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Citations for Millipore Sigma :
1251 - 1300 of 3335 citations for PMM2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... and recombinant human epidermal growth factor (EGF; Sigma-Aldrich, E9644) were added to the medium at a concentration of 5 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... human insulin to 10µg/mL (Sigma Aldrich, St. Louis, MO), human epidermal growth factor to 20ng/mL (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 0.005 mg/ml human plasma fibronectin (EMD Millipore) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 ug/ml human insulin (Sigma-Aldrich, Gillingham, UK). D6 ...
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bioRxiv - Cell Biology 2020Quote: ... fibrinogen (human plasma derived) and WKYMVm were from Sigma Aldrich. Recombinant fNLFNYK ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with human rhinovirus 3C protease (8 units/ml; Novagen), and incubated without agitation (4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Human Dermal Fibroblasts were purchased from Sigma (cat # 106-05A). A vial of cells was thawed ...
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bioRxiv - Immunology 2021Quote: ... and human interferon-a 2B (15 ng/mL, Sigma-Aldrich). All cell counts were done manually using a hemocytometer and trypan blue exclusion.
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bioRxiv - Molecular Biology 2021Quote: ... human IPMK cDNA was subcloned into pGEX4T plasmid (Sigma Aldrich), expressed in Escherichia coli ...
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bioRxiv - Biochemistry 2020Quote: Human serum-derived Factor IX was purchased from Sigma Aldrich, MO ...
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bioRxiv - Microbiology 2022Quote: Human Cerebral Microvascular Endothelial cells hCMEC/D3 (obtained from Millipore) were grown in EndoGRO-MV complete media (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse anti-human nuclei clone 235-1 (1:250; Millipore), rabbit anti-histone H3.3K27M mutant (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human type I or type III collagen (Millipore Sigma) were immobilized on a CM5 sensor chip (Cytiva ...
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bioRxiv - Genomics 2020Quote: ... human collagen type III (88 ng per well; Sigma-Aldrich) or a combination of human collagen type I and type III (88 ng of each collagen type per well) ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% (v/v) Human AB serum (Sigma-Aldrich), 1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% (v/v) Human AB serum (Sigma-Aldrich), 1% (v/v)
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bioRxiv - Molecular Biology 2021Quote: AC16 human cardiomyocytes were purchased from EMD Millipore (SSC #109) and cultured in Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Bioengineering 2019Quote: Human Umbilical Vein Endothelial Cells (UVECs) were obtained from Sigma® (200P-05N ...
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bioRxiv - Genetics 2019Quote: ... A rabbit anti-human FAH antibody (Cat. no. HPA041370; Sigma) was added in PBT at 1:200 and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
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bioRxiv - Genomics 2020Quote: ... and/or 0.4μg human GST-tagged PP2A-α (Sigma, SRP5336) in the presence or absence of AZ’5576 or okadaic acid (Abcam ab120375 ...
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bioRxiv - Immunology 2019Quote: ... cells were blocked in 10% human serum (Sigma-Aldrich, H4522) for 10 minutes at 4 °C.
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bioRxiv - Immunology 2019Quote: ... cells were blocked in 10% human serum (Sigma-Aldrich, H4522) for 10 minutes at 4°C prior to antibody staining.
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bioRxiv - Bioengineering 2019Quote: ... 1 U/ml human thrombin (EMD Millipore, Burlington, MA, USA) and 2 μg/ml ciprofloxacin (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 20ng/ml human epidermal growth factor (hEGF) (Sigma, Cat. No.E9644) and allowed to proliferate over one or two passages ...
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bioRxiv - Immunology 2021Quote: ... were coated with either goat anti-human IgG (Sigma #I2136) or IgM (Sigma #I1636 ...
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bioRxiv - Bioengineering 2021Quote: ... and brain-derived neurotrophic factor (BDNF, human recombinant, Sigma-Aldrich). Concentrations used were in the range 50-100 ng/ml ...
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bioRxiv - Molecular Biology 2020Quote: U2OS human cell lines were grown in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... 5 × 10−3 g/L human insulin (Sigma Aldrich, USA), 5 × 10−5 M hydrocortisone (Upjohn Laboratories SERB ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding shRNAs directed against human CDH13 mRNA (Sigma) were cloned into the pTRIPΔU3-EF1α-EGFP lentiviral vector ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-human β1 integrin monoclonal antibody (clone 12G10, Millipore).
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bioRxiv - Cancer Biology 2019Quote: ... Human lung carcinoma PC9 cells were obtained from Sigma-Aldrich, and H1299 cells were provided from ATCC (Manassas ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) prepared at 1:3000 in PBST was then added and plates were incubated for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... Animals were ovulated using human chorionic gonadotropin (Sigma-Aldrich, C1063) according to Sive et al ...