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Citations for Millipore Sigma :
1301 - 1350 of 3645 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Plant Biology 2023Quote: The auto activity of yeast baits on Sc-His due to minimal HIS3 expression was determined by spotting them on Sc-His media containing 0-30 mM 3-Amino-1,2,4-triazole (3-AT) (A8056, Sigma-Aldrich, USA). The minimum concentration of 3-AT that completely inhibits the growth of the bait was considered for the Y1H assay (20mM for pdistal ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Biochemistry 2023Quote: ... A soluble extract was prepared by centrifugation at 14,000 × g for 30 min and applied to Ni-NTA His-bind Resin (Millipore Sigma) equilibrated with binding buffer (50 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Microbiology 2023Quote: ... pET28a-His-DNTTIP1 has been generated by in frame insertion of DNTTIP1 cDNA into BamH1/Xho1 sites of pET28a(+) (Novagen, kindly provided by Dr ...
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bioRxiv - Microbiology 2023Quote: ... 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a fast protein liquid chromatography (FPLC ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by four washes with 200 μl of incubation buffer and incubation with 50 μl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked using 5% nonfat milk and probed with anti-poly-His primary antibody (cat. no. H1029; Sigma-Aldrich) at 1:3,000 dilution or anti- GST primary antibody (cat ...
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bioRxiv - Cell Biology 2024Quote: ... The filtered lysate was then incubated for 30 min with 1 ml of equilibrated HIS-Select HF Ni-affinity gel (Sigma) and loaded onto a gravity flow column ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant supplemented with final 10 mM imidazole was incubated with His-Select Nickel Affinity Gel (P6611, Lot No. 110M5166, SIGMA) for 1.5 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The sample capillaries were then introduced into the sample chamber of the light sheet microscope filled with CO2 independent medium supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich) and 1% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... to shift pH for labeling and were tagged with stable-isotope dimethyl labels comprising regular formaldehyde and cyanoborohydride (28 Da shift, designated “light label”) or deuterated formaldehyde and regular cyanoborohydride (32 Da shift, designated “heavy label”) (all reagents from Sigma-Aldrich). Wild type samples were tagged with light labels and samples from alg2hypo mutant samples were tagged with heavy labels ...
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bioRxiv - Molecular Biology 2019Quote: Doxycycline-inducible YbeY.FLAG-expressing cells were grown in heavy (15N and 13C labelled Arg and Lys containing medium) and light (14N and 12C Arg and Lys (Sigma Aldrich) containing medium ...
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bioRxiv - Genomics 2020Quote: ... overlaid them with one drop (approx. 25μl) of mineral oil (light mineral oil, BioReagent grade for molecular biology, Sigma Aldrich, USA), and stored them at −80°C until WGA ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from 4-day-old dark-grown seedlings plus 3hrs light treatment using the Spectrum Plant Total RNA Kit (Sigma-Aldrich). One microgram of total RNA was treated with DNase I to eliminate genomic DNA and then reverse transcribed using SuperScript III (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... as follows: 4µl of a 4% label (“light”: CH2O, 37% formaldehyde solution, Sigma-Aldrich; “medium”: CD2O, 20% formaldehyde, d2 solution, Sigma-Aldrich; “heavy” ...
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bioRxiv - Neuroscience 2019Quote: ... then labeled with a blend of three FITC-conjugated anti-nucleoprotein monoclonal antibodies (Light Diagnostics Rabies DFA Reagent, EMD Millipore 5100) diluted 1:100 in blocking buffer for 30 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... and combinations of the following lysine and arginine isotopologues: for “light” (“L”)–labeled cells L-arginine (84 mg/L) and L-lysine (146 mg/L)(Sigma-Aldrich); for “medium” (“M”)-labeled cells ...
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bioRxiv - Neuroscience 2021Quote: ... laminar appearance under a light microscope) were manually separated and transferred to a flask coated with poly-HEMA (Sigma #P3932-10G) to prevent adhesion ...
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bioRxiv - Molecular Biology 2019Quote: ... Hap1 cells were grown in heavy (15N and 13C labelled Arg and Lys containing medium) and light (14N and 12C Arg and Lys (Sigma Aldrich) containing medium ...
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bioRxiv - Cancer Biology 2022Quote: ... HRP substrate was applied (Lumi-Light Western Blotting Substrate cat#12015200001 from Sigma Aldrich, Immobilon Crescendo cat#WBLUR0020 from Merck Millipore, SuperSignal West Femto Maximum Sensitivity Substrate cat#34096 from ThermoFisher ...
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3MDR, a microcomputer-controlled visual stimulation device for psychotherapy-like treatments of micebioRxiv - Neuroscience 2022Quote: ... height 20 cm) on a smooth acrylic glass base plate in no-visible-light conditions and scented with 1,4-cineol (Sigma Aldrich, USA). Context C consists of a square chamber (16 cm * 16.5 cm * 25 cm ...
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bioRxiv - Neuroscience 2023Quote: ... Their eyecups were then harvested under dim red light with wavelength peaked at 700 nm and placed in oxygenated Ames’ medium (Sigma-Aldrich) in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... then labeled with a 1:100 dilution of anti-nucleoprotein monoclonal antibody blend (Light Diagnostics Rabies DFA Reagent, Millipore Sigma 5100) as well as a 1:250 dilution of rabbit anti-Cre polyclonal antibody (Millipore Sigma 69050 ...
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bioRxiv - Plant Biology 2023Quote: ... Pectate lyase or polygalacturonase activity can be detected by light pink halos against the darker pink background after staining with 0.05% Ruthenium Red (Sigma-Aldrich, R2751) dye solution (solved in water ...
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bioRxiv - Neuroscience 2024Quote: ... Eyecups were transported to the electrophysiology lab in a light-sealed container filled with freshly oxygenated (95% O2 and 5% CO2) Ames’ medium (Sigma-Aldrich), supplemented with 6 mM D-glucose and buffered with 22 mM NaHCO3 to maintain a pH of 7.4 ...
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bioRxiv - Immunology 2020Quote: ... A 3-fold serial dilution of the sera was performed and HRP goat anti-mouse γ-chain was used (SIGMA, Saint Louis, MO, United States A9044). The result was registered using a microtitration plate reader (Anthos 2010® ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA for reverse transcription polymerase chain reaction (RT-PCR) was isolated from 100 mg of oat leaves using TRI REAGENT® (Sigma-Aldrich, St. Louis, MO, USA) according to the provided protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 1.25 mg/ml human insulin (Sigma #I9278), 1 % Penicillin-Streptomycin (Sigma #P4333 ...
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bioRxiv - Neuroscience 2022Quote: ... human basic fibroblast growth factor (bFGF) (Sigma, cat. #F0291), HEPES 1M (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 ng/mL of human bFGF (Sigma-Aldrich). Cells were incubated at 37 °C in a humidified atmosphere with 5% of CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Normal Human Dermal Fibroblasts (NHDFs) (Sigma-Alrdrich, Buchs, Switzerland), a non-cancerous human skin fibroblast cell line ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10 % heat-inactivated pooled human serum (Sigma) and 100 ng/ml macrophage colony stimulating factor (R&D systems) ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgG (Fab specific) – peroxidase-conjugated antibody (Sigma) diluted 1:5000 in blocking buffer was added to the wells and incubated for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... human Collagen IV from Sigma-Aldrich (St. Louis, MO), recombinant human Tenascin C ...