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Citations for Millipore Sigma :
1251 - 1300 of 2560 citations for RhoD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... Peptides were dissolved in a small amount of 0.1% trifluoroacetic acid (302031, Sigma-Aldrich; TFA), followed by purification with the use of ZipTip (MA01821 ...
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bioRxiv - Biochemistry 2019Quote: ... The native proteins without signal peptides were expressed in BL21(DE3) Gold competent cells (Novagen). The gene encoding the LtgA deletion mutant lacking the alpha helix 503(ATAREIAGKIGMD)513 was chemically synthesized by ProteoGenix ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Biochemistry 2020Quote: ... 100 mM NaCl) and eluted with 50 μg/ml (final concentration) of 3xFLAG peptide (Sigma) in TGE ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 40 µl lysis buffer + β-mercaptoethanol and 0.5 mg/mL 3xFLAG-peptide (Sigma) and incubated 30 minutes at 4°C to dissociate proteins bound to the FLAG-beads ...
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bioRxiv - Biochemistry 2020Quote: ... The mRNA-cyclic peptide libraries were eluted with 600 µL of 0.1% DEPC (Millipore Sigma) water containing 1 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: ... The dried peptide films were dissolved in 200 μL Dimethyl sulfoxide (DMSO) >99.9% (Sigma-Aldrich), vortex for 1 min and sonicated for 1 min ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Neuroscience 2020Quote: ... a synthetic opioid peptide with high MOR specificity and clonidine HCl (Sigma, St Louis, MO). These compounds were aliquoted and concentrated using a Speed Vac and stored at −20°C before experimental procedures ...
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bioRxiv - Physiology 2021Quote: ... and 3X FLAG® Peptide (F4799) were obtained from Sigma Aldrich (St-Louis, Missouri, USA). The PKC inhibitor Gö6983 (ab144414 ...
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bioRxiv - Biochemistry 2021Quote: ... The MERS-CoV peptide was labeled with sub-stoichiometric FITC (Sigma-Aldrich, St. Louis, Missouri) in a basic HEPES buffer (pH 8.2 ...
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bioRxiv - Microbiology 2020Quote: ... the sequence encoding mature proteins without the signal peptide was cloned into pET-14b (+) (Novagen) to generate the expression vector pET14b-6xHis-BcCrh1 ...
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bioRxiv - Systems Biology 2020Quote: ... The dried peptides were reconstituted in 60 μL of 0.1% trifluroacetic acid (TFA) (Sigma-Aldrich). Peptides were then fractionated using SCX StageTip method as described earlier [23] ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins were eluted two times with 600 μL 250ug/mL 3xFLAG peptides (SIGMA, F4799) in TBS [50mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: Anti-FLAG-M2 affinity agarose gel and 3X FLAG peptide were obtained from Sigma Aldrich, n-dodecyl β-D-maltoside (DDM ...
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bioRxiv - Biophysics 2020Quote: ... The remaining FLAG beads were incubated with 150 ng/μL 3X FLAG peptide (Millipore-Sigma) in kinase assay buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The remaining FLAG beads were incubated with 150 ng/μL 3X FLAG peptide (Millipore-Sigma) in kinase assay buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). Peptides recovered from in-gel digestion processing were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plasma insulin and plasma c-peptide were measured using radioimmunoassay (EMD Sigma, Cat #: RI-13K) and bead immunoassay (Luminex ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-PaIAP (rabbit, dilution 1:2500, peptide: [H]-KAENQKARFSDVHGC-[OH], Sigma-Genosys, Haverhill, United Kingdom), anti-PaMIC10 (rabbit ...
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). The peptides recovered were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides were dissolved in 80% ACN/5%TFA with 1 M glycolic acid (Sigma-Aldrich) and incubated with 0.6 mg titanium dioxide (TiO2 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal antibodies to anti-calcitonin gene-related peptide (CGRP; 1:500; C7113, Sigma Aldrich), rat monoclonal antibodies to substance P (SP ...
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bioRxiv - Microbiology 2021Quote: ... 100 μl of elution buffer (150 μg/ml 3X-FLAG peptide, Sigma, in Buffer 2) was added and samples were subjected to end-over-end rotation at 4 °C for 30 min ...
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bioRxiv - Biophysics 2020Quote: ... Protein was eluted with Myosin Buffer supplemented with 100 μg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to Storage Buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were eluted with 300 μl of 250 μg/ml 3xFLAG peptide (Sigma, cat#F4799) in IP buffer without NP40 for 30 min at 25°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and the ATAD5-RLC proteins were eluted with 100 µg/mL FLAG-peptide (Sigma Aldrich) in the same buffer ...
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bioRxiv - Bioengineering 2022Quote: ... The concentration of free thiols in all peptides was determined using Ellman’s reagent (Sigma Aldrich).
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bioRxiv - Plant Biology 2020Quote: ... bound complexes were eluted with 100-120 µl of FLAG3x peptide (100 µg / mL)(Sigma) in 0.2% digitonin containing TGS buffer for 40 min at 4°C.
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bioRxiv - Molecular Biology 2019Quote: ... Beads were then eluted twice10 min in 250μl elution buffer (150ng/μl 3xFLAG peptide (Sigma)) in low salt wash buffer supplemented with RNasin) ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were resuspended in 0.1% formic acid and desalted using C18 ZipTips (Millipore, Burlington, MA), and analyzed by LC-MS/MS.
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bioRxiv - Biochemistry 2020Quote: Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Cell Biology 2021Quote: ... Precipitated complexes were washed with lysis buffer and eluted with the FLAG peptide (Sigma, F4799) at a concentration of 400 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptide mixtures were loaded on a solid phase C18 ZipTip (Millipore, Billerica, MA) and washed with 35 μL 0.005% heptafluorobutyric acid/5% FA (vol/vol ...
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bioRxiv - Neuroscience 2021Quote: ... full length human NPY peptide (10 pmol in 1x PBS, Sigma-Aldrich, Cat. No. N5017), anti-CART antibody (1:500 ...
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bioRxiv - Biophysics 2021Quote: Proteins and peptides were labelled at their N-terminus with Fluorescein 5-Isothiocyanate (FITC, Sigma), purified and quantified following a described protocol [45] ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides from both consecutive digestions were recovered by solid phase extraction using C18 ZipTips (Millipore), eluted in 2x7 μl aliquots of 50% MeCN 0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... The bound protein was eluted four times with 250 μL of HA peptide (Sigma-Aldrich) (0,1 mg/ml).
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bioRxiv - Cell Biology 2020Quote: ... Peptides from both consecutive digestions were recovered by solid phase extraction using C18 ZipTips (Millipore), and resuspended in 0.1% formic acid for analysis by LC-MS/MS ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Developmental Biology 2020Quote: ... and then eluted with 200μl of wash buffer containing 0.4mg/ml FLAG peptide (Millipore Sigma). Eluted proteins were mixed with 2xSDS sample buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Puromycin-labeled peptides were identified using the mouse monoclonal antibody 12D10 (EMD Millipore Catalog# MABE343). Relative protein synthesis levels were determined by densitometry analysis of whole lanes.
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bioRxiv - Cell Biology 2021Quote: ... Peptides were eluted from the C18 SPE in 30 µL 50% acetonitrile (ACN, Sigma, 03150391) and 0.1% formic acid (FA ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg of the lyophilized Aβ42 peptide was resuspended in 300 μl of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Sigma-Aldrich). The mixture was sonicated and vortexed thoroughly to ensure proper solvation ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound proteins were neutrally eluted with 50 µL of V5 peptide (V7754-4MG, SIGMA) at 1mg/mL in PBS ...
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bioRxiv - Immunology 2022Quote: ... Bound proteins were eluted by incubating the beads with 200µg/ml 3xFLAG peptide (Sigma- Aldrich) in IPP150 containing 0.02% NP-40 ...
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bioRxiv - Plant Biology 2022Quote: ... Elution of immunoprecipitated proteins was performed using 100 μg/mL c-Myc peptide (Sigma, M2435), on ice for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 0.05% Triton X-100) three times and eluted with 3XFLAG peptide (Sigma-Aldrich, cat# F3290). Elution fraction was then subjected to HA beads ...