Labshake search
Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for Integrin alpha 5 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 5% BSA diluted in PBS before being incubated overnight at 4°C with rabbit ɑ-MBNL1 antibody (1:1000, Millipore Sigma, ABE241) or mouse ɑ-RFP antibody (1:1000 ...
-
bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with 0.05% Tween-20 in phosphate buffered saline (PBST) for 3 × 5 min with agitation and incubated with anti-β actin (1:1000 rabbit polyclonal (Sigma Aldrich; A2066) or 1:5000 mouse monoclonal (Sigma Aldrich ...
-
bioRxiv - Biophysics 2023Quote: ... Sections were cut (5 µm) and immunolabeled as described above using a rabbit polyclonal anti-Cx43 antibody (Sigma-Aldrich C6219, St. Louis, MO) followed by goat anti-rabbit Alexa 568 (1:4000 ...
-
bioRxiv - Developmental Biology 2021Quote: ... vorticoides at different developmental stages were incubated in 10-5 M serotonin (5-HT) and 5-hydroxytryptophan (5-HTP) (Sigma-Aldrich, USA) for 3 hours ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
-
bioRxiv - Cell Biology 2019Quote: ... 5 µM Nocodazole and 5 mM Diamide (Sigma-Aldrich). Cells were allowed to equilibrate with the new media for 5 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... 5 μM 5-bromo-2’-deoxyuridine (BrdU, Sigma-Aldrich) was added into the culture medium for 10 h ...
-
bioRxiv - Genomics 2020Quote: ... 5-aza-2’-deoxycytidine (5-aza-dC, Sigma, A3656) was diluted in DMSO at a concentration of 10mM and Abl.1 cells were treated using a concentration range of 10 nM to 20 μM 5-aza-2’-deoxycytidine ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 mM ammonium acetate pH 5 (Sigma-Aldrich 73594), 50% methanol (Honeywell LC230-4) ...
-
bioRxiv - Genetics 2020Quote: For survival to 5-azacytidine (5-azaC, Sigma Aldrich), MG1655 and isogenic derivative (Table 1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 nmol/L 3,3’,5-triiodo-L-thyronine (Sigma), 1 μmol/L dexamethasone (Sigma) ...
-
bioRxiv - Biochemistry 2022Quote: ... 5-(iodoacetamido)fluorescein (5-IAF) was from Sigma-Aldrich. SAM was purchased from New England Biolabs ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 μM S-(5’-Adenosyl)-L-methionine (Sigma A7007), 20 U SUPERase·In (Invitrogen AM2694) ...
-
bioRxiv - Cell Biology 2023Quote: ... 5% glycerol with addition of 5 mM desthiobiotin (Sigma).
-
bioRxiv - Cell Biology 2023Quote: ... Normal Rabbit IgG (12-370, Sigma-aldrich, Rabbit polyclonal), Normal Mouse IgG (12-371 ...
-
bioRxiv - Genetics 2019Quote: ... Expression of Scc1(R180D,268D)-HA was induced 1h after starting alpha-factor treatment by addition of newly-made galactose (Sigma-Aldrich, G0750) (2% final ...
-
bioRxiv - Biochemistry 2020Quote: ... The material was transferred into no-stick microtubes (Alpha Laboratories, cat. LW2410AS) and supplemented with 150 U/ml of Benzonase nuclease (Millipore, cat. 71206). Samples were incubated on a rotating wheel for 30 min at 4°C and centrifuged (16,000g ...
-
bioRxiv - Cell Biology 2022Quote: ... Alpha smooth-muscle actin (αSMA) and collagen I (col1) were stained using monoclonal antibodies (1:1000 diluted, Sigma, A2547 and SAB4200678, respectively). For detection of primary antibodies ...
-
bioRxiv - Biochemistry 2019Quote: ... 1 µL of sample solution was spotted on ground steel MALDI target and mixed with 1 µL of alpha-cyano-4-hydroxy-cinnamic acid (CHCA, Sigma-Aldrich, MO) solution (10 mg/mL CHCA in 50% acetonitrile solution with 0.005% trifluoroacetic acid) ...
-
bioRxiv - Cell Biology 2021Quote: ... membranes were then cut based on size and incubated overnight with the following primary antibodies: 1 μg/mL mouse α-alpha-tubulin (EMD Millipore), 1 μg/mL mouse mAb414 (Biolegend) ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were activated with a cocktail of T-cell activators consisting of 40 ng/ml tumor necrosis factor-alpha (TNF-α; Catalog no. T0157; Sigma-Aldrich), 40 ng/ml phorbol myristate acetate (PMA ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA/DNA and alpha/beta tubulin were tested using cellulose beads with immobilized single and double stranded calf thymus DNA (Sigma Aldrich, USA). 1 µg of PfCoSP was mixed with 500 µl cellulose matrix (1mg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk/TBST for one hour at room temperature and incubated overnight at 4°C with the following primary antibodies diluted in blocking reagent: 1 μg/mL mouse α-alpha-tubulin (DM1A, EMD Millipore), 1 μg/mL rabbit α-LEM-2 (Novus Biologicals) ...
-
bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
-
bioRxiv - Physiology 2023Quote: ... Immunohistochemical (IHC) staining for smooth muscle actin was carried out using anti alpha-Smooth Muscle-Cy3 monoclonal antibody C6198 (Sigma-Aldrich Co.).
-
bioRxiv - Cancer Biology 2023Quote: ... C4-12 cells were cultured in phenol free minimum essential medium (MEM, Fisher #A10488-01) alpha media supplemented with 10% charcoal stripped serum (CSS; Sigma-Aldrich #F6765). Cell lines are maintained at 37°C and 5% CO2 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
-
bioRxiv - Biophysics 2024Quote: ... Triton-X 100 in 1x PBS) for 10 min after which it was incubated with primary antibody solution (anti-alpha-tubulin, #T5168, Sigma-Aldrich, Germany) in IF buffer diluted 1:500 for 1.5 h at room temperature while shaking followed by washing thrice with 1x PBS ...
-
bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
-
bioRxiv - Biochemistry 2021Quote: ... mAbs were concentrated using Amicon Ultra-4 Centrifugal Filter Units with a 50 kDa cut-off (Millipore Sigma Cat # UFC805024) following manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... the supernatants were subjected to immunoprecipitation with anti-FLAG mAb magnetic beads (Catalog No. M8823, Sigma-Aldrich, St. Louis, MO) at 4°C overnight ...
-
bioRxiv - Immunology 2021Quote: ... Germany) were precoated with murine anti-HIS mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
-
bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
-
bioRxiv - Genetics 2021Quote: ... glial fibrillary acidic protein (GFAP; polyclonal 1:1000, ZO334, DAKO-Agilent, Santa Clara, CA, USA; monoclonal 1:1000, MAB 3402, Millipore, Burlington ...
-
bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Purified mAbs were concentrated and buffer changed into PBS using Amicon® Ultra-4 50KDa Centrifugal Filter Units (Millipore Sigma). Purified mAbs in PBS were then filtered using sterile 0.2-μm pore size filter devices (Millipore) ...
-
bioRxiv - Biophysics 2022Quote: ... and 200 μl of (in 10 μg/mg mAb concentration) Papain (Papain from papaya Latex, 89% pure lyophilized powder from Sigma). The mixture was then kept in the incubator shaker at 37 degrees Celsius for 8-10 hours ...
-
bioRxiv - Biochemistry 2023Quote: Mouse monoclonal anti-FLAG M2 (#F3165) and anti-CD95 mAb CH-11 (#05-201) was from sigma-Aldrich (Sigma Aldrich Chimie ...
-
bioRxiv - Microbiology 2023Quote: The subtype of the mAb was IgG1 as determined using a subtype identification kit (Sigma-Aldrich, St. Louis, MO, USA). Non-immunoglobins in the ascites were removed by precipitation with caprylic acid ...
-
bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
-
bioRxiv - Cell Biology 2020Quote: ... The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma, SRP0360) were carried out as described previously (Alvarez-Rodrigo et al. ...
-
bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
-
bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml) and 2i (1μM PD325 ...
-
bioRxiv - Cell Biology 2019Quote: ... growth media was replaced with media containing 50ng/ml BMP-12 (recombinant human, Sigma Aldrich) and 10ng/ml IGF-1 (recombinant human ...
-
bioRxiv - Plant Biology 2019Quote: ... The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore). Protein yield was estimated by measuring absorbance at 595 nm using a spectrophotometer (UV-2600 ...
-
bioRxiv - Neuroscience 2021Quote: ... the astrocytes were stimulated with TNF recombinant (10 ng /ml) (Sigma-Aldrich Corporation, Missouri, EUA) or MCM (300 µl ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
-
bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...