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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The plate was then rinsed with PBS and counter-stained with 4′,6-diamidino-2-phenylindole DAPI (Sigma) for 15 min ...
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bioRxiv - Biophysics 2019Quote: Cholesterol depletion was performed by treating the cells with 3 mM methyl-β-cyclodextrin (Sigma Aldrich, Germany). The cells were incubated for 30 minutes were then measured.
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bioRxiv - Plant Biology 2022Quote: ... 3-day-old seedlings were treated by spraying methyl jasmonate (392707, Millipore Sigma, St Louis, MO, USA). The seedlings were then placed in a sealed transparent plastic container that allowed them to continue to grow for a defined period of time ...
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bioRxiv - Immunology 2019Quote: ... MTT (1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan) and paraformaldehyde were obtained from Sigma Aldrich Co (St Louis ...
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bioRxiv - Genetics 2021Quote: ... was propagated and maintained by transferring shoot segments of 3-4 cm with 1-2 young leaves to fresh one-half Murashige and Skoog (Beijing, China, Sigma-Aldrich). Plantlets were grown at 23 ℃ under a 16 h light/8 h dark cycle with a light intensity of illumination of 5000 lux provided by cool white fluorescent lamp tubes ...
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bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA, E3889, Sigma-Aldrich), 0.1 mM ATP (BP413-25 ...
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bioRxiv - Biochemistry 2022Quote: ... and trans-4-Phenyl-3-buten-2-one (20a) were purchased from Sigma-Aldrich (St. Louis, MO). Ketoisophorone (2a ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and thus 3-[2-(diethylamino)ethyl]-7-hydroxy-4-methylcoumarin (AHMC; Cat. No. 188611, Sigma-Aldrich Corp.) was used instead as previously described for the fluorescence quantification (Donato et al ...
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bioRxiv - Neuroscience 2024Quote: Pharmacological experiments were carried out on PCs during simultaneous somatic and dendritic recordings after 10 minutes of control recording using ACSF with the following drugs: 20 µM 4- (N-ethyl-N-phenylamino)-1,2 dimethyl-6-(methylamino)pyrimidinium chloride (ZD7288) (Sigma-Aldrich), or 1 µM TTX ...
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bioRxiv - Microbiology 2023Quote: ... and used without further purification: 4-Hydroxy-3-methoxycinnamaldehyde (458-36-6, Sigma), 3-ethoxy-4-hydroxybenzaldehyde (121-32-4 ...
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bioRxiv - Genomics 2021Quote: ... after which it was quenched with 5 mM of ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA, Sigma, E3889). The quenched reaction was subsequently centrifuged for 5 minutes at 300 rcf and the supernatant was carefully discarded ...
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bioRxiv - Neuroscience 2022Quote: ... mito-tempol[23] (25 μg, Sanbio) and oligodeoxynucleotide (3 μg/μl day 4, 5 and 6 after carrageenan, Sigma-Aldrich), were performed under light isoflurane anesthesia as described.[8a ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (Sigma-Aldrich, 136149) in [0.1 M Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (Sigma Ref. 10760994001). The control DMRT5 transcript was prepared as previously described [50].
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bioRxiv - Bioengineering 2019Quote: 1 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-NaOH buffer pH 7 (Sigma-Aldrich)
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was determined using the 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, Milan, Italy) according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2020Quote: ... Viability was evaluated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric assay (Sigma, #M5655).
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bioRxiv - Physiology 2022Quote: Cell viability was measured by the colorimetric assay showing reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) as previously described (Denizot and Lang ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich) as described [29].
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bioRxiv - Bioengineering 2020Quote: ... This assay relies on the reduction and conversion of yellow 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) reagent (#M2128, Sigma-Aldrich) into purple formazan salt ...
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bioRxiv - Plant Biology 2022Quote: ... After addition of 10μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) solution (5mg/ml, Sigma-Aldrich, USA) the cells were incubated in darkness for another 4 hrs ...
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bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich (St Louis, MO). Phalloidin-iFluor 594 was purchased from Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (MTT) cell growth kit (CT02, Millipore, MA, USA) was used for measuring cell viability ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Microbiology 2023Quote: The effect of drug treatments on cell viability was assessed using the MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (the MTT powder was obtained from Sigma Aldrich). VTN or Huh-7 cells were seeded the day before treatment at a concentration of 7L×L103 cells per well in DMEM supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mg/ml of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich®, St Louis, MO, USA) was added and incubated at 37°C for 3h ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (Pizzato Scomazzon et al. ...
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bioRxiv - Cell Biology 2021Quote: ... MTs were labeled with rat monoclonal antibody YL 1/2 directed against tyrosinated -tubulin (1:1000, Millipore), or rabbit polyclonal antibody against detyrosinated -tubulin (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Genomics 2024Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Millipore Sigma, H4034-25G), 2.0 mM CaCl2 (Millipore Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... they were incubated with 4 mg/mL of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma-Aldrich, Schnelldorf, Germany) dissolved in 100 mM 2-(N-morpholino ...
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bioRxiv - Biophysics 2020Quote: ... N,N’-methylen-bisacrylamide (BIS, 2%, M1533, Sigma), N,N,N′,N′-Tetramethylethylenediamine (TEMED ...
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bioRxiv - Cell Biology 2023Quote: ... N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (A3281, Sigma, MO, USA); 500nM ZSTK474 (S1072 ...
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bioRxiv - Developmental Biology 2023Quote: ... N-[2-(p-Bromocinnamylamino)ethyl]-5- isoquinolinesulfonamine dihydrochloride (H89, B1427, Sigma-Aldrich), were used in this study.
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bioRxiv - Neuroscience 2020Quote: ... The lesions were made by injecting N-Methyl-D-aspartic acid (10 μg/μL NMDA in 0.9% saline; Sigma, Germany) into the dorsoventral and mediolateral hippocampus at 8 sites bilaterally at a flow rate of 0.15 μL/min (see (Table 1 for coordinates) ...
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bioRxiv - Physiology 2021Quote: ... from the “AngII + L-NAME” group (n=8) were given Nω-nitro-L-arginine methyl ester (L-NAME, Sigma-Aldrich) in drinking water (40 mg/kg/d ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds used in these studies are as follows: N-methyl-D-aspartate (NMDA; 1.5ul of 100mM in PBS; Sigma; M3262), recombinant mouse IL1β (200 ng/dose ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ul of reaction was passed over a Dowex cation exchange resin column (N-methyl D-glucamine form) (217484; Millipore-Sigma) to remove any uninternalized 14C-lysine ...
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bioRxiv - Neuroscience 2023Quote: ... first for 1h with a 4% Triton X-100 (Sigma Aldrich) in PBS to permeabilise and a second time for 1h in 0.5% Triton X-100 1% fish gelatin (CAS #9000-70-8 ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... 6-diamidino −2-phenylindole (DAPI) (1:2000, Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... 2% DMSO and 300 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS; Sigma) dissolved in microtubule stabilization buffer specific for algae (MTB ...
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bioRxiv - Bioengineering 2024Quote: ... BSA (1%) and PBS-T (0.5%) followed by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI) in PBS (1x) (Sigma-Aldrich, St. Louis, MO, 1:1000) for 20 minutes at RT ...
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bioRxiv - Bioengineering 2023Quote: ... the nuclei were stained at room temperature for 15 minuts with 4’,6-diamidino-2-phenylindole (DAPI)(Sigma Aldrich, D9542, 1:500, 1 mg/ml) to yield a blue nucleic fluorescence by binding to nucleic acids.
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bioRxiv - Biochemistry 2023Quote: ... Avicel PH-101, NAD+, NADP+, ATP, MgCl2, cellobiose, 1-Methoxy-5-methylphenazinium methyl sulfate (mPMS, ref M8640) were purchased from Sigma Aldrich. Xylobiose (O-XBI) ...
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bioRxiv - Cancer Biology 2021Quote: 105 HCT116 cells as well as HCT116 cells with acquired oxaliplatin resistance and HCT116 cells with BOLD-100/KP1339 resistance were seeded (N = 4 for each respectively) into 12-well plate (StarLab) with 2 mL McCoy’s 5A medium (Sigma-Aldrich) containing 10% FCS (BioWest ...
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bioRxiv - Microbiology 2022Quote: Sialidase activity was fluorometrically monitored using the substrate 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-Neu5Ac; Sigma-Aldrich). Reactions were performed in a final volume of 50 μL ...