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Citations for Millipore Sigma :
1351 - 1400 of 6718 citations for MXRA8 protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Protein expression was induced in Rosetta2(DE3)pLysS cells (Novagen) at 15°C with 0.1 mM IPTG for 16 hours ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich, UK) for 30 min at 37°C and alkylated in 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (IPVH00010, Immobilon, Millipore) and blocked for 1 h with Tris-buffered saline containing 0.05% Tween 20 and 3% bovine serum albumin ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (IPFL00010, Millipore, USA) and incubated with specific antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins were concentrated using Amicon spin concentrators (Merck Millipore) and subjected to gel filtration (Superdex 75 and 200 16/60 pg ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified from lysate using either nickel (Sigma-Aldrich) or glutathione resin (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... and whole-protein lysate was extracted using Bugbuster reagent (Novagen) and Pierce Universal Nuclease according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using the Crescendo Western Blotting substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... The proteins were then transferred to a PVDF membrane (Millipore) using a semi-dry transfer apparatus at 10V for 30 minutes at room temperature and imaged under the stain-free blot setting on Chemi-Doc to visualize the total transferred proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using the Crescendo Western Blotting substrate (Millipore). The other half of the beads were resuspended in the TRI reagent for subsequent RNA extraction and cDNA synthesis.
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bioRxiv - Neuroscience 2023Quote: ... Total transferred protein was visualized with Ponceau S (Sigma-Aldrich) and incubated overnight in blocking buffer (Intercept Blocking Buffer ...
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bioRxiv - Microbiology 2023Quote: ... proteins were alkylated with 10 mM IAA (Sigma-Aldrich, Germany) in 100 mM TEAB at 25 °C for 20 min in the dark ...
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bioRxiv - Microbiology 2023Quote: ... The resolved proteins were transferred onto a PVDF membrane (Millipore) using a semi-dry blotting system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was expressed E.coli strain Rosetta 2 (DE3) (Novagen). The cells were grown at 37 °C in Luria-Bertani medium (LB ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein G Mag Sepharose beads (Sigma-Aldrich GE28-9513-79) were washed in cell lysis buffer and blocked in 10% BSA (Jackson ImmunoResearch 001-000-162 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein content was previously checked with Ponceau (Sigma-Aldrich, #P7170) and membranes (Merck ...
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bioRxiv - Microbiology 2023Quote: ... followed by transfer of proteins to an Immobilon membrane (Millipore) using CAPS buffer (CAPS 10mM ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were transferred into nitrocellulose membranes (Merck-Millipore, Germany) for 2h at 300 mV using an electrophoretic transfer system (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... followed by protein concentration via Amicon Ultra 10 MWCO (Millipore). EPR Spectroscopy of Spin-Labeled Grh1 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced with 4 mM DTT (Sigma-Aldrich) for 60 min at room temperature and alkylated in the dark using 8 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The eluates were incubated with S-protein agarose beads (Novagen) for 1 h at 4°C and then washed thrice with NETN buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were then transferred onto PVDF membrane (Millipore, IPVH00010) at 400 mAmp for 90 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein complexes were eluted with 3x-FLAG peptide (Sigma-Aldrich) or HA peptide (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were purified from the medium (Ex-Cell405, Sigma-Aldrich) 2 days post-infection by directly flowing over Ni-Penta™ Agarose-Base Resin (Marvelgent Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was induced using 0.5 µg/ml doxycycline (Sigma) for 24 h.
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore) and probed with α-GFP-HRP antibody (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... Proteins were detected by chemiluminescence using HRP Substrate (WBKLS0500, Millipore) in a ChemiDoc Touch imaging system (Bio-Rad) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1000 U/ml ESGRO recombinant mouse LIF protein (Sigma ESG1106), 2 μM PD0325901 (Selleckchem S1036 ...
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bioRxiv - Microbiology 2023Quote: ... Protein was precipitated using ice-cold acetone (Sigma, MS grade), 4 times the volume of the cell lysate and by incubating at -20°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein was visualized using Ponceau S stain (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... Bound proteins were reduced with 5 mM DTT (Sigma-Aldrich) in 50 mM TEAB (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were concentrated using Amicon Ultra Centrifugal Filters (Millipore).
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bioRxiv - Genomics 2023Quote: ... per 20 μl of protein A/G MagnaBeads (Merck Millipore). Beads were then washed once with low salt wash buffer (0.1% SDS ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to a 0.2 μm PVDF membrane (Millipore) and probed with primary antibodies overnight at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... conjugated to protein G agarose beads (Sigma-Aldrich, catalog # IP04). For IP of phosphopeptides containing the (pS/pT)Q and (pS/pT)P phospho-motifs ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 μl of prewashed protein A beads per sample (Sigma; prewashed in lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein solutions were concentrated using Amicon Ultra centrifugal filter (Millipore) for application to FPLC AKTA pure (GE Heathcare) ...
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bioRxiv - Physiology 2024Quote: Protein concentrations were measured using the Bradford reagent (Sigma #B6916). Samples were added at a 1:200 ratio in a 96-well plate and incubated at room temperature for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... then after preclearing with protein A–Sepharose beads (Sigma-Aldrich), HY5 antibody (AS12 1867 ...
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bioRxiv - Neuroscience 2024Quote: ... 2015 using a compartment protein extraction kit (Millipore cat: 2145). Triturated brain tissue was thawed on ice for ∼30 minutes before being homogenized in 2 ml/g of Buffer C (HEPES (pH7.9) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was transferred to an Immobilon PVDF membranes (Millipore Sigma) via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad) ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Cat# IPVH00010) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred to Immobilon-FL PVDF membranes (Merck-Millipore) and washed twice in TBS buffer followed by incubation in Intercept-TBS Blocking Buffer (Li-COR ...
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bioRxiv - Cell Biology 2024Quote: ... Relative protein loading was visualized using Ponceau S Solution (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... protein isolated using RIPA buffer (Pierce) containing protease inhibitors (Sigma) and RNA isolated with TriPure (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was measured using Bradford Reagent (B6916, Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... proteins were transferred to immobilon NC transfer membranes (HATF00010, Millipore) at 400 mA for 2 h in transfer buffer (25 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... was pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were blotted on a PVDF membrane (Merck Millipore, IPVH00010). Membranes were incubated in primary antibodies mouse anti-Vinculin (Sigma ...