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Citations for Millipore Sigma :
1351 - 1400 of 4285 citations for Human MMP24 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... supplemented with 2 % human serum (HS, Sigma-Aldrich) at a concentration of 0.5×106 cells/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... pLKO.1 shp62 (human) was purchased from Sigma. pLKO.1 shp62 (mouse ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Heat-inactivated pooled human sera (Sigma-Aldrich, S1764) and rhesus sera were used as negative controls ...
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bioRxiv - Cell Biology 2023Quote: ... 20ng/mL human fibroblast growth factor (Sigma F5392), B27 (Thermofisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Human skeletal muscle cells (HSMC, Sigma, #150-05F) were maintained in growth medium (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 ng/mL Human Recombinant EGF (Sigma-Aldrich), 100 ng/mL Human Recombinant FGF-10 (Peprotech) ...
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bioRxiv - Microbiology 2024Quote: ... medium supplemented with 4% human AB serum (Sigma), 2 mM L-glutamine (PAA) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μg/ml insulin solution human (Sigma-Aldrich) and 0.5 μg/ml hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml recombinant human EGF (Sigma-Aldrich) and 0.5 μg/ml hydrocortisone ...
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bioRxiv - Biophysics 2024Quote: ... Human Glu-fibrinopeptide (Sigma-Aldrich, St. Louis, MO) served as an internal standard and the peptide signal was recorded throughout all the analysis.
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-human SFTPC (Millipore, ABC99, AR=TE), goat anti-Mrc1/Cd206 (R&D ...
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bioRxiv - Genetics 2024Quote: ... 3% human serum (heat-inactivated from Sigma-Aldrich, St ...
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bioRxiv - Biochemistry 2024Quote: ... and type V (human placenta; Sigma-Aldrich C3657) in aqueous buffer at pH 7.4 (200 mM sodium phosphate ...
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bioRxiv - Microbiology 2024Quote: ... 20% v/v human serum (Sigma, cat# H4522) and penicillin/streptomycin at 37°C in the presence of 5% CO2 for 8 to 10 days ...
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bioRxiv - Immunology 2023Quote: ... and enriched with 8% human serum (Sigma, H4522), 10 mM HEPES (Gibco ...
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bioRxiv - Immunology 2021Quote: ... 0.42ug pVSVg plasmid and 1.3 μg psPAX2 plasmid were together transfected into the HEK923T cells using polyethyleneimine (PEI; Sigma), at a ratio of 1 μg DNA ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated in an endotoxin-free manner (GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit; Sigma-Aldrich) and concentrated using paramagnetic beads (HighPrep PCR Clean Up System ...
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bioRxiv - Cell Biology 2020Quote: ... we used three plasmids: (i) TRC2 pLKO.5-puro empty vector control plasmid (Sigma Aldrich, St. Louis, MO, USA) (ii ...
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bioRxiv - Immunology 2020Quote: ... Plasmids encoding the selected peptides were isolated in 96-well format using the Montage Plasmid MiniprepHTS Kit (MilliPore, LSKP09604) on a MultiscreenHTS Vacuum Manifold (MilliPore ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then transiently transfected with 250ng of empty pEGFPN2 plasmid or pEGFPN2 plasmids coding for MLKL154 or MLKL154 D144K and 250ng of empty pcDNA3.1 plasmid using PEI transfection reagent (Sigma). 24h post-transfection cells were fixed in 4% formaldehyde solution (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid library DNA was purified from twice 5 ml of culture using a GenElute Plasmid Miniprep Kit (Sigma-Aldrich) with elution in twice 20 µl of ddH2O ...
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bioRxiv - Bioengineering 2023Quote: ... Either 15 μg of nanobody Fc plasmid or 1.5 μg of nanobody Fc plasmid and 13.5 μg of ssDNA (Sigma, D7656) were combined with 45 μg PEI (Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... Milliplex Human Angiogenesis / Growth Factor kit Discovery Assay® and the Human Cytokine Array / Chemokine Array (Millipore, St. Charles, MO, USA)] ...
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bioRxiv - Bioengineering 2021Quote: ... Mature human white-like adipocytes were transfected in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522) to mimic the subcutaneous tissue environment ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection for mature human white-like adipocytes was performed in the presence of fresh BM-1 medium supplemented with 1% human serum (Sigma, H4522). Both HeLa cells and mature human white like adipocytes were transfected with LNPs at a final mRNA concentration of 1.25μg/mL ...
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bioRxiv - Pathology 2023Quote: ... adiponectin levels were measured by enzyme-linked immunosorbent assays (Human Chemerin ELISA kit, Biovendor, Brno, Czech Republic; Human Resistin ELISA kit, Millipore Sigma; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit ...
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bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... The human ORF sequence encoding for GSDMD was obtained from the Viral Vector Facility (LUMC) human ORF (cDNA) library from Sigma-Aldrich. The gene ORF was obtained in pDONR223 entry vectors and amplified by PCR with overhang primers to introduce partial sequences of an N-terminal FLAG-tag and C-terminal Myc-tag ...
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bioRxiv - Molecular Biology 2022Quote: ... in a 10-cm dish transfected one day post seeding with packaging plasmids (1 μg VSV.G, 2 μg psPAX2 and 4 μg of transfer plasmid using PEI (Sigma-Aldrich). Virus containing supernatant was collected 72 hours post transfection ...
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: ... The plasmid mixture with the total concentration of 5mg/ml plasmid (equal molar mixture if there are multiple plasmids) containing 1mg/ml Fast green (Sigma) was injected into the embryonic lateral ventricle through a glass pipette ...
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bioRxiv - Bioengineering 2021Quote: ... DNA oligonucleotides and the GenElute HP Plasmid Miniprep and Midiprep kit used for plasmid purifications were acquired from Sigma-Aldrich. Q5 High-Fidelity DNA Polymerase ...
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bioRxiv - Biophysics 2021Quote: ... Cells were harvested by centrifugation at 6000xg for 10min and the pellet was re-suspended in LB/kanamycin for plasmid extraction and purification using a GenElute plasmid miniprep kit (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... we used a dual-luciferase reporting system based on a plasmid containing the sequence for Renilla luciferase and the multiple cloning site (MCS) from the plasmid pMLuc-1 (Novagen) upstream of the firefly luciferase sequence in the plasmid pISO (addgene) ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells that contained pL4440-CP were grown in LB supplemented with carbenicillin (100 μg/mL) followed by plasmid DNA extraction with the High Pure Plasmid Isolation Kit (Sigma). For the in vitro synthesis ...
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bioRxiv - Molecular Biology 2023Quote: ... and was mixed with 1 µg total DNA (100 ng receptor plasmid + 200 ng mGs plasmid + 700 ng salmon sperm DNA (Sigma) solubilized in 100 µL OptiMEM ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Genomics 2024Quote: ... and plasmid DNA was purified from the resulting cell pellet using a GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich #NA0310) following the manufacturer’s recommended protocol with elution in 3 mL H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Plant Biology 2020Quote: ... using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich). Inserts were sequenced (GATC Services ...
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bioRxiv - Biophysics 2021Quote: ... coli expression plasmid vector pET-28b (Novagen, MA) was used to construct all the gp17 WT and mutant clones (37) ...
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bioRxiv - Biochemistry 2019Quote: ... of the plasmid and BacVector3000 baculovirus DNA (Novagen) using the calcium phosphate co-precipitation method ...
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bioRxiv - Microbiology 2019Quote: Expression plasmids were transformed into BL21(DE3) (Novagen) and selected overnight at 37°C on Luria-Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid-pET28a was purchased from Novagen (Germany). Ni-NTA agarose was purchased from G-Biosciences (USA) ...