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Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for 2 Ethyl 3 ethyl d5 pyrazine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The remaining RBC pellet was suspended in 2 mL of incomplete media and then gently layered on to a 3 mL aliquot of percoll (Sigma Aldrich) diluted with 1X RPMI (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2024Quote: ... 1% Triton X-100] and incubated for 2-3 hours at 4°C with either 5 μl of anti-FLAG-M2 affinity resin (Sigma-Aldrich), Streptactin sepharose (IBA Lifesciences) ...
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bioRxiv - Microbiology 2024Quote: ... Larvae of indeterminate sex were infected by injection of 10nL into the caudal vein at 3 days post fertilization (dpf) using a capillary needle containing bacteria diluted in PBS + 2% phenol red (Sigma #P3532), as previously described [104] ...
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bioRxiv - Microbiology 2024Quote: ... Measurement of hexosamines (amino sugars) from the deacetylated samples was done by treatment with 3-methyl-2-benzothiazolone hydrazine hydrochloride (MBTH, Sigma-Aldrich) under mildly acidic conditions ...
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bioRxiv - Immunology 2024Quote: ... a subset of the mice (n = 3-4 per group) received intraperitoneal (i.p.) injections of 2 mg/kg WFA (Millipore-Sigma, #681535), or equivalent volume of the vehicle (VC ...
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bioRxiv - Synthetic Biology 2024Quote: ... All the reagents were prepared in enough amounts for all the experiments for the 2 systems of colicin M and E1 except for the 3-PGA (Sigma Aldrich) was used for colicin M while creatine phosphate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with TBS-T (TBS + 0.05% Tween20) (3 times x 2 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Plain media was added overnight and on day 3 cells were passaged into selection media containing 2 µg/uL puromycin (Sigma P8833) and 150 µg/mL hygromycin B (Thermo 10687010 ...
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bioRxiv - Cancer Biology 2024Quote: ... livers were washed 3 times with PBS and incubated 1 hour at room temperature with blocking solution (PBS containing 2 % BSA, Sigma Aldrich) followed by incubation at 4 °C overnight with primary antibody staining (blocking solution containing 1:100 primary antibody) ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids grown in collagen for 3/6 days underwent collagen digestion by incubation in DMEM without serum supplemented with 2 mg/ml collagenase (Sigma, C2139) for 45 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Osteogenic differentiation was performed in osteogenic medium for 2 weeks (medium was changed every 3 days) and quantified by alizarin red S staining (Sigma, A5533). The osteogenic differentiation medium contained DMEM supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... After 72 hrs we performed 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays using the Roche MTT protocol (Sigma Aldrich Cat#11465007001). After 24 hours light absorption at 570 nm was measured using the MTT plate reader (Wallac EnVision 1.12) ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Cancer Biology 2020Quote: ... The antibody reaction was visualized with 3-3’ diaminobenzidine (Sigma, D8001) followed by counterstaining with hematoxylin ...
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bioRxiv - Neuroscience 2022Quote: ... prior to being rinsed and reacted using 3-3’diaminobenzidine (Sigma) as chromagen ...
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bioRxiv - Biochemistry 2021Quote: ... and 3’,3’c-di-AMP were purchased from Sigma-Aldrich, 2’,3’-cGAMP ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimidehydrochloride (EDC) were procured from Sigma Aldrich, USA.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT, Sigma). Selection plates were left for 8 days at 30°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate hydrate (CHAPS) (C3023; Sigma Aldrich), and sodium dodecyl sulfate (SDS ...
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bioRxiv - Systems Biology 2024Quote: ... 5µM 6-bromoindirubicin-3-oxime / GSK-3 inhibitor (Sigma, 361550-1MG) and 100 µg/ml Primocin (InVivogen ...
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bioRxiv - Systems Biology 2024Quote: ... or 3-bromopyruvate (3-BP; Sigma-Aldrich, St. Louis, MO; 16490) were added to their respective wells ...
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bioRxiv - Biophysics 2024Quote: ... 15 mM 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide (Millipore Sigma E6383), 5 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Cell Biology 2020Quote: Cultured CT26 cells were collected and their viability was assayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, St. Louis, MO, USA) colorimetric method ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed with PBST 2-3 times and bands were visualized upon addition of SigmaFast BCIP®/NBT tablets (Sigma B5655) (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of freshly made 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 1 mg/mL; Sigma-Aldrich, St. Louis, MO) in serum-free ...
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bioRxiv - Biophysics 2020Quote: ... oocytes were incubated twice (1st 45 min, 2nd variable) in OR-2 containing 3 mg/ml collagenase type IV (Sigma, C-9891). Defolliculated oocytes were stored in ND96 solution containing (in mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Cancer Biology 2021Quote: ... Viable cells were determined post treatment using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, USA; Cat# M2128) assay by measurement of absorbance at 570 nm.17 The relative rate of cell growth for each cell line was factored into the analysis by subtracting the absorbance at time 0 from both the control and treatment groups ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Plant Biology 2022Quote: Kinase activity assay was carried out by mixing 2 μg of CORK1KD or GST-CORK1KD-G748E with 3 μg of myelin basic protein (MBP; Sigma-Aldrich, Germany) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
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bioRxiv - Cell Biology 2024Quote: ... logarithmically growing vps4Δ CDC48 or cdc48-3 cells were shifted to 37 °C for 2 h and simultaneously treated with 50 µM MG-132 (Sigma, Cat. # 474787) or the respective vehicle ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips was functionalized by amine using the silanization solution of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Sigma-Aldrich, 8191720100), acetic acid ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cellular dehydrogenase activity was analyzed by the conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, St. Louis, MO) as described.[22e ...
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bioRxiv - Microbiology 2024Quote: ... Cells were blocked for 2 hr with 3% BSA and incubated overnight with 1:500 dilution of rabbit anti-His antibody (Sigma-Aldrich SAB5600227). Cells were washed again and incubated for 1 hr with a 1:1000 dilution of either anti-rabbit FITC (Abcam 6717 ...
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bioRxiv - Cancer Biology 2024Quote: ... Target site on CCNB1 was amplified by a 2-step PCR using KOD XTREME HOT START DNA POLYMERASE (Sigma Aldrich #71975-3) using 25% of the total cDNA reaction above ...
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bioRxiv - Immunology 2024Quote: ... paraffin-embedded (FFPE) and blocks cut at 5µm (2-3 cuts per slide) and stained with Masson’s Trichrome stain (Sigma; Cat. No. HT15-1KT) following manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Samples were stained with 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542) for 5-10 minutes at room temperature manually and rinsed three times with 1xTBS with 0.1% (v/v ...
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bioRxiv - Genomics 2024Quote: ... formamide in 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542). Readout probes ...
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bioRxiv - Plant Biology 2024Quote: ... To the finely ground plant material 300 µL urea/DTT extraction buffer (8M urea/5mM DTT in 100mM Tris, pH 8.5/ protease inhibitor cocktail 2 and 3, Sigma, 20 µL/mL) was added ...