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Citations for Millipore Sigma :
1301 - 1350 of 4084 citations for Recombinant Human CD2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Purified recombinant proteins were analyzed by SDS-PAGE and concentrated using stirred ultrafiltration cells (Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant proteins (PRMT5:MEP50 complex (SRP0145-25470) and EZH2 (SRP0379) were purchased from Sigma-Aldrich USA ...
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Plant Biology 2019Quote: ... The transformants were then spotted on SD (-Trp -Leu -His) selection media containing 0.5/1mM 3-Amino-1,2,4-triazole (3-AT; Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
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bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2023Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Cell Biology 2019Quote: Primary human osteoblasts were purchased from Sigma. Co-culture cells were titrated to determine the highest viable seeding density to allow continuous culture for the duration of the osteoclast differentiation assay ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 IU human chorionic gonadotropin (hCG, Sigma) was injected 76 h later (14:00 on day 4 of estrous cycle ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 nM human gastrin I (Sigma-Aldrich), 500 nM A 83-01 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... coated with human plasma fibronectin (Millipore Sigma) diluted to 100 μg/mL in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Human B-lymphoid (Sigma-Aldrich, Buchs Switzerland), 293T Flp-in T-REX (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... 10% human serum (Sigma, St. Louis, MO), 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Developmental Biology 2020Quote: ... and human chorionic gonadotropin (hCG) (Sigma Aldrich). Fertilized oocytes were then transferred into M2 medium (Millipore ...
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bioRxiv - Bioengineering 2019Quote: ... human tenascin-C (Millipore Sigma, Catalog #CC065), and human plasma fibronectin (Millipore Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 µg/mL human transferrin (Sigma-Aldrich), 92 nM FeCl3 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2019Quote: ... Human embryonic kidney (HEK) 293T cells (Sigma) were maintained in DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2020Quote: Human neuroblastoma SH-SY5Y cells (Sigma-Aldrich) were grown in a 1:1 mixture of minimal essential medium (HyClone ...
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bioRxiv - Cancer Biology 2019Quote: ... and Human Adipocyte Magnetic Bead Panel (Millipore), a Bio-Plex 200 plate reader and the Bio-Plex Manager 6.1 software (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-human IgG-peroxidase (Sigma-Aldrich), and mouse monoclonal anti-NeuN (clone A60 ...
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bioRxiv - Immunology 2020Quote: ... human fibrinogen (Sigma-Aldrich, St Louis, MO); CD11b-FITC ...
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bioRxiv - Cancer Biology 2019Quote: ... After incubation with human serum (Sigma-Aldrich) for 15 min ...
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bioRxiv - Bioengineering 2019Quote: ... 12.5 mg human insulin (Sigma I-6634), 100 U/mL Penicillin and 10 µg/mL Streptomycin (GIBCO 15140-122)) ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μg/ml human insulin (Sigma-Aldrich) and 2 % vol/vol B27 (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... using mouse anti-human ZCWPW1 (SIGMA SAB1409478) and mouse anti-polyhistidine (SIGMA H1029) ...
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bioRxiv - Developmental Biology 2021Quote: Mouse monoclonal anti-human αTubulin (Sigma, T5168), 1:3000.
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bioRxiv - Biochemistry 2021Quote: ... and human chorionic gonadotrophin (hCG, EMD Millipore) (9) ...
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bioRxiv - Bioengineering 2020Quote: ... thrombin from human plasma (Millipore Sigma, 605190) was resuspended in cold calcium chloride solution (11.1 mM CaCl2 ...
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bioRxiv - Neuroscience 2020Quote: ... gamma-globulin (human blood, Millipore Sigma, #H7379).
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bioRxiv - Cancer Biology 2020Quote: ... apo-Transferrin (human) (Sigma cat no. T5391) 0.01 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Fibronectin (Sigma-Aldrich, F3648, Rabbit anti–human), Collagen IV (SouthernBiotech ...
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bioRxiv - Synthetic Biology 2021Quote: ... 100µg/ml human apo-transferrin (Sigma; T1147), 100ng/ml sodium selenite (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and 10% human AB serum (Sigma Aldrich) in DMEM supplemented with glutamine (2 mM) ...
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bioRxiv - Microbiology 2021Quote: ... Fn from human plasma (Sigma-Aldrich, F0895) was dissolved in PBS at concentration of 20 µg ml-1 and 100 μl sample was adsorbed on each slide for 1 hour at room temperature.