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Citations for Millipore Sigma :
1301 - 1350 of 7667 citations for Recombinant Ovine IL4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were extracted using RIPA buffer (EMD Millipore) supplemented with protease and phosphatase inhibitors as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF membranes (MERCK-Millipore) using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Proteins were reduced with 10 mM dithiothreitol (Sigma) for 1 h at 56 °C and then alkylated with 20 mM iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to Immobilon-P membranes (Millipore) using a semi-dry transfer system from Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... The solubilized protein was isolated using FLAG (Sigma) affinity gel ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was transferred to a PVDF membrane (Millipore), blocked with 5% BSA in TBS with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were detected using Luminata Forte (Millipore, WBLUR0500) and quantification was performed by densitometry using QuantityOne software (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Single-strand binding protein from Sigma-Aldrich (S3917) was added at the final concentration (in Mixture A+B reaction ...
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bioRxiv - Genetics 2023Quote: ... Protein concentration was measured using Bradford reagent (Sigma) and the entire L1 lysates were added to 25 ul magnetic Protein-G beads (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein concentration was measured using BSA (Sigma-Aldrich) serial dilutions and the “DCTM Protein Assay” kit (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted onto polyvinylidene difluoride membranes (Millipore). The membranes were probed with the indicated antibodies (all from Cell Signaling) ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein was transferred to Immobilon-FL membrane (Millipore). To detect tubulin ...
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bioRxiv - Microbiology 2023Quote: ... 30 µl of agarose protein A beads (Millipore) washed with RNA extraction buffer were added to 1 mg of protein lysate for a 1.5 h incubation at 4 ℃ on a rocking platform ...
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinyldienefluoride membranes (Millipore #IPFL00010) and subjected to western blotting as described previously5.
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bioRxiv - Biochemistry 2023Quote: Active SLO protein was obtained commercially (Sigma Aldrich) or expressed and purified recombinantly in-house ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were overexpressed in E.coli Rosetta (DE3) (Novagen) cells and cultured in LB broth to an OD600 of 0.6-0.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were reduced using 1 mM DTT (Sigma) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the Bradford (Sigma) method using 2 mg/mL BSA as a standard (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... anti-Protein A antibodies (Sigma, P3775, 1:5000). IRDye secondary antibodies (800CW and 680RD ...
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bioRxiv - Immunology 2023Quote: ... Protein G-sepharose beads were purchased from Sigma and molecular mass markers were purchased from Bio-Rad (Hercules ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membrane (Millipore, IPVH00010) at 20V ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were transferred onto Immobilon-P membranes (Millipore) and probed with anti-rabbit-peroxidase (Jacksons) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to a PVDF membrane (Millipore), blocked by blocking buffer (Rockland ...
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bioRxiv - Cell Biology 2023Quote: ... and then with Protein A Magnetic Beads (Sigma) for one hour.
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bioRxiv - Biochemistry 2023Quote: ... SYPRO® Orange Protein Gel Stain (Sigma Aldrich) was used as the reporter dye ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to a PVDF membrane (Millipore) and immunoblotted with the indicated antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... and biotinylated-Hyaluronan Binding Protein (Millipore, 1:50) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred to nitrocellulose (Sigma #10600001) using a wet transfer apparatus (Bio-rad #1704070) ...
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bioRxiv - Cancer Biology 2024Quote: ... Parallel protein A/G beads (EMD Millipore, #IP0515ML), H3K4Me (Cell Signalling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were wet transferred onto PVDF membrane (Millipore) for 1 h at 100 V ...
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bioRxiv - Neuroscience 2024Quote: Proteins were reduced (10 mM DTT, EMD Millipore) and alkylated (30 mM iodoacetamide ...
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bioRxiv - Cancer Biology 2024Quote: ... 50μg protein emulsified with complete Freund’s adjuvant (Sigma) was subcutaneously (s.c. ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% protein inhibitor cocktail (Sigma- Aldrich: P8849)] ...
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bioRxiv - Cell Biology 2024Quote: ... and proteins alkylated with 55 mM iodoacetamide (Sigma) in 100 mM NH4HCO3 for 20 mins at RT (protected from light) ...
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bioRxiv - Neuroscience 2024Quote: ... using BCA Protein Assay (Sigma-Aldrich, CA, USA). For all calculations ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a PVDF membrane (Millipore). Membranes were blocked with 5% (wt/vol ...
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bioRxiv - Neuroscience 2024Quote: Protein was extracted in RIPA buffer (R0278, Sigma) supplemented with protease inhibitor cocktail (P8340 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto polyvinylidene fluoride membrane (Millipore). Membranes were blocked with 10% non-fat milk in tris-buffered saline containing 0.3% Tween 20 (TBST ...
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bioRxiv - Systems Biology 2024Quote: ... The proteins were reduced with TCEP (Sigma-Aldrich), final concentration 5 mM for 30 min at 37 °C and alkylated with 10 mM IAA (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1% protein inhibitor cocktail (100×) (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was quantified using BCA1 from Sigma. Thirty micrograms of protein lysate were separated by 6% Bis-Tris Gel ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... The reaction products were analyzed on 8% PAGE-SDS gels with silver staining or Western blotting for PAR using a recombinant anti-poly-ADP-ribose binding reagent (Millipore, Cat# MABE1031; RRID: AB_2665467).
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).