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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for Cow Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... were resuspended in 4 volumes lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM dithiothreitol, 1 mM ethylenediaminetetraacetic acid, 1:400 Sigma protease inhibitory cocktail P8340 ...
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bioRxiv - Neuroscience 2023Quote: ... CaBP (1:2000, rabbit, CB38, Swant or 1:2000, mouse, #300) and VGLUT2 (1:7000, guinea pig, AB2251, Millipore). The slices were washed 3 times in 1% Triton X-100 in PBS for 5 to 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 containing 1 mg/mL 4-nitrophenyl phosphate disodium salt hexahydrate, Sigma) were added to 20 μL of the conditioned medium ...
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bioRxiv - Cell Biology 2023Quote: ... and lysis buffer (1% Triton-X, 10 nM NEM, 1 mM PMSF, 1 x Protease Inhibitor Cocktail, Sigma-Aldrich) was added ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 containing 1 mg/mL 4-nitrophenyl phosphate disodium salt hexahydrate, Sigma) were added to 20 μL of the conditioned medium ...
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bioRxiv - Biochemistry 2023Quote: ... 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000, anti-UBR4/p600 ab86738, Abcam, 1:5000, anti-FLAG M2, Sigma F1804 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 1.25 g mL−1 of lyophilized lysozyme and 1 U mL−1 of benzonase nuclease (Merck Millipore), and lysed with an EmulsiFlex-B15 cell disruptor (Avestin) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated on ice for 3 min and 2 mL wash buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1 % Tween-20, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Neuroscience 2023Quote: ... or PV antibody (1:500, Cell Signaling D3S8R; SST: 1:100, Millipore MA5-16987; PV: 1:1000, Swant GP72) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with primary antibodies overnight at 4C (DARPP-32 1:200 Abcam ab40801; FoxP1 1:500 Abcam ab16645; GAD65/67 1:500 Millipore AB1511; MAP-2 1:1000 Millipore MAB3418) and then washed three times with PBS before an overnight 4C incubation in secondary antibody (Alexa-fluor rabbit 555 IgG ...
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bioRxiv - Immunology 2022Quote: ... the total DNA methylation signature was evaluated in 100 ng of DNA using the commercial kit (Imprint Methylated DNA Quantification kit, Sigma-Aldrich), according to the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Microbiology 2020Quote: For the measurement of proteasome activity a fluorometric assay kit (Proteasome 20S Activity Assay kit) that measures the chymotrypsin-like protease activity was used following manufactured instructions (Sigma-Aldrich). A total of 100 µg of protein extracts were incubated with LLVY-R110 substrate provided by the kit for 2 h at 30°C in dark ...
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bioRxiv - Microbiology 2022Quote: Aldehyde and D-lactate production in Salmonella strains were measured by the Aldehyde Assay Kit and D-Lactate Assay Kit (Sigma-Aldrich), respectively ...
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bioRxiv - Synthetic Biology 2020Quote: ... the intracellular ATP were measured using the enzymatic assay kit (ATP Bioluminescence Asssay Kit HS II, Sigma No. 11699709001, Roche, Switzerland) after the cell disruption of the corynebacterial strains ...
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bioRxiv - Zoology 2020Quote: ... DNA was extracted from individual cysts or juveniles using the “Sigma Extract-N-Amp Kit (XNAT2)” kit (Sigma, St. Louis, MO) according to the protocol reported by Ma et al ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 100% for 30 minutes each) and then embedded in epoxy embedding medium kit (Epon substitute embedding medium kit; 45359, Sigma Aldrich). For this ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated and converted into cDNA using a commercially available kit (GenElute Mammalian Total RNA Miniprep Kit; Sigma-Aldrich, USA). Transcript levels were determined by real-time quantitative polymerase chain reaction (QuantStudio 5 ...
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bioRxiv - Evolutionary Biology 2024Quote: Random-PCR amplification of the Viral DNA or RNA were respectively achieved with the GenomePlex Complete Whole Genome Amplification (WGA) Kit and the TransPlex Whole Transcriptome Amplification (WTA) Kit (Sigma-Aldrich) according the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... all diluted in a 1:1 mixture of PBT and 3% BSA: mouse anti-α-tubulin (1:500, Sigma T6199), rabbit anti-Spd-2 (1:4.000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% ITS-X supplement (WAKO) and purified BSA (3%, WAKO)) and N2B27 (DMEM/F12/GlutaMax + Neurobasal at 1 : 1 (Sigma), 1 × B27 (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were incubated with the following antibodies against phospho-Yap at s112 (1:1000, 13008, cell signaling), Yap (1:2000, NB110-58358, Novus) and Rabbit anti GAPDH (1:8000, PLA0125, Sigma) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Molecular Biology 2020Quote: ... and then modified with 1:1 volume ratio with 1 mg/ml biotin-5-fluorescein conjugate (Sigma-Aldrich, cat #53608) followed by three washes with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... 1 g L−1 (NH4)2-H-citrate and 1 mL antifoam (Antifoam 204, Sigma-Aldrich, St. Louis, MO, USA). The medium was supplemented with with 2 mL L−1 trace elements solution and (1 M ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell Signalling Technology), ERK (1: 1000, Cell Signalling Technology), p-ERK (1: 1000, Cell Signalling Technology) and GAPDH (1: 5000, Sigma) overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... five flies of each sex and genotype were placed into a vial with 2 ml of 1 X dyed medium (2.5 % yeast, 2.5 % sucrose, 1 % agar and 1 % Brilliant Blue FCF – Sigma Aldrich, USA). After 2 h of feeding ...
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bioRxiv - Neuroscience 2019Quote: ... for the final differentiation in neural differentiating medium (NDM) (Neurobasal 1% NEAA, 1% N2 supplement and 1% P/S) supplemented with 1µM of cAMP (Sigma-Aldrich) and 10 ng/mL of BDNF ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated in 5% horse serum in 0.5% Triton-X TBS for 1 hour before overnight incubation with 1:500 anti-GFP or 48 hours 1:300 anti-UBE3A (3E5 Sigma) and 1 hr 1:500 Alexa-Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... two media containing either commercialized mixed bile salts (0.5 g L−1, 1:1 sodium cholate: sodium deoxycholate, cat# 48305, Sigma-Aldrich) or a primary bile salts composition (0.25 g L−1 sodium cholate and 0.25 g L−1 sodium chenodeoxycholate ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with primary antibodies (rabbit anti-miniSOG 1:500 [Tsien Lab]; guinea pig anti-VGLUT2 1:2000 [Millipore #AB2251]; rabbit anti-TH 1:1000 [Millipore #AB152] ...
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bioRxiv - Biochemistry 2020Quote: ... The full-length MtaLonA were prepared by supplementing the growing medium with 1 g l−1 of 15NH4Cl and 4 g l−1 of 2H7/12C6-glucose in 99.8%-2H2O (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... anti-γH2AX (JBW301, 1:1000), anti-P-Ser15-p53 (1:1000) and anti-ATM Ser1981 (10H11.E12, 1:200) were from Millipore. Anti-P-KAP1Ser824 (1:1000) ...
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bioRxiv - Developmental Biology 2021Quote: ... and then with Theobroma cacao extract fraction 5 (100 µg.mL-1) or (-)-epicatechin (80 µg.mL-1) (Sigma-Aldrich, HW101708-1) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS/0.1% Triton X-100 and incubated with the secondary antibody (1:500) and 1 mg ml−1 DAPI (Sigma, D9564) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... a mixture of primary antibodies in PBS-XD overnight (1 μg/ml rabbit anti-GAD67/65, Frontier; 1:4000 mouse anti-glutamate, ImmunoStar; 1:500 goat anti-ChAT, Millipore), and a mixture of secondary antibodies in PBX-XD for two hours (donkey anti-goat ...
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bioRxiv - Neuroscience 2022Quote: ... 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326; 1:800 chicken anti-GFAP, Millipore AB5541 ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed three times with 1 X TBST and incubated for 1 h with 1:10,000 diluted secondary antibody (Anti-Rabbit IgG peroxidase, Sigma-Aldrich) at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 12565501) with 0.01M phosphate-buffered saline and processed immediately for zonula occludins-1 (ZO-1; 1:500, Millipore Sigma, MABT11) and Bmal1 (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and transfer vector at 1:1:1 molar ratio using the X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich; # 6365787001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-phospho-eIF2α (1:1000, CST), mouse anti-eIF2α (1:1000, CST) or mouse anti-GAPDH (1:20000, Millipore) antibodies ...