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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Osteoclast resorbed surface (Oc.S/BS) was measured following TRAP staining with Leukocyte Acid Phosphatase TRAP Kit (Sigma Aldrich, catalog # 387A-1KT). RNA was extracted from isolated whole femur for qPCR for osteoblast (Col1a1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed in 70% ethanol for 1 h and stained for 10 min with an ALP staining solution (BCIP/NBT alkaline phosphatase kit, Sigma-Aldrich), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...
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bioRxiv - Immunology 2019Quote: ... for 6 hours and then exposed to 0.5 × 105 red and 0.5 × 105 blue opsinised (1 hour in 10%v/v human serum) 3 μm diameter latex polystyrene beads (Sigma-Aldrich, Gilligham, UK) such that there were 1 bead per cell ...
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bioRxiv - Neuroscience 2020Quote: ... was used for dual-luciferase assays and candidate compounds were purchased from Sigma (Sulphaquinoxaline ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected and serum insulin levels were determined in triplicate using a rat/mouse insulin Enzyme-Linked Sorbent Assay (ELISA) kit from Millipore (EZRMI-13K) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... Phosphorylated ERK was measured by preparing epithelial cells as described above (Colon epithelial cell isolation) and using a Phospho-Erk1 (pThr202 / pTyr204 + Erk2 (pTyr185/187) and pan-Erk1 / 2 ELISA Kit from Sigma (Cat. RAB0349) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Plasma concentrations of acylated ghrelin and leptin were determined by commercial ELISA kits according to the manufacturers’ instructions (Spi Bio #A05117.96 and Millipore #EZML-82K, respectively).
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bioRxiv - Plant Biology 2019Quote: ... 20 μL/mL Phosphatase Inhibitor Cocktail 2 (Sigma, P5726), 5 mM DTT] was added to the disrupted frozen powders ...
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bioRxiv - Cancer Biology 2021Quote: ... with added protease and phosphatase inhibitor cocktails (Sigma-Aldrich). The protein concentration was determined using a Bradford assay (Boston BioProducts) ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.01% phosphatase-inhibitor cocktail III (all from Sigma). Protein concentrations were measured using a BCA Protein Assay Kit (ThermoFischer Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... with protease and phosphatase inhibitors (Sigma-Aldrich; P8465, P2850). Protein concentration was determined with a BCA protein assay kit ...
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bioRxiv - Developmental Biology 2021Quote: ... in the presence of protease and phosphatase inhibitors (Sigma) and quantified using the Bio-Rad DC protein assay (Bio-Rad) ...
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bioRxiv - Systems Biology 2020Quote: ... Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich; St. Louis, MO), and Protease Inhibitor (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... phosphatase inhibitors (1 mM sodium fluoride [201154; Sigma-Aldrich] ...
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bioRxiv - Immunology 2022Quote: ... and staining substrate (alkaline phosphatase substrate pNPP tablets, Sigma) was added ...
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bioRxiv - Bioengineering 2022Quote: ... and phosphatase inhibitor (1/400 dilution, #P5726, Sigma Aldrich). Lysates were clarified by centrifugation (20,000 × g ...
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bioRxiv - Neuroscience 2021Quote: ... containing fresh protease and phosphatase inhibitor cocktail (EMD Millipore). The lysates were then centrifuged ...
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bioRxiv - Cancer Biology 2019Quote: ... with a mixture of protease and phosphatase inhibitor (Sigma). The total protein concentration of lysate was calculated using BCA protein estimation kit (Pierce) ...
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bioRxiv - Immunology 2019Quote: ... Plates were revealed by phosphatase alkaline substrate (Sigma-Aldrich), and absorbance was measured at 450 nm minus 540 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... alkaline-phosphatase-conjugated anti-FLAG IgG (A9469, Sigma Aldrich) was used at a dilution of 1:5,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with protease and phosphatase inhibitor (Sigma, Cat # P0044,). Extracted cellular proteins from all three time points with appropriate technical and biological replicates were used in an isobaric tag for relative and absolute quantification (iTRAQ ...
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bioRxiv - Immunology 2021Quote: ... supplemented with protease and phosphatase inhibitors (Roche, Sigma-Aldrich). Lysates were mixed 1:1 with 2x reducing sample buffer (320 mM Trizma base (pH adjusted to 6.8) ...
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bioRxiv - Immunology 2021Quote: ... pH 8.0) with phosphatase and protease inhibitors (Sigma-Aldrich) for 5 mins at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Calf intestinal alkaline phosphatase (CIAP) was purchased from Sigma. Clostridium perfringens (C ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% of phosphatase inhibitors cocktails I & III (Sigma) was added ...
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bioRxiv - Biochemistry 2021Quote: ... and protease and phosphatase inhibitors (equivalent to Sigma-Aldrich plant protease inhibitor cocktail and phosphatase inhibitor cocktails #2 and #3 ...
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bioRxiv - Biochemistry 2020Quote: ... Alkaline phosphatase conjugated anti-Rabbit IgG (1:5000) (Sigma) was used as a secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5) containing phosphatase and protease inhibitors (Sigma-Aldrich). Samples were then homogenized via ultrasonication ...
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bioRxiv - Cancer Biology 2021Quote: ... and phosphatase inhibitors (1:500; Sigma, P5726 and P0044). Western Blots were performed following standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... and Phosphatase Inhibitor cocktail (P0044, Millipore Sigma, Burlington, MA,). Equal amounts (30 μg ...
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bioRxiv - Cell Biology 2021Quote: ... and phosphatase inhibitors (Sigma-Aldrich, cat. no. PHOSS-RO), and incubated on ice for 45 minutes with brief vortexing every 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and rabbit anti-bovine alkaline phosphatase conjugated secondary (Sigma). Mouse antibody isotypes were determined using the mouse monoclonal antibody isotyping kit (Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... solution containing 1x PhosSTOP™ phosphatase inhibitor (Sigma Aldrich) / cOmplete™ protease inhibitor (including EDTA ...
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bioRxiv - Cancer Biology 2021Quote: ... Phosphatase Inhibitor Cocktail 2 solution (1/100, SIGMA-Aldrich) and one tablet of cOmplete EDTA-Free protease inhibitor cocktail (SIGMA-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 1% protease and 1% phosphatase inhibitor cocktails (Sigma). Protein samples were quantified using the Bradford assay reagent (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... and phosphatase inhibitor cocktails I and II (Sigma-Aldrich). Cells in the lysis buffer were mechanically ruptured by vigorous pipetting and passing through a small gauge (29G ...
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bioRxiv - Cell Biology 2021Quote: ... and optionally with Phosphatase Inhibitor Cocktail 1 (Sigma, P2850). Lysates were prepared with SDS sample buffer (Novex ...
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bioRxiv - Cell Biology 2020Quote: ... 2% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich) and 500 U/ml universal nuclease ...
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bioRxiv - Molecular Biology 2022Quote: ... and PhosSTOP™ Phosphatase Inhibitor tablets (Roche; Sigma-Aldrich). The lysed supernatant was obtained by centrifugation at 4°C for 10 min at 13,000 rpm and the total protein content was quantified by a Micro BCA Protein assay kit (Thermo Scientific) ...
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bioRxiv - Genetics 2021Quote: ... with added MS-SAFE Protease and Phosphatase Inhibitor (Sigma). Following low speed centrifugation to pellet cell debris (1,200g ...
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bioRxiv - Genetics 2021Quote: ... with added MS-SAFE Protease and Phosphatase Inhibitor (Sigma) for 30 minutes on ice with regular vortexing ...
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bioRxiv - Cell Biology 2020Quote: ... plus protease/phosphatase inhibitors (#P8340/#P0044, Sigma-Aldrich, USA). Whole-muscle and heart extracts were obtained by homogenization at 4°C in RIPA 1x lysis buffer plus protease/phosphatase inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... with freshly added 1x phosphatase inhibitor cocktail (Sigma-Aldrich). Lysates were clarified by centrifugation at 16,600 g at 4°C for 20 min and supernatants were quantified by BCA assay.
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bioRxiv - Neuroscience 2022Quote: ... phosphatase inhibitor cocktail 2 (1:100 dilution, P5726: Sigma), and phosphatase inhibitor cocktail 3 (1:100 dilution ...
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bioRxiv - Genetics 2022Quote: ... 200 ul alkaline phosphatase blue microwell substrate (Sigma, AB0100) was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with anti-protease and anti-phosphatase mixture (Millipore) at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... 1% sodium deoxycholate with additional phosphatase inhibitors (Sigma, P0044). Detergent soluble lysates were sonicated and centrifuged to remove cell debris ...
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bioRxiv - Developmental Biology 2019Quote: ... Detection was via alkaline phosphatase-conjugated anti-digoxygenin (Sigma), followed by NBT/BCIP color reaction (Boehringer Mannheim ...