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Citations for Millipore Sigma :
1251 - 1300 of 2558 citations for Recombinant Rat Cntfr His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Rats were slowly infused with urethane (0.84 g/kg; 102452447, Sigma) (diluted in saline at 150 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated in 1:1000 rat anti-HA (Sigma) or 1:200 mouse anti-T ...
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bioRxiv - Molecular Biology 2023Quote: ... rat monoclonal anti-HA-Peroxidase high affinity (Roche, Sigma-Aldrich, 12013819001), rabbit polyclonal anti-Actin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and rat anti-tyrosine α-tubulin (1:500, EMD Millipore, MAB1864). Secondary antibodies (all used at 1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Control mice received 250µg of IgG from rat serum (Sigma, 18015) i.p ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pre-coated with rat-tail collagen (30 µg ml-1, Sigma) at a density of 3.0×104 cells/well in glucose-supplemented DMEM medium (containing 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were stained with 1:500 rat anti-HA (Sigma) followed by 1:1000 goat anti-rat Alexa 594 (Thermo) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were stained with 1:500 rat anti-HA (Sigma), followed by 1:1000 goat anti-rat Alexa 594 (Thermo) ...
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bioRxiv - Neuroscience 2023Quote: ... . Rats were anaesthetized with either ketamine (100 mg/kg, i.p., Sigma) and xylazine (10 mg/kg ...
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bioRxiv - Neuroscience 2023Quote: ... a rat monoclonal anti-poly(GR) antibody (clone 5A2, MABN778, Millipore), a Living Colors® EGFP mouse monoclonal antibody (632569 ...
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bioRxiv - Biochemistry 2023Quote: ... HA clone 3F10 (rat monoclonal, conjugated to FITC, Sigma-Aldrich / Merck), GFP (mouse monoclonal ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001 ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used: anti-hemagglutinin (HA, rat, Sigma-Aldrich). For immunoblotting ...
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bioRxiv - Neuroscience 2024Quote: ... the Wistar rats were stereotactically injected with ibotenic acid (Sigma-Aldrich) into the striatum (Fig ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Seeds of the relevant GFP-tagged lines were submerged in either glycerin or in solutions of NaCl (Sigma-Aldrich), mannitol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: Recombinant HA-tagged Gln4 protein for use as a standard was produced by transforming E.coli strain Rosetta-gami B (DE3; Novagen) with pET19b-GLN4 ...
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bioRxiv - Cell Biology 2021Quote: Expression of Flag- and EGFP-tagged proteins was induced with media containing 1 µg/mL doxycycline (Sigma-Aldrich D9891) 12-24 hours prior to harvesting ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates containing FLAG-tagged motors were diluted in assay buffer [AB: P12 buffer (12 mM PIPES (Sigma P-1851), 1 mM EGTA (Sigma E-4378) ...
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bioRxiv - Plant Biology 2021Quote: ... Myc-tagged proteins were captured by addition of 75 μL EZview Red Anti-c-Myc Affinity Gel beads (Sigma). Beads were collected with centrifugation and rinsed at least 3 times with wash buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... coli expressing FLAG-tagged RIN4 protein was incubated with 20 µL of anti-FLAG M2 affinity gel (Sigma-Aldrich) at 4°C for 2 h ...
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bioRxiv - Biophysics 2020Quote: HP1α-AID-sfGFP cells expressing mCherry-tagged HP1αWT or HP1αI165E were treated with control media (no auxin) or 1 mM auxin (NaIAA, Sigma #I5148) for 16 hours before being fixed in 4% paraformaldehyde for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: Poly-histidine (His6)-tagged proteins were expressed and purified by affinity chromatography using Ni-NTA agarose beads (Novagen, USA) according to the manufacturer-recommended instructions ...
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bioRxiv - Microbiology 2020Quote: ... For elution of FLAG-tagged proteins and their complexes 100-300 µL of 0.1 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...
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bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
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bioRxiv - Developmental Biology 2019Quote: ... expression of Flag-tagged UNC-3 in the total cell lysate was detected using mouse anti-Flag (Sigma, #F3165), expression of Flag-tagged UNC-3 in the IP was detected using rabbit anti-Flag (Sigma ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The Strep-tagged MGE proteins were eluted with buffer A supplemented with 2.5 mM desthiobiotin (Novagen and Sigma-Aldrich). The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The Strep-tagged MGE proteins were eluted with buffer A supplemented with 2.5 mM desthiobiotin (Novagen and Sigma-Aldrich). The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen) ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... HA-tagged proteins were immune precipitated for 2.5 hours at 4°C using Ezview anti-HA agarose (Sigma-Aldrich) as described above ...
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bioRxiv - Biophysics 2020Quote: ... the purified mEGFP-tagged NbALFA was concentrated to 1 mg/ml using an Amicon Ultra 30K filter (Merck Millipore) and stored at -80 °C before use ...
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bioRxiv - Biochemistry 2024Quote: ... and expression of the GFP-tagged protein was verified by western blot using an anti-GFP antibody (Sigma-Aldrich). Colonies with the highest expression for a given construct were used to inoculate 300 mL of YEPD media containing 200 µg/mL Zeocin and grown overnight with shaking (220 rpm ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Molecular Biology 2023Quote: ... The various 3xF tagged NRPD1 and CLSY proteins were detected using a monoclonal anti-FLAG-HRP antibody (Sigma #A8592). RDR2-eGFP was detected using an anti-eGFP polyclonal antibody68 ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated using a 30K MWCO concentrator (EMD Millipore) to 0.2 ml and diluted to 0.5 ml in GF150 buffer (25 mM HEPES [pH7.4] ...
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bioRxiv - Cell Biology 2024Quote: ... 3FLAG-6HIS-YFP-tagged proteins expressed from the endogenous locus were immunoprecipitated using anti-FLAG M2 antibodies (F3165, Sigma) (Unnikrishnan et al. ...
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bioRxiv - Biochemistry 2024Quote: ... which contained soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2024Quote: ... which containing soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
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bioRxiv - Genomics 2022Quote: ... SPA-tagged protein was pulled down from clarified lysates with an anti-FLAG antibody (Sigma-Aldrich, #F1804-1MG, RRID:AB_262044) bound to Protein A-Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and analyzed by Western blotting for detection of 3xFLAG-tagged IsdF using an anti-FLAG M2 antibody (Sigma, #F3165) and infrared imaging (Odyssey CLx ...
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bioRxiv - Molecular Biology 2023Quote: The FLAG-tagged peptide and Pgk1 were detected using the anti-FLAG M2 (Sigma; cat# F1365; 1:1000 dilution) and anti-Pgk1 22C5D8 (abcam ...