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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for 6 Amino benzooxazole 2 thiol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: Approximately 15 mg of the left TA muscle was homogenized using a glass tissue-homogenizer in 1.5 ml ice-cold lysis buffer (6 M Urea, 2 M Thiourea, 10 mM DTT, Complete Protease Inhibitor (1 tablet per 10 ml lysis, Sigma-Aldrich), PhosSTOP (2 tablet per 10 ml lysis ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining 2 ml were added to falcon tubes containing 4 ml of 6 M GndHCl (Sigma Aldrich, Saint Louis, Missouri) and mixed thoroughly to denature the samples ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed again for three times before the final staining of the nuclei using 4’,6-diamidin-2-phenylindol (DAPI, Sigma-Aldrich). Afterwards ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Neuroscience 2020Quote: ... The cells were centrifuged for 5 min at 700g and treated for 10 min with DAPI (4’,6-diamidino-2-phenylindole) (1:4000, Sigma, 62248) to label dead cells ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then dried in 80% ethanol and mount on a glass slide with antifadent AF1 (Citifluor, USA) added with 4’-6’-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) for counterstain at a final concentration of 2 μg.ml−1 ...
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bioRxiv - Cell Biology 2020Quote: ... C9H2Cl4O2 was purchased from Tebu-Bio and used at 2μM working concentration. Monastrol (Ref. M8515) and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Ref. D8417) were purchased from Sigma-Aldrich. S-Trityl-L-cysteine (STLC) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cryosections were then counterstained (5 min) with 0.5 µg/ml 4’,6’-diamino-2-phenylindole (DAPI; Sigma-Aldrich® Cat#D9542) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently transduced twice with viral supernatant within 48 hrs in the presence of 50 IU/mL IL-2 and 6 mg/mL polybrene (Sigma-Aldrich). TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×104 cells were seeded onto ultra-low adherence 6-well culture plates in 2 mL of serum-free DMEM-F12 media (Sigma-Aldrich), supplemented with 10 µg/mL Putrescine ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 20 minutes at room temperature with DAPI (4',6-diamidino-2-phenylindole; 1:150000, Sigma-Aldrich), mounted on glass slides with Fluoromount-G (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... counter-stained with 4’,6-diamidino-2-phenylindole (DAPI) for 5 minutes at room temperature and mounted with Fluoroshield (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... The cells were washed in PBS and stained with 5 µg/ml 4′,6-Diamidino-2- phenylindole (DAPI) (D9564-10MG, Sigma-Aldrich) in 1xPBS+0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Systems Biology 2022Quote: ... cells were washed with PBS and their nuclei were stained with 200 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Plant Biology 2024Quote: Flowers at position 6 were gently incubated in staining solution (25 µM (2’,7’-dichlorodihydrofluorescein diacetate) (H2DCF-DA) (Sigma-Aldrich, D6883), 50 mM KCL ...
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bioRxiv - Physiology 2024Quote: ... subcutaneous white adipose tissue (sWAT) from male C57BL/6 mice was digested with 1 mg/mL type 2 collagenase (Sigma Aldrich) at 37°C for 40 minutes with shaking ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were washed for 1 h with PBX and stained with 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI) (SIGMA, USA) for 10 min in PBX ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2023Quote: ... nuclei and actin were stained in PBST containing 5 µg/ml 4′,6-diamidino-2– phenylindole dihydrochloride (DAPI; D9542, Sigma-Aldrich) and Alexa Fluor 488 phalloidin (diluted in 1/100 ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was counterstained with DAPI (4′,6-diamidino-2-phenylindole, 10 μg/ml, Cat.No.: D27802, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: Nuclear DNA integrity and gross cell morphology in dPPM was assessed with 4’,6’- diamidino - 2 - phenyl indole (DAPI, Sigma-Aldrich) staining and fluorescence microscopy (Nikon Eclipse Ti ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with PBG blocking buffer containing secondary antibodies and 0.4 μg/ml DAPI (4’,6-diaminido-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed thrice with 1×PBS and incubated for 15 min at RT with 0.1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, Poland). After a final three washes with 1×PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cellular suspensions were obtained from cultures and 1 x 106 million tumour cells were resuspended in 6 μL of 2% PVP (Polivinilpirrolidin, Sigma-Aldrich). Microinjection needles were crafted from borosilicate capillaries (1mm outer diameter x 0,58 mm inner diameter ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich) and 5 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were labeled with 4’,6-Diamidino-2-Phenylindole (DAPI) and slides were mounted using Fluoromount mounting medium (Sigma, cat. # F4680).
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bioRxiv - Pathology 2023Quote: ... and the whole brain of wildtype, heterozygous and homozygous mice (2 months, 6 months, 1 year) with phosphate buffer (PBS) containing 1% Triton-X100 (Sigma-Aldrich) and a Complete EDTA-free cocktail of protease inhibitors (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Immunology 2023Quote: ... virus supernatant was aspirated and preactivated PBMCs (0.5-0.8 x 106/well) in TexMACS medium supplemented with 100 IU/ml IL-2 and 6 ng/ml polybrene (Sigma-Aldrich) were added to each well ...
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bioRxiv - Cell Biology 2023Quote: ... Cell nuclei were stained with DAPI (4 ’,6-diamidino-2-fenilindol) and slides were mounted using FluorSave™ Reagent (Merck Millipore). The sections were observed and visualized on a Leica DM2500 fluorescent microscope (Leica Microsystems) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 1 min with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PBS to label cell nuclei ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... LC-MS grade chemicals Ammonium formate (CAS 540-69-2) and formic acid (CAS 64-18-6) were from Sigma (Germany). Authentic metabolic standards like L-alanine ...
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bioRxiv - Molecular Biology 2024Quote: ... The stained cells were mounted on a coverslip with a mounting medium containing 1 µg/mL 4ʹ,6-diamidino-2-phenylindole (DAPI) dilactate (Sigma, D9564) and 100 µg/mL p-phenylenediamine (PPD ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured in 6-well plates with EGM-2 medium supplemented with 4% dextran (Sigma-Aldrich, St. Louis, MO, 31392). Hemodynamic flows were applied to using a 1o tapered stainless steel motorised cone (UMD-17 (Arcus20 Technology ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed in PBS followed by a nuclear staining with DAPI (4’,6-diamidino-2-fenylindool, 1:1000 in PBS, 32670, Sigma-Aldrich). All sections were mounted with Mowiol and a glass cover slide ...
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bioRxiv - Biochemistry 2021Quote: ... The “light” versions of the SILAC amino acids were obtained from Sigma. For Inducible expression of FLAG-PTP1B ...
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bioRxiv - Immunology 2021Quote: ... it was added the substrate 3-Amino-9-ethylcarbazole (AEC) (Sigma-Aldrich), for 20 minutes ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... and developed with chromogen substrate: 0.03% 3-amino-9-ethylcarbazole (Sigma-Aldrich) and 0.015% H2O2 in 1M sodium acetate ...
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bioRxiv - Molecular Biology 2020Quote: ... binding was visualized using 3-amino-9-ethylcarbazole (AEC) (Sigma-Aldrich, Germany) and slides were counterstained using hematoxylin.
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bioRxiv - Cell Biology 2022Quote: ... alongside the toxic amino acid derivatives 50 mg/L Canavanine (Sigma-Aldrich) and 50 mg/L Thialysine (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 6.7 g/l Yeast Nitrogen Base Without Amino Acids (Sigma-Aldrich). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... and 3’-amino-9-ethyl carbazole (AEC Tablets, Sigma, St. Louis, MO) were further used to develop the plate ...
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bioRxiv - Biochemistry 2019Quote: ... and the amino acids were purchased from Sigma (St. Louis, MO, U.S.A.). IAA (Iodoacetamide) ...