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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was cleaved by overnight incubation with thrombin (from bovine plasma, Sigma Aldrich) at 4 °C during dialysis ...
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bioRxiv - Biochemistry 2020Quote: ... His-PBP1B containing fractions were pooled and treated with 2 U/mL of thrombin (Novagen) for 20 h at 4 °C during dialysis against dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... and 100 µL of alkaline phosphatase-conjugated anti-HIS tag antibody (Sigma-Aldrich, cat: A5588), diluted 1:2,000 in TBST ...
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bioRxiv - Microbiology 2021Quote: ... PfArlH or biotinylated PfArlI were subsequently mixed with HIS-Select® Nickel Affinity resin (SIGMA) while lysate of cells overexpressing Strep-SaArlI was mixed with Streptactin column material (IBA GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... DTT was added to a final concentration of 0.5 mM and His-SUMO protease (Sigma) was added to a concentration 100-fold lower than the total protein concentration ...
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bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were subjected to purification using either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
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bioRxiv - Cell Biology 2021Quote: ... and a western blot was performed using anti-HIS (Sigma H-1029, mouse, 1:3000) and anti-HA (Biolegend MMS-101R or ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM NaPO4 ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... For super-shift assays monoclonal 0.2 μl of Anti-His antibody (H1029, Sigma-Aldrich, USA) was used ...
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bioRxiv - Plant Biology 2023Quote: ... with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore) following manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was purified by affinity chromatography using Ni-NTA His-bind resin (Novagen). The column packed with the resin was equilibrated with binding buffer ...
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bioRxiv - Cell Biology 2024Quote: ... containing HI-2 at final concentration of 100 µM (or vehicle control DMSO, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich, F7524), Penicillin-Streptomycin 100U/ml (Sigma Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.4 and then bound to poly-prep chromatography columns containing His-bind resin (Novagen). The yeast lysate mixture was loaded in the columns ...
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bioRxiv - Biochemistry 2023Quote: ... and His-AC elution buffer were supplemented with 0.1 v/v% Tween-20 (Sigma Aldrich), 0.1 v/v% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 1 µg/mL His-EqtII-EGFP and DAPI (D9542, SIGMA) at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen®). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic® ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged Arc was immunopurified using 50 µL of anti-FLAG M2 Magnetic Beads (Sigma-Aldrich, M8823) while endogenous TNIK was pulled-down with 10 µL of anti-TNIK antibody (BD Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... coli EF-G (704 aa, C-terminally His6-tagged) cloned into pET24a+ plasmid (Novagen, kanamycin resistance vector) was transformed into an E ...
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bioRxiv - Microbiology 2021Quote: ... FLAG®-tagged MprF was detected both by a mouse anti-FLAG® primary antibody (Sigma-Aldrich) and goat antimouse secondary antibody (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged zebrafish Grk1a or Grk1b was isolated using anti-FLAG M2 Affinity Gel (#A2220) from Millipore-Sigma as described previously (15 ...
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bioRxiv - Biochemistry 2021Quote: ... Flag-tagged H2B was purified by anti-Flag immunoprecipitation with ANTI-FLAG® M2 Affinity Gel (Millipore) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: GST-tagged Af1521 macrodomain was produced in-house and coupled to glutathione Sepharose 4B beads (Sigma-Aldrich), essentially as described previously (Hendriks et al. ...
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bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma, #SAB4301135). Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma ...
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bioRxiv - Genetics 2019Quote: Localization of tagged proteoforms in embryos was visualized by immunohistochemistry using Mouse Anti-FLAG M2 (F1804 Sigma), followed by biotinylated goat anti-Mouse IgG (BA-9200 ...
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bioRxiv - Immunology 2022Quote: ... Cells were treated with EGFP tagged esiRNA that served as an NC (NC-esiRNA-EGFP, Sigma-Aldrich). About ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TBST and incubated with the appropriate HRP-tagged secondary antibody (Sigma-Aldrich) for 3 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... expressing C-terminally 3x Myc-tagged TTP or ZFP36L1 were seeded onto poly-lysine (Sigma-Aldrich, P4832) pretreated glass coverslips for 48 h ...
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bioRxiv - Genetics 2024Quote: ... APOBEC3C tagged with the superFLAG epitope was detected by immunoblotting with an anti-FLAG mouse antibody (Sigma anti-FLAG M2 antibody F3165 ...
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bioRxiv - Plant Biology 2020Quote: ... or 15N-labeled NH4Cl (98 atom % 15N, Sigma-Aldrich, no. 299251), respectively ...
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bioRxiv - Immunology 2020Quote: ... 50 μl of horseradish peroxidase labeled anti-IgY conjugate (Sigma-Aldrich) diluted 1:30,000 was poured into each well ...
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bioRxiv - Developmental Biology 2020Quote: ... The 14C-labeled peptides were transferred onto p81 filter paper (Millipore) and washed three times with 95% ethanol for 5 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: RGCs were labeled with FG (Fluorogold) (Sigma, St. Louis, MO, USA) by injecting the FG solution into the superior colliculi using a stereotaxic device (Stoelting ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were labeled with 5 mM 4-thiouracil (Sigma-Aldrich) for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... 50 µL of horseradish peroxidase-labeled anti-IgY conjugate (Sigma-Aldrich) diluted 1:30,000 was poured into each well ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-labeled goat anti-rabbit (Sigma-Aldrich #A-0545; 1:10000), and goat anti-mouse (Sigma-Aldrich #A-0168 ...
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bioRxiv - Neuroscience 2020Quote: ... GFP was labeled with 1:250 mouse anti-GFP (Sigma-Aldrich, Cat#G6539 ...
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bioRxiv - Cancer Biology 2020Quote: ... Log phase bacterial cultures were labeled using FITC (Cat. F7250, Sigma) as described earlier78 ...
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bioRxiv - Cancer Biology 2020Quote: ... Labeled media was prepared with 10% dialyzed FBS (Sigma-Aldrich, F0392). Cells were then seeded and cultured as described above ...
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bioRxiv - Plant Biology 2021Quote: 15N-labeled KNO3 (99 atom % 15N; Sigma-Aldrich; Cat. No. 335134) or 15N-labeled NH4Cl (98 atom % 15N ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled for 16 h with BrdU (Sigma, 100 μM). At the end of the labeling period ...