gradient concentrations of antibody ...
• Disruption of the Blood-Brain Barrier in 22q11.2 Deletion Syndrome

  Alexis M. Crockett, et al., bioRxiv - Neuroscience 2019

  Quote: ... Antibodies were diluted in 3% normal donkey serum (monoclonal mouse anti-mouse GFAP 1:2000, Sigma G-A-5 ...
• Platelet Thrombus Formation in eHUS is Prevented by Anti-MBL2

  R. I. Kushak, et al., bioRxiv - Pathology 2019

  Quote: ... rabbit anti-mouse von-Willebrand factor (vWF) and rabbit anti-mouse podocin (Sigma, St. Louis, MO). All secondary IgG class antibodies were from Thermofisher (Waltham ...
• CFAP61 is required for sperm flagellum formation and male fertility in human and mouse

  Siyu Liu, et al., bioRxiv - Cell Biology 2021

  Quote: ... Mouse anti-FLAG M2 (F3165) and mouse anti-Acetylated Tubulin (T6793) were purchased from Sigma-Aldrich. The rabbit antibody specific for the DDDDK-tag (PM020 ...
• Huntington’s disease-specific mis-splicing captured by human-mouse intersect-RNA-seq unveils pathogenic effectors and reduced splicing factors

  Ainara Elorza, et al., bioRxiv - Neuroscience 2020

  Quote: ... mouse anti-RBFOX1 (1:2000 for mouse and 1:1000 for human samples, Merk Millipore, MABE985), mouse anti-RBFOX2 (1:500 ...
• A Back-Door Insights into the modulation of Src kinase activity by the polyamine spermidine

  Sofia Rossini, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and mouse a-mouse IDO1 (clone 8G11, Merk) were conjugated with either PLUS (#DUO92009, Sigma-Aldrich) or MINUS (#DUO92010 ...
• ETV7 limits antiviral gene expression and control of SARS-CoV-2 and influenza viruses

  Heather M. Froggatt, et al., bioRxiv - Microbiology 2020

  Quote: ... and mouse anti-αtubulin (Sigma, T5168). Membranes were washed five times in PBS with 0.1% Tween-20 and then an anti-rabbit-HRP (Thermo ...
• ETV7 limits antiviral gene expression and control of SARS-CoV-2 and influenza viruses

  Heather M. Froggatt, et al., bioRxiv - Microbiology 2020

  Quote: ... and mouse anti-FLAG (Sigma, F3165). Quantitative PCR was performed using SYBR Green (Bio-Rad ...
• The tRNA pseudouridine synthase TruB1 regulates the maturation and function of let-7 miRNA

  Ryota Kurimoto, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and normal mouse IgG (Sigma Aldrich) as an additional negative control ...
• Screening of surface exposed lipoproteins of Leptospira involved in modulation of host innate immune response

  Ajay Kumar, Vivek P. Varma, Syed M. Faisal, bioRxiv - Immunology 2021

  Quote: ... Mouse anti PLG (Sigma SAB1406263-50UG), Plasmin substrate ...
• Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress

  Christopher L. Lord, Susan R. Wente, bioRxiv - Cell Biology 2020

  Quote: ... monoclonal mouse anti-GFP (Millipore Sigma, cat ...
• Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium

  Ozge Z. Aydin, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti alpha tubulin (Sigma, DM1A) at 1:1000 ...
• Coordinated crosstalk between microtubules and actin by a spectraplakin regulates lumen formation and branching

  Delia Ricolo, Sofia J. Araújo, bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-Tau-1 (Sigma Aldrich).
• Intravital imaging of islet Ca²⁺ dynamics reveals enhanced β cell connectivity after bariatric surgery in mice

  Elina Akalestou, et al., bioRxiv - Cell Biology 2020

  Quote: ... and anti-mouse glucagon (Sigma, UK) primary antibody (1:1000) ...
• Fbxo45 binds SPRY motifs in the extracellular domain of N-cadherin and regulates neuron migration during brain development

  Youn Na, et al., bioRxiv - Cell Biology 2020

  Quote: ... and mouse Flag M2 (Sigma-Aldrich). Rabbit Fbxo45 antibody was kindly provided by Kojo S ...
• Puromycin selection of cells with a high expression of the cytochrome P450 CYP3A4 gene activity from a patient with drug-induced liver injury (DILI) and their lifespan prolongation using a combination of CDK4^R24C, cyclin D1 and TERT

  Shoko Miyata, et al., bioRxiv - Cell Biology 2020

  Quote: ... Vinculin (mouse IgG1; SIGMA-ALDRICH; V9264), MCM7 (Mouse MAb IgG1 ...
• PARP12-catalyzed mono-ADP-ribosylation of Golgin-97 controls the transport of E-cadherin

  Giovanna Grimaldi, et al., bioRxiv - Cell Biology 2020

  Quote: ... FLAG-mouse monoclonal antibody (Sigma-Aldrich) was used at 1:5000 for western blotting and at 1:500 for immunofluorescence ...
• Use of dual electron probes reveals role of ferritin in erythropoiesis

  Maria A. Aronova, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse anti-Rab11 (Millipore, Billerica, MA); mouse anti-DDK (4C5 ...
• Loss of the conserved Alveolate kinase MAPK2 decouples Toxoplasma cell growth from the cell cycle

  Xiaoyu Hu, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse m2 anti-FLAG (Sigma #F1804), rabbit anti-Tgβ-tubulin (1:10,000 dilution) ...
• TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALS

  Kathleen M. Cunningham, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-Flag antibody (Sigma #F3165) 1:1000 ...
• Poly(ADP-ribose)-binding and macroH2A mediate recruitment and functions of KDM5A at DNA lesions

  Ramhari Kumbhar, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-Flag (F1804; Sigma-Aldrich), rabbit anti-macroH2A1 (Ab37264 ...
• Fluorescence Correlation and Cross-Correlation Spectroscopy Unveil Cytoplasmic mRNP Composition and Dynamics

  Àngels Mateu-Regué, et al., bioRxiv - Cell Biology 2020

  Quote: ... and FLAG (M2 mouse mAb, Sigma) were incubated overnight at 4ºC ...
• Promyelocytic leukemia nuclear body (PML-NB) -free intranuclear milieu facilitates development of oocytes in mice

  Osamu Udagawa, et al., bioRxiv - Developmental Biology 2021

  Quote: ... anti-mouse PML (#05-718: Millipore), anti-SUMO1 (#4940) ...
• The C-terminus is critical for the degradation of substrates by the Pseudomonas aeruginosa CtpA protease

  Sammi Chung, Andrew J. Darwin, bioRxiv - Microbiology 2020

  Quote: ... or goat anti-mouse IgG (Sigma) horseradish peroxidase conjugates used at the manufacturers recommended dilution ...
• CHEK2 SIGNALING IS THE KEY REGULATOR OF OOCYTE SURVIVAL AFTER CHEMOTHERAPY

  Chihiro Emori, et al., bioRxiv - Developmental Biology 2021

  Quote: ... mouse anti-γH2AX (Millipore, 05-636), rabbit anti-RAD51 (Abcam ...
• Mllt11 regulates the migration and neurite outgrowth of cortical projection neurons during development

  Danielle Stanton-Turcotte, et al., bioRxiv - Developmental Biology 2021

  Quote: ... mouse anti-NeuN (1:200; Millipore), rat anti-Tbr2 (1:200 ...
• The Borrelia burgdorferi adenylyl cyclase, CyaB, is important for virulence factor production and mammalian infection

  Vanessa M. Ante, et al., bioRxiv - Microbiology 2021

  Quote: ... mouse anti-FLAG (1:4000) (Sigma), rabbit anti-P66 (1:5000 ...
• Desmosomes polarize mechanical signaling to govern epidermal tissue form and function

  Joshua A. Broussard, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse anti-vinculin (Sigma-Aldrich, V9131); mouse C4 anti-actin (EMD-Millipore ...
• The E3 ligase TRIM1 ubiquitinates LRRK2 and controls its localization, degradation, and toxicity

  Adrienne E. D. Stormo, et al., bioRxiv - Molecular Biology 2021

  Quote: ... and mouse anti-actin (Sigma, A1978). For western blot ...
• Female meiosis II and pronuclear fusion require Bicaudal-D

  Paula Vazquez-Pianzola, et al., bioRxiv - Developmental Biology 2021

  Quote: ... mouse anti-Flag (1:200, Sigma), mouse anti-V5 (1:200 ...
• Tripartite Inhibition of SRC-WNT-PKC Signalling Consolidates Human Naïve Pluripotency

  Jonathan Bayerl, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-human nuclei (Millipore MAB1281), mouse anti-TUJ1 (Covance MMS 435P) ...
• SIX2 and SIX3 coordinately regulate functional maturity and fate of human pancreatic β cells

  Romina J. Bevacqua, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Mouse anti-Glucagon (1:500, Sigma), Mouse anti-SST (1:500) ...
• SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation

  Jung-Hyun Choi, et al., bioRxiv - Microbiology 2022

  Quote: ... mouse anti-β-actin (Sigma, A5441), rabbit anti-STAT1 (Cell Signaling ...
• A novel fully-spliced accessory gene in equine lentivirus with distinct Rev-responsive element

  Xiangmin Zhang, et al., bioRxiv - Molecular Biology 2022

  Quote: ... mouse anti-FLAG mAb (Sigma, USA), mouse anti-GFP mouse mAb (Affinity Bioscience ...
• A concurrent canonical and modified miRNAome pan-cancer study on TCGA and TARGET cohorts leads to an enhanced resolution in cancer

  Rosario Distefano, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and normal mouse IgG (Millipore Sigma) were employed for immunoprecipitation ...
• A Drosophila Circuit for Habituation Override

  Swati Trisal, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-GFP (Sigma, 1:100), rabbit anti-TH (1:1000 ...
• Antisense oligonucleotides targeting alpha-synuclein reduce pre-formed fibril-induced Lewy pathology and improve some domains of cognitive and motor performance

  Sydney Weber Boutros, Jacob Raber, Vivek K. Unni, bioRxiv - Neuroscience 2020

  Quote: ... GAPDH (mouse, 1:10000, Millipore, # MAB3740), or phospho-histone H2Ax (rabbit ...
• Late adolescence mortality in mice with brain-specific deletion of the volume-regulated anion channel subunit LRRC8A

  Corinne S. Wilson, et al., bioRxiv - Neuroscience 2021

  Quote: ... mouse monoclonal anti-GFAP (Millipore-Sigma, cat ...
• The neural cell adhesion molecule NrCAM regulates development of hypothalamic tanycytes

  Alex Moore, et al., bioRxiv - Neuroscience 2021

  Quote: ... mouse anti-Vimentin (1:200, Sigma); mouse anti-Ki67 (1:500 ...
• Glycation modulates glutamatergic signalling and exacerbates Parkinson’s disease-like phenotypes

  Ana Chegão, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-NeuN mouse (Millipore, 1:400) overnight at 4°C ...