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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID:AB_177521), Rabbit anti-Homer1 (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... or anti-VSV matrix protein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2347) and subsequently with HRP-conjugated goat anti-mouse IgG (1:750 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rabbit anti-zinc transporter 3 (ZnT3 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins of interest were detected with anti-FLAG antibody (Sigma F1805, 1:1000) and anti-PCNA antibody (Santa Cruz sc-8349 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with addition of 1 µL of 500 mM dithiothreitol (Sigma) at 25°C for 1 h and alkylated with 3 µL of 500 mM iodoacetamide for 1 h at room temperature in the dark and quenched with 2 µL of dithiothreitol for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000; Sigma-Aldrich, catalogue# SAB2500462 ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... UM cells were transduced with virus supplemented with 8 µg/ml polybrene (Sigma Aldrich, Saint Louis, Missouri, USA) and 24 h after transduction ...
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bioRxiv - Cell Biology 2020Quote: ... McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich). After 24 h ...
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bioRxiv - Immunology 2021Quote: ... The swabs were placed into 2ml of virus transport medium (VTM) comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... the cell culture supernatants were pooled to concentrate the virus 20 times using 10,000 MWCO spin columns (Millipore), and aliquots were stored at −70°C [14] ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... P2 virus was then harvested by pelleting cells by centrifugation at 4000g and decanting into 5% FBS (Sigma), and then stored in the dark at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
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bioRxiv - Cell Biology 2022Quote: ... Infectious media containing virus was collected 40 hrs later and filtered with a 0.45μm PES membrane filter (Millipore). Live filtered virus was used to transduce cells with polybrene (10μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Cancer Biology 2022Quote: ... the virus-containing supernatant was centrifuged 500 x g for 5min and filtered with 0.45um PES filters (Millipore), followed by ultracentrifugation (25,000rpm for 2h) ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Genetics 2020Quote: ... 10 μL of each virus was diluted in growth media containing polybrene (10 μg/mL; Sigma-Aldrich, USA) then mixed with cells ...
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bioRxiv - Genomics 2021Quote: ... We filtered and concentrated the virus in PBS using Amicon Ultra-15 centrifugation filters (Millipore, Burlington, MA; #UFC905024). The viral titer was measured with the AAVpro(R ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... The virus was passaged twice in Vero E6 cell line (ATCC) maintained in DMEM High Glucose (Sigma-Aldrich) supplemented with 2% heat-inactivated Fetal Bovine Serum (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Virus-containing supernatant was collected 48-72 hours post-transfection and filtered through 0.45um PES membrane filter (Millipore). For infection ...
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bioRxiv - Immunology 2020Quote: ... and cells were overlaid with virus growth medium containing Avicel microcrystalline cellulose (final concentration of 1.2%) (Sigma-Aldrich). The plates were then incubated at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
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bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated in 1.5 mL virus-containing media supplemented with 0.5 mL fresh media and 0.8 μg/mL polybrene (Millipore Sigma). The 6-well plate was covered with aluminum foil to keep cells in the dark ...
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bioRxiv - Microbiology 2022Quote: ... A27(-) and H3(-) virus stocks were generated in the absence of isopropyl β-d-thiogalactopyranoside (IPTG; Sigma 16758). Plaque forming units /ml and particle counts - calculated from the optical density at 260 nm44 - were determined for each purified recombinant VACV MV stock.
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bioRxiv - Cell Biology 2023Quote: ... Virus containing media was added to ATG3 KO HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich, H9268). After a day ...
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bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
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bioRxiv - Pathology 2023Quote: ... Stock virus was produced after three serial passages in Vero E6 cells using Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 2 % fetal bovine serum ...
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bioRxiv - Microbiology 2023Quote: ... The virus suspension was immediately by passed through a Sephadex G-10 (Sigma-Aldrich, St. Louis, MO, USA) filter column via centrifugation (5 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... of p150 from rubella virus strain RVi/Brooklyn.NY.USA/98/1B CRS (GenBank JN635282; UniProt G3M8F4) was cloned into the pET24a plasmid (Novagen) with a C-terminal hexahistidine purification (His6 ...
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bioRxiv - Immunology 2023Quote: ... virus-infected or HBSS-injected larvae were exposed to either dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO), DMSO-solubilized ramipril (Cayman Chemical Company ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was concentrated to a volume of 0.25 mL in a 100KD cutoff Amicon centrifugal filter (Millipore UFC910096) pretreated with a series of DPBS+ Pluronic F68 (Fisher 24-040-032 ...
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bioRxiv - Cancer Biology 2021Quote: ... CLK2 (1:1000, sigma, HPA055366-100UL), phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50. Beta-Tubulin (1:5000, Sigma Aldrich, #T7816) and Beta-Actin (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were used to detect the following proteins: GFP (1:200, CST, cat. # 2956,), peripherin (1:1000; Novus, cat. # NBP1-05423, NF200 (1:500, Millipore, cat # MAB5266). Primary antibodies were applied and incubated at 4 °C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti-HISTONE H3 (phospho S10 ...
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bioRxiv - Neuroscience 2019Quote: ... Synaptic puncta were identified by the co-localization of pre-synaptic Vesicular Acetylcholine Transporter (VAChT) protein and the post-synaptic Shank protein in MAP2 stained neurons using rb anti-VAChT (Sigma Aldrich; 1:1000), ms anti-shank (Neuromab ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 (mouse mAb clone E-7, Santa Cruz sc-13162; mouse mAB, clone AR12, Millipore UK Ltd ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... and pristane+LPS (single i.p. pristane injection and LPS of E. coli O55:B5, Sigma St Louis ...
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bioRxiv - Cell Biology 2019Quote: ... 13 mm glass coverslips were coated with Poly-D-lysine (Sigma-Aldrich, A-003-E) for 20 min at +4°C ...
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bioRxiv - Bioengineering 2020Quote: ... in Williams E + Cocktail B (see below) with or without 10 μM ketoconazole (Sigma-Aldrich) for the indicated time-points (from 30 minutes to 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Bones were stained with H&E or Tartrate-Resistant Acid Phosphatase (TRAP; Sigma-Aldrich, MO) as indicated ...
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bioRxiv - Neuroscience 2022Quote: ... All plates were previously coated overnight with poly-D-lysine (Sigma–Aldrich, A-003-E). OLs were cultured in DMEM (High glucose-pyruvate ...