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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated with blocking and permeabilization solution (PBS containing 0.5 % Triton-100X (Sigma-Aldrich) and 3 % bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were diluted in blocking solution supplemented with 0.1% Tween-20 (Sigma Aldrich 274348) and incubated with tissues overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Blocking was performed using a solution of PBS containing 3% bovine serum albumin (BSA; Sigma), 5% horse serum (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The blocking agent 6-Mercapto-1-hexanol and Phosphate-Buffered Saline (PBS) (Sigma-Aldrich Canada) (10.14 mM Na2PO4 ...
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bioRxiv - Bioengineering 2023Quote: ... Grids were washed with PBS and blocked with blocking buffer (0.5% fish skin gelatin (Sigma) and 0.1% BSA-c (Aurion ...
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bioRxiv - Neuroscience 2024Quote: ... EPSCs were isolated by blocking chloride channels with the addition of picrotoxin (75uM; Sigma-Aldrich) into the recording ACSF ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated with blocking buffer (TBS with 0.1% Tween-20 (Sigma-Aldrich, T8787-50ML), 0.5% Triton X-100 ...
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bioRxiv - Physiology 2024Quote: ... and treated with a blocking solution (2% bovine serum albumin in PBT; Sigma-Aldrich #A9647) for 1 hr at 25–27 °C or overnight at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The nephrocytes were blocked with blocking buffer [50 mM Tris-Cl (pH 7.4) (Sigma-Alrich), 0.1% Triton X-100 (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked for 1 hour in blocking buffer [3% Skim milk (Sigma-Aldrich) in T-TBS buffer (0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... Peptide stock solutions were filtered using PVDF 0.22 µm syringe filters (Millipore Milex-HV) before use.
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bioRxiv - Cell Biology 2020Quote: ... an aliquot containing ~ 100 μg of peptides were resuspended in 133 mM HEPES (SIGMA) pH 8.5 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were eluted by adding 100 µl of 200 µg/ml 3xFLAG peptide (Sigma) in TBS and incubating with rotation at 4°C for 30 min.
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bioRxiv - Molecular Biology 2020Quote: ... C-18 ZipTip columns for concentrating and desalting the peptides were purchased from Millipore, Billerica ...
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bioRxiv - Immunology 2021Quote: ... MOG35-55 peptide (WatsonBio Sciences) was emulsified in Complete Freund’s Adjuvant (Sigma-Aldrich F5881) and given as one-time subcutaneous (s.c. ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-EGFP tagged ATP11C was eluted with 0.2 mg/ml Flag peptide (Sigma-Aldrich) in the wash buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Beads were resuspended in 120 μL 2 mg/ml 3X Flag Peptide (Millipore F4799) in Proteinase K buffer and eluted for 30 minutes at 37° C on a thermomixer at 850 RPM ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mg/mL of the peptides were solubilized in 200 μL HFIP (Sigma-Aldrich), vortexed for 1 min and sonicated in 50/60 Hz bath sonicator for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... and peptides were solid-phase-extracted using Sep-Pak Light cartridges (Millipore, Billerica, MA). The peptides were separated by SCX chromatography and were analyzed on a Thermo LCQ Classic instrument (Thermo Electron ...
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bioRxiv - Biochemistry 2020Quote: ... Mass inaccuracies were corrected using ProteoMass Peptide and Protein MALDI-MS calibration standard (Sigma). Glycan compositions were manually inspected using MALDI LIFT-TOF/TOF MS/MS.
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bioRxiv - Neuroscience 2020Quote: ... the complex was eluted using 0.15 mg/ml 3×FLAG peptide (Millipore Cat# F4799) in GDN buffer ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were extracted in 70% ethanol plus 5% formic acid (Merck-Millipore) twice for 20 min with permanent shaking ...
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bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was eluted with 100 μg/ml of 3X FLAG peptide (Sigma-Aldrich) in buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were eluted with 200 μg/ml of 3xFLAG peptide (Millipore Sigma cat #F4799) in TBS supplemented with 10 mM CaCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mg of lyophilized CTD peptides were dissolved in 30 µl DMSO (Sigma-Aldrich). One molar equivalent of Atto-488 NHS ester (Atto-tec ...
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bioRxiv - Molecular Biology 2022Quote: ... washed in Lysis buffer with 225mM salt and Eluted with 3X Flag peptide (Sigma). The eluted fraction was loaded on 4-15% gradient gels with Input and immunoblotted with appropriate antibodies.
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bioRxiv - Neuroscience 2019Quote: ... The peptides were eluted into collection tubes and acidified with formic acid (Sigma Aldrich). Afterwards ...
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bioRxiv - Plant Biology 2021Quote: ... The peptide samples were desalted and purified with C18 ZipTip (Millipore ZipTips, micro-C18). The 1.0 μl of each sample was loaded on MALDI plate with 1.0 μl of the matrix ...
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bioRxiv - Molecular Biology 2020Quote: ... or from anti-Flag resin with buffer containing 250 μg/ml 3xFlag peptide (Sigma). Fractions containing protein were pooled and loaded on a Superdex 200 column preequilibrated in Amylose/Flag buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides are acidified with 10 μl 10 % trifluoroacetic acid (TFA, Sigma-Aldrich, T6508). One Evotip (Evosep ...
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bioRxiv - Molecular Biology 2022Quote: ... The synthesized peptides were dissolved in ultrapure water (Milli-Q system, Millipore, Bedford, MA), store at 4°C for later use.
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bioRxiv - Microbiology 2022Quote: ... The resulting peptides were desalted using C18 ZipTip (Catalog No. ZTC18S096, Millipore, Boston, MA) as indicated previously described [22] ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were eluted from pooled beads with 0.5mg/ml FLAG peptide (#F3290, Millipore Sigma) by shaking at 1000 rpm for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... and immunoprecipitated proteins were eluted 3 times with 150µL 3x-FLAG peptides (Sigma, F4799) for 25 min each at 25°C ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 50 mM biotin were replaced with 200 μg/mL 3x FLAG peptide (Sigma-Aldrich). Samples were buffer-exchanged into wash buffer containing DTT (dilution factor 1:400 ...
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bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted with Wash Buffer containing 0.2 mg/ml 3xFLAG peptide (Sigma). CSB proteins were further purified by gel filtration (Superdex 200 Increase ...
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bioRxiv - Cell Biology 2019Quote: ... the beads were eluted in 30µl 300 µg/ml 3XFLAG peptide (Sigma-Aldrich, #F4799) at 4°C for 45mins ...
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bioRxiv - Immunology 2021Quote: ... pulsed or not with 1 or 10 μg/ml OVA peptide 323–339 (Sigma) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... beads were incubated with 200 μg/ml 3x FLAG peptides (Sigma-Aldrich, F4799-4MG) for 2 hrs at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The reduced peptide was alkylated with 35 μL of 1.5 M iodoacetamide (Sigma-Aldrich) and incubated in the dark at room temperature for 15 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Complexes were eluted with buffer #2 supplemented with 150 ug/ml 3xFLAG peptide (Sigma) for 1 hr at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... peptides were dialyzed with 500 - 1200 MWCO dialysis tubing (Sigma-Aldrich, St. Louis, MO) against DI water ...
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bioRxiv - Bioengineering 2021Quote: ... The crude peptides were the precipitated in ice cold diethyl ether (Et2O; Sigma-Aldrich), collected by centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... the peptides were purified and desalted via ZipTip C18 columns (Millipore, Burlington, MA, USA). The digests were analyzed via nanoLC-MS/MS in a Thermo Scientific Q-Exactive Mass Spectrometer coupled to a nanoHPLC EASY-nLC 1000 (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Peptide was stored at 10 mg/ml in 100% dimethyl sulfoxide (DMSO; Sigma-Aldrich) at −20°C.
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bioRxiv - Molecular Biology 2021Quote: Primary rabbit antibodies used in this study were anti-calmodulin binding peptide (CBP; Millipore), anti-Glucose-6-phosphate dehydrogenase (G6PDH ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used were rabbit anti-calcitonin gene-related peptide (1:5000, Sigma C8198) and rabbit anti-protein gene product 9.5 (1:500 ...
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were activated in vitro with 2 μg of SIINFEKL peptides (Sigma) in the presence of recombinant human IL-2 (50 U/ml ...
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bioRxiv - Biochemistry 2022Quote: ... then eluted in 100 mM elution buffer + 1 mg/ml 3× Flag peptide (Sigma) at 4°C for 30 min.