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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for Recombinant Mouse CES2E Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Plant Biology 2021Quote: ... Substrate specificity of recombinant AtPME2 activity was determined at pH 7.5 and 28°C using commercial citrus pectin (Sigma-Aldrich, DM >85% ...
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bioRxiv - Bioengineering 2021Quote: ... and 1,500 mg/L sodium bicarbonate (ATCC) supplemented with 2 ng/ml recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (Sigma-Aldrich) and 10 % fetal bovine serum ...
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bioRxiv - Plant Biology 2021Quote: ... substrate specificity assays for recombinant PcIBMS1 and PcIPMS1 were determined with a spectrophotometric end-point assay with DTNB (Sigma-Aldrich). An aliquot of 1-5 μL of the protein preparation was added to an enzyme assay mixture (100 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... pFPV-TurboFP650 recombinant plasmids were selected on Trypticase Soya Agar (TSA - BioMérieux) containing 100 µg/mL of carbenicillin (Sigma-Aldrich) and clones which showed a purple color were selected for restriction analysis ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Biochemistry 2019Quote: ... STAT1 (0.3 µM) (Biosource, PHF0011) was phosphorylated in vitro by incubation with recombinant human cdk1/cyclinB (0.2 µM) (Millipore, 14-450) at 30 °C for 90 min in the presence of 1 mM ATP ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were digested for at least 14 h (overnight) with an animal-free recombinant bovine trypsin expressed in corn (Trypzean T3568 from Sigma) at 1/50 w/w and RT ...
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bioRxiv - Bioengineering 2020Quote: ... incubated 30 seconds in plasma cleaner then coated with cell-specific adhesive surface: for at least 1 hour with 10 μg/ml recombinant human fibronectin (Sigma) for Hela and SHSy-5Y cells ...
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bioRxiv - Microbiology 2020Quote: ... chondrophila were first evidenced by incubating the purified recombinant enzymes overnight at 30°C with 10 mg.mL-1 glycogen from rabbit liver (Sigma-Aldrich) and 20 mM inorganic phosphate in 20 mM Tris-HCl buffer (pH 6.8) ...
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bioRxiv - Microbiology 2021Quote: ... kinetics was recorded as the HA loaded sensors were dipped into 1.5-fold serially diluted concentrations of recombinant GM2 (Sigma Aldrich). The reaction was carried out in a 1x kinetics buffer comprising of 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Purification of the recombinant mucoricin was performed by Ni-NTA matrix affinity purification according to the manufacturers’ instructions (Sigma-Aldrich). The purity of the protein was confirmed by SDS-PAGE and quantified by a modified Lowry protein assay (Pierce).
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... PDAC cells were treated with human recombinant FasL (MiaPaCa2: 50 ng/mL; TKCC10: 100 ng/mL) (Sigma-Aldrich, cat. SRP3036), 48 hours post transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: BMDM were polarized or not with recombinant IL-4 (10 ng mL−1, peprotech) for 24 hours and with LPS (10 ng/ml, Sigma) for 8 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Cell Biology 2019Quote: ... or western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-myc from mouse (9E10 ...
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bioRxiv - Cancer Biology 2021Quote: ... antibody overnight and then with equilibrated protein G beads (Millipore Sigma, Protein G Agarose, Fast Flow) for 2h ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein-protein interactions were assessed using the PLA Duolink in situ starter kit (Sigma Aldrich, DUO92101) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich Cat# BCA1) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296) at 4°C for 1 hr for preclearing ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µg of Rab protein were mixed with 5x SYPRO® orange protein gel stain (Sigma) in a volume of 20 µl ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of the collagen was verified by both BCA Protein assay (Sigma-Aldrich, cat: BCA1) and drying via vacuum centrifugation.
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bioRxiv - Cell Biology 2022Quote: ... and the protein concentration of the supernatant assessed by Pierce 660nm protein assay reagent (Sigma, 22660). All samples were diluted to a final 1X Laemmli sample buffer (40% glycerol ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined in the supernatants using Bradford protein assay method (Bradford reagent, Sigma–Aldrich), a standard curve was performed using BSA (bovin serum albumine ...
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bioRxiv - Physiology 2023Quote: ... The total protein content in samples was obtained using a BCA protein assay kit (Sigma-Aldrich) with the SpectraMax Paradigm microplate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2022Quote: ... AviTagged trimeric S and RBD proteins were biotinylated using the Enzymatic Protein Biotinylation Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... 9 mg of protein were mixed with 20 µL of S-protein Agarose beads (#69704, Novagen), that had been previously washed in extraction buffer with 1% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... The protein pellet was washed in protein wash buffer (0.3 M guanidine hydrochloride (Sigma Aldrich, USA) in 95% ethanol ...
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bioRxiv - Biophysics 2023Quote: ... The fusion protein was treated with SUMO protease (Sigma-Aldrich SAE0067, 5 U/ mg target protein) to liberate 3CLpro with authentic N- and C-termini (Ser1 and Gln306 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (DM1A, dilution 1:5000) and mouse anti-FLAG M2 antibody (Sigma); Goat Anti-Rabbit IgG(H+L ...
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bioRxiv - Cell Biology 2020Quote: ... 1:1000 anti-HA Mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma Aldrich) or 1:1000 anti-ACP antibodies (a kind gift from Prof ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 (mouse mAb clone E-7, Santa Cruz sc-13162; mouse mAB, clone AR12, Millipore UK Ltd ...
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bioRxiv - Cell Biology 2020Quote: Mouse tracheal epithelial cells (mouse AECs) were isolated after pronase (Sigma-Aldrich, 9036-06-0) digestion of WT and Atg16L1HM mouse tracheas and grown on supported membrane inserts (Corning #3460 and #3470 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Anti-mouse BKα mouse monoclonal antibody (L6/60, Millipore; 1 in 1000 Wetsern blot (WB), 1 in 750 in Immunofluorecence (IF)) ...
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bioRxiv - Cancer Biology 2021Quote: ... and mouse anti-mouse α–SMA (1:400; clone 1A4; Sigma-Aldrich Chemie Taufkirchen, Germany) was conducted 45 min at RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mouse anti-Flag M2 and mouse anti-β-actin (AC-15) were from Sigma Aldrich.
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin and mouse anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA); mouse anti-His (CoWin Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Either mouse M2 Flag or mouse HA-7 primary antibodies (Sigma-Aldrich, St. Louis, MO) were used at a 1:1000 dilution in 2.5% BSA in TBST for 1 hour at room temperature ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... mouse monoclonal anti-β actin and mouse monoclonal anti-FLAG (clone M2) were from Sigma; rat monoclonal anti-CD40 (3/23) ...
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bioRxiv - Neuroscience 2023Quote: ... pre-coated with mouse anti-GFP (A11120, Life Technology) or mouse anti-Flag (F3165, Sigma) using Dynabeads Antibody Coupling Kit (14311D ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-HA (Monoclonal Anti-HA antibody produced in mouse, H9658 Sigma-Aldrich, 1:1000) and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse anti-FLAG (Anti-FLAG M2 mouse monoclonal antibody, F1804-1 Sigma-Aldrich, 1:1000), mouse anti-HA (Monoclonal Anti-HA antibody produced in mouse ...