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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Wnt display was confirmed by mouse Monoclonal ANTI-FLAG M2-FITC antibody (FLAG-FITC, Sigma F4049), showing comparable display between the full-length and NC-linker truncated versions.
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bioRxiv - Plant Biology 2019Quote: ... proteins in fusion with 2xStrep-FLAG were purified with Anti-FLAG M2 Magnetic Beads (Sigma Co.). Purification was carried out according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-FLAG M2 affinity gel (A2220) and 3 x FLAG peptide (F3290) were from Sigma-Aldrich, protein A or G beads were from Invitrogen (10004D) ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitation against flag was performed using EZview™ Red ANTI-FLAG® M2 Affinity Gel (Sigma) overnight at 4°C followed by washing in IP buffer ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged Srb10/ Cdk8 was recovered by immunoprecipitation with anti-FLAG-conjugated M2 agarose beads (Sigma). Samples were washed two times in KLB followed by two times in kinase assay buffer (KAB ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of FLAG/HA-AGO2 was performed with Anti-FLAG M2 Magnetic Beads (Sigma Cat # M8823). Beads were washed twice with TBS buffer (50 mM Tris HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-Tel1 immunoprecipitation was performed with 2µl of anti-FLAG antibody (M2 monoclonal, Sigma, Ref F1804) and incubation at 4°C for 2 hours ...
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bioRxiv - Biophysics 2019Quote: ... followed by pelleting ribosomes through a sucrose cushion and Western blotting with an anti-FLAG antibody (Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody (SIGMA)) and RPS19 antibody (Bethyl Laboratories ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... An anti-FLAG IP to recover labelled EFR3B was performed using 500 μg of sample and 20 μL of anti-FLAG bead slurry (EZview Red ANTI-FLAG M2 Affinity gel from Millipore Sigma). Samples were left to rotate for 2 h at 4 °C in a cold room ...
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bioRxiv - Plant Biology 2019Quote: ... the filtered inflorescence sap was directly used as reducing agent to synthesize AgNPs using AgNO3 (SIGMA) as precursor ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag-tagged SecPH proteins in the cell extracts were captured with an anti-Flag M2 affinity gel (ANTI-FLAG M2 Affinity Gel, Sigma-Aldrich, A2220) by incubation on a rotating wheel at 4°C for 3 h ...
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bioRxiv - Bioengineering 2022Quote: ... we used 4% agarose micro-beads bound to the Anti-FLAG M2 monoclonal antibody (Anti-FLAG® M2 Magnetic Beads from Sigma, M8823). Beads size ranges between 20-75 μm ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... were incubated for 2h at 4°C with 15 µL anti-FLAG conjugated Sepharose beads (ANTI-FLAG® M2 affinity gel, Sigma, A2220) in 500 µL NENT-100 (20 mM Tris HCl [pH 7.4] ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Immunology 2019Quote: ... Anti-FLAG (M2) and anti-Myc agarose beads were purchased from Sigma and Thermo Fisher (Waltham ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-pERK1/2 (T185/Y187) and mouse anti-Flag M2 (Sigma); and mouse anti-H3 and rabbit anti-H3K4 trimethyl (Active Motif).
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bioRxiv - Cell Biology 2019Quote: Anti-FLAG (3165) and anti-Myc (4439) antibodies were purchased from Sigma. Anti-HA antibody was purchased from Cell Signalling (C29F4) ...
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bioRxiv - Genetics 2019Quote: ... These membranes were probed with anti-Flag (Sigma, anti-mouse 1:1000), anti-Pan-Ras (Millipore ...
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bioRxiv - Physiology 2021Quote: ... Either anti-SWELL1 (1:400) or anti-Flag (1:1500, Sigma #F3165) antibody were added to the cells and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were clarified and Flag-tagged protein were precipitated using anti-Flag M2 magnetic beads (Sigma Aldrich). After several washes in IP buffer ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged RtcB proteins were affinity-purified from the lysates with anti-Flag M2 affinity-gel (Sigma) followed by extensive washes ...
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bioRxiv - Cell Biology 2021Quote: Mrc1-FLAG in immunoprecipitation and WCE samples was detected by rabbit anti-FLAG (1:3000, F7425, Sigma) followed by horseradish peroxidase (HRP)- conjugated swine anti-rabbit (1:5000 ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were detected with horseradish peroxidase (HRP)-conjugated anti-FLAG antibody (Sigma Aldrich, A8592-1MG). All western blots were developed using the ECL Plus Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged Arc was immunopurified using 50 µL of anti-FLAG M2 Magnetic Beads (Sigma-Aldrich, M8823) while endogenous TNIK was pulled-down with 10 µL of anti-TNIK antibody (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... FLAG®-tagged MprF was detected both by a mouse anti-FLAG® primary antibody (Sigma-Aldrich) and goat antimouse secondary antibody (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: Flag-tagged proteins were captured from 1 mg total cell lysate using anti-Flag affinity matrix (Sigma) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged zebrafish Grk1a or Grk1b was isolated using anti-FLAG M2 Affinity Gel (#A2220) from Millipore-Sigma as described previously (15 ...
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bioRxiv - Microbiology 2021Quote: ... Fusion FLAG-ATG5 and ATG7 proteins were captured using Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich) according to the manufacturer's instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of Flag-tagged proteins were carried out with the anti-FLAG M2 beads (Cat. A2220, Sigma) in RIPA buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma, #SAB4301135). Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... Flag-tagged proteins were detected using the anti-Flag HRP-coupled antibody (mouse monoclonal, A8592; Sigma-Aldrich) diluted 1:5000 (v/v) ...
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bioRxiv - Biochemistry 2020Quote: ... Phage with uncleaved FLAG-tag were removed by immunodepletion using M2 anti-FLAG monoclonal antibody (Sigma-Aldrich) coupled to epoxy-activated M-450 magnetic beads (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti- FLAG M2 magnetic beads were incubated with 100 µg/ml 3x FLAG peptide (Sigma, #F4799- 25MG) in FIB buffer + 10% glycerol for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... CTRL vector or TFEB-FLAG lysates were incubated with M2 anti-FLAG agarose beads (A22220, Sigma Aldrich) for 3 min at 4°C on wheel ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble protein fraction was incubated with Lysis buffe-equilibrated FLAG beads (Anti-FLAG M2 Agarose, Sigma) for 3 h at 4°C on a rotating wheel ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble protein fraction was incubated with Buffer C-equilibrated FLAG beads (Anti-FLAG M2 Agarose, Sigma) for 3 h at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged proteins were incubated with 30 μl pre-washed anti-FLAG M2 affinity gel (Sigma, #A2220) for 3 h at 4 °C and washed four times with IP wash buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged recombinant substrates were purified by immunoaffinity chromatography using the anti-FLAG M2-agarose beads (Sigma), according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-Tel1 and Hsp104 were detected with an anti-FLAG mouse antibody (M2 monoclonal, Sigma, 1/3000) or anti-Hsp104 rabbit antibody (polyclonal ...
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bioRxiv - Microbiology 2020Quote: ... Anti-FLAG antibodies (1:5000; Sigma-Aldrich, Shanghai, China) were then added to the PBSTM ...
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bioRxiv - Cell Biology 2019Quote: ... then 250 ul anti-FLAG M2 affinity resin (Sigma) was added for 2 hr at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-Flag (Sigma, clone M2 F3165, 1: 500). Bands were visualized by horseradish-peroxidase-conjugated sheep anti-mouse IgG antibodies (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-FLAG (Sigma-Aldrich, F3165; WB 1:2000), mouse anti-Hec1 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Flag-M2 (F3165, Sigma-Aldrich, St. Louis, MO) or IgG control ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal antibody anti-FLAG M2 (from Sigma-Aldrich). In these experiments ...
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bioRxiv - Cell Biology 2020Quote: ... and mouse monoclonal anti-flag (1:500; Sigma, F1804), and secondary antibodies included Alexa594-conjugated goat anti-rabbit (1:2000 ...