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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for 6 chloro 4 methyl 2 phenylimino benzo b thiophen 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The cells were washed in PBS and stained with 5 µg/ml 4′,6-Diamidino-2- phenylindole (DAPI) (D9564-10MG, Sigma-Aldrich) in 1xPBS+0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... cells were washed with PBS and their nuclei were stained with 200 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were washed for 1 h with PBX and stained with 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI) (SIGMA, USA) for 10 min in PBX ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2023Quote: ... nuclei and actin were stained in PBST containing 5 µg/ml 4′,6-diamidino-2– phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) and Alexa Fluor 488 phalloidin (diluted in 1/100 ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was counterstained with DAPI (4′,6-diamidino-2-phenylindole, 10 μg/ml, Cat.No.: D27802, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: Nuclear DNA integrity and gross cell morphology in dPPM was assessed with 4’,6’- diamidino - 2 - phenyl indole (DAPI, Sigma-Aldrich) staining and fluorescence microscopy (Nikon Eclipse Ti ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with PBG blocking buffer containing secondary antibodies and 0.4 μg/ml DAPI (4’,6-diaminido-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed thrice with 1×PBS and incubated for 15 min at RT with 0.1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, Poland). After a final three washes with 1×PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were labeled with 4’,6-Diamidino-2-Phenylindole (DAPI) and slides were mounted using Fluoromount mounting medium (Sigma, cat. # F4680).
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell nuclei were stained with DAPI (4 ’,6-diamidino-2-fenilindol) and slides were mounted using FluorSave™ Reagent (Merck Millipore). The sections were observed and visualized on a Leica DM2500 fluorescent microscope (Leica Microsystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 min with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PBS to label cell nuclei ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Cell Biology 2020Quote: ... and functionalised to an oleophilic state by incubating channels with 1% trichloro(1H,1H,2H,2H-perfluoro-octyl)silane (Sigma-Aldrich, 448931) in HFE-7500 (3M ...
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bioRxiv - Genomics 2020Quote: ... 1-4 PCR wells were combined in a tube and 40 μL of 97% 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich #370533) was added ...
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bioRxiv - Systems Biology 2019Quote: ... Hydrophobic surface treatment of polydimethylsiloxane (PDMS) microfluidic devices was performed by incubating channels with 1% Trichloro(1H,1H,2H,2H-perfluoro-octyl)silane (Sigma-Aldrich, 448931) in Fluorinert FC-40 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... the droplets were de-emulsified by adding 100 µL 20 v/v% 1H,1H,2H,2H-Perfluoro-1-octanol (PFO; Sigma Aldrich, 370533) in HFE-7500 and stained for FACS analysis.
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bioRxiv - Microbiology 2020Quote: ... with a manufacturer-provided polymer FC720 and surface-salinized in a vacuum desiccator overnight with 20 µl Trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma-Aldrich, USA). The microfluidic chips were manufactured via soft-lithography ...
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bioRxiv - Genomics 2022Quote: ... was added to the top of the emulsion followed by 40 μL of 100% 1H,1H,2H,2H-perfluoro-1-octanol (Sigma Aldrich, 370533). The samples were vortexed for 3 seconds ...
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bioRxiv - Immunology 2024Quote: ... and the emulsion was effectively disrupted using 20% (vol/vol) 1H,1H,2H,2H-Perfluoro-1-octanol (PFO) (Sigma-Aldrich, #370533-25G) in HFE-7500 (3M™ Novec™ 7500 Engineered Fluid) ...
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bioRxiv - Biophysics 2023Quote: ... the master SU-8 2050 photoresist was treated with trichloro(1H,1H,2H,2H-perfluorooctyl)silane (448931, Sigma-Aldrich, Saint Louis, MO) in a desiccator for overnight ...
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bioRxiv - Physiology 2020Quote: The reduction of yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Germany) was used to measure cellular metabolic activity as a proxy for cell viability [19,22] ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...
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bioRxiv - Developmental Biology 2021Quote: Larvae at 2-6 dpf were first anesthetized with 0.03 % Ethyl 3-aminobenzoate methane sulfonate salt (Sigma-Aldrich, St. Louis, MO, USA) and pinned onto a Sylgard-filled recording chamber (I-2450 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse Tubulin (clone B-5-1-2 from Sigma, 1:5000), rabbit ZW10 (ab21582 from abcam ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... media supplemented with 2% Fetal Bovine Serum (Sigma, TMS-013-B), 1% L-glutamine (Life Technologies 25030081) ...
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bioRxiv - Microbiology 2020Quote: ... Anti-alpha tubulin mAb (clone B-5-1-2; Sigma-Aldrich) and sheep anti-NGF pAb (EMD Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were then washed 2× with buffer B (Sigma–Aldrich, Duolink), with 1% (v/v ...
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bioRxiv - Genomics 2023Quote: ... mouse α-tubulin (1:10,000, B-5-1-2, Sigma Aldrich). Secondary HRP-conjugated antibodies (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-α-Tubulin (B-5-1-2, Merck Millipore/Sigma) antibodies were purchased ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-α-Tubulin (B-5-1-2, Merck Millipore/Sigma) antibodies were purchased ...
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bioRxiv - Plant Biology 2022Quote: ... DSF supplementation was performed by adding with 5 μM of cis-11-methyl-2-dodecenoic acid (Sigma) to the media.
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bioRxiv - Neuroscience 2020Quote: ... one containing 20% ethanol adulterated with 100 μM (Days 1 and 2) or 250 μM (Day 3) quinine hemisulfate (Sigma-Aldrich, St. Louis, MO) and the other containing water ...
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bioRxiv - Microbiology 2023Quote: ... 2H-perfluoro-1-octanol (Sigma-Aldrich, STL, MO, USA) to collect the capsules ...
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bioRxiv - Immunology 2020Quote: ... 4 or 6 h with staurosporine (5 µg/ml, Sigma) at 37°C ...
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bioRxiv - Genomics 2021Quote: ... containing 4 μl of 6 M Guanidine HCl (GuaHCl, Sigma) as lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... We injected 6-OHDA-hydrobromide (4 mg/ml; Sigma-Aldrich) that was dissolved in saline containing 0.02% ascorbic acid into the right medial forebrain bundle at the coordinates 1.0-mm posterior ...
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bioRxiv - Biophysics 2024Quote: ... and 2 mM Trolox (6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid, 238813, Sigma) was flowed into chamber and then mounted onto microscope stage for smFRET observations ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was then incubated for 2h at 4 °C with 10 μl of anti-FLAG M2 Magnetic beads (Sigma-Aldrich, M8823) pre-washed with TBS ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was incubated 2h at 4°C with ANTI-FLAG antibodies® M2-peroxydase (Sigma-Aldrich, used at 1/ 1000 dilution). The antibodies were detected by using Lumi-Light Western Blotting Substrate (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were clarified by centrifugation at 12000g and incubated 2h at 4°C with pre-equilibrated α-FLAG M2 affinity gel beads (Merck-Sigma, A2220). Beads were washed three times with lysis buffer ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... were incubated for 2h at 4°C with 15 µL anti-FLAG conjugated Sepharose beads (ANTI-FLAG® M2 affinity gel, Sigma, A2220) in 500 µL NENT-100 (20 mM Tris HCl [pH 7.4] ...
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bioRxiv - Molecular Biology 2021Quote: ... X0-3 and X0-4 oligonucleotides (Sigma-Aldrich) in a buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 µM 4-hydroxytamoxifen (OH-Tam, Sigma Aldrich) was added to induce Cre-mediated recombination in the mouse Ctsd gene resulting in a premature stop codon ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 and 4 were custom made from Sigma in the pLKO.1-Puro-CMV-tGFP vector backbone ...