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Citations for Millipore Sigma :
1301 - 1350 of 4306 citations for Recombinant Human NTRK2 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... SMGs were digested in 500μl RPMI-1640 containing 5% fetal calf serum (FCS; Sigma Aldrich), 6µl Collagenase-II (23mg/mL ...
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bioRxiv - Biophysics 2019Quote: ... with the addition of 10 mM HEPES and 0.5% fetal calf serum (FCS, Sigma-Aldrich) was used if not stated otherwise ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest) and 1% Penicillin–Streptomycin (PS, Sigma-Aldrich) at 37 °C and 5% CO2 in a humidified incubator.
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bioRxiv - Cell Biology 2020Quote: ... and re-suspended in ice cold 2 % (v/v) FCS/PBS (PAA Laboratories/Sigma Aldrich) following centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in flow cytometry buffer (FB: 2% FCS, 3 nM EDTA (Sigma, E9884) in PBS) ...
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bioRxiv - Immunology 2021Quote: ... 107 BAL cells per mL were suspended in 90% FCS and 10% DMSO (Sigma-Aldrich) and cryopreserved in liquid nitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-MB-231 and BrM cells were cultured in 10% (v/v) FCS (Sigma-Aldrich)-supplemented RPMI-1640 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich Inc.) were used ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO, USA) and 120 µg/ml gentamicin (Refobacin ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant plasmid DNA was transferred to Escherichia coli strain Rosetta 2(DE3)pLysS (Novagen). At least four colonies for each experiment were sequenced to check for errors in the PCR ...
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bioRxiv - Neuroscience 2021Quote: ... Peprotech) and recombinant rat IFNγ (reconstituted in pH 8 sodium phosphate [10mM; Sigma-Aldrich] were diluted to the required concentrations (LTα 1μg + IFNγ 75ng per injection ...
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bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of recombinant PRDX6 was induced with 1mM isopropyl β-d-thiogalactopyranoside (IPTG, Sigma). The culture was incubated for 20h at 18°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant protein was then concentrated using centrifugal filter units (Amicon Ultra-4, Millipore) and dialyzed using Slide-A-Lyzer dialysis cassette into storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... HMWHA or BCM− was pre-incubated with 300 IU/mL recombinant HYAL2 (Sigma-Aldrich) for 30 min prior to addition to co-cultures.
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Digestion was performed overnight at 37 °C using recombinant dimethylated SOLu-trypsin (Sigma-Aldrich) with a trypsin:protein ratio 1:20 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Purified recombinant S proteins were concentrated using Amicon filtration devices (EMD Millipore, Billerica, MA), buffer-exchanged into PBS and analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was purified by affinity chromatography using anti-FLAG affinity resin (Sigma). The purified protein was precipitated by 20% trichloroacetic acid ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RNase R (30 pmols) or Bovine Serum Albumin (BSA, Sigma-Aldrich, MO, USA) were alone or combined with Csm5 WT or Csm5Δ46 (90 ...
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bioRxiv - Molecular Biology 2022Quote: We concentrated recombinant EGFP fusion proteins using Amicon Ultra centrifugal filters (10K MWCO, Millipore) to an appropriate protein concentration in 250 mM NaCl salt buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µM AMP (Serva) and active recombinant AMPK (16 ng) (Sigma-Aldrich; 14-840) in a total volume of 30 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, R and D) and Purmorphamine (1.5 μM, Sigma). Plates were then incubated at 37°C at 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... This basal medium was completed with 5ng/ml recombinant mouse LIF (Sigma ref LIF2050), 3µM CHIR99021 (Sigma ref SML1046-5MG ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... followed by cleavage of the His-tag with 3C protease and dephosphorylation with bovine alkaline phosphatase (Sigma). Further purification was performed by ion exchange chromatography (Source Q for WT and the S897E ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tag was removed by cleavage with 16 U of thrombin (Ref 27-0846-01, Sigma) added directly onto the beads for 2h at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... pneumoniae LicB with an N-terminal 10×His affinity tag in a modified pET-19b vector (Novagen) was overexpressed in E ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...