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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 2000 cells were plated in 48-well cell culture plates coated with poly-L-lysine (Sigma-Aldrich, P4707). COLO 320HSR were treated with combinations of G007-LK ...
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bioRxiv - Cell Biology 2020Quote: ... are stained for 5 minutes at room temperature RT with 0.5μl of PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling (#P7333, Sigma) and then polished with the exosome spin columns (#4484449 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells growing as described above were washed once with PBS and then incubated with Cell dissociation solution (Sigma) for 20 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ND7/23 cells (a mouse neuroblastoma × rat dorsal root ganglion neuron hybrid cell line; Sigma−Aldrich, ECACC 92090903), were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cancer Biology 2022Quote: Freeze-fracturing of SK-N-SH cells was done as follows: cells were seeded onto collagen-coated (Sigma) sapphire discs in 24-well cell culture plates and differentiated using retinoic acid and BDNF as described above ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... KPCN cell lines were further infected with obtained lentiviruses and desired cells were selected by Puromycin (#P8833, Sigma) treatment (20µg/ml).
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bioRxiv - Genomics 2019Quote: ... cells were dissociated with accutase and seeded at 25,000 cells/cm2 on collagen IV (400ug/mL; Sigma C5533) and fibronectin (100ug/mL ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested at 14-16 hpi cells using accutase solution as per the manufacturer’s instructions (Sigma-Aldrich). ICOSLG surface expression could not be detected by flow cytometry on HFFF-TERTs ...
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bioRxiv - Molecular Biology 2021Quote: Cell lysates prepared from equal amounts of cells (between 100-165 OD600) were fixed with 1% formaldehyde (Sigma) for 15 min at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were passaged or harvested when cells reached 70-80% confluency using Phosphate-Buffered Saline (PBS, Sigma Aldrich) and trypsin (0.25% w/v in versene ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mononuclear cell (MNC) fractions were purified by gradient centrifugation with Biocoll cell separation solution (Merck Millipore, Darmstadt, Germany). Leukemic cells were enriched by negative selection with a combination of CD3- ...
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bioRxiv - Genetics 2020Quote: ... Infected cells grew for 2 days and then the cells were selected with blasticidin (10 μg/mL, Sigma). Infection and selection efficiency were monitored each day using flow cytometry to measure mCherry (BD Accuri C6) ...
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bioRxiv - Genetics 2020Quote: ... Infected cells grew for 3 days and then the cells were selected with blasticidin (10 μg/mL, Sigma). Infection and selection efficiency were monitored each day using flow cytometry to measure mCherry (BD Accuri C6) ...
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bioRxiv - Genetics 2020Quote: ... concentrations of cell suspensions were determined using the Scepter™ 2.0 Handheld Automated Cell Counter (Millipore Sigma, PHCC20060) equipped with 60 uM Scepter™ Cell Counting Sensors (Millipore Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The cell pellet was resuspended at 1×106 cells per ml in freeze medium containing 10% DMSO (Sigma) in 90% FBS (Gibco) ...
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bioRxiv - Immunology 2020Quote: Spleen and lung cell suspensions (150,000 cells/well) were placed in individual wells of ELIspot plates (Millipore-Sigma) that were pre-coated with anti-IFN-γ (AN18 ...
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bioRxiv - Immunology 2020Quote: Spleen and lung cell suspensions (150,000 cells/well) were placed in individual wells of ELIspot plates (Millipore-Sigma) that were pre-coated with anti-IFN-γ (AN18 ...
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bioRxiv - Cancer Biology 2020Quote: ... G16-4 cells as well as BXD-1425-EPN cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma) with 10% fetal bovine serum (Lonza) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were seeded at a density of 250,000 cells/mL in Transwell cell culture inserts with 8μM pores (Sigma). Inserts were placed into wells containing either 700μL culture media ...
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bioRxiv - Genetics 2020Quote: ... The ES cells were plated on mitomycin C treated mouse embryonic fibroblast cells (Millipore Sigma #PMEF-NL-P1) that are G418 resistant ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated cortical cells were plated (50.000 cells/well) in 48-well plates pre-coated with 1.5 μg/mL polyornithine (Sigma). Cells were cultured in a chemically defined Dulbecco’s modified eagle’s/F12 medium free of serum and supplemented with hormones ...
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bioRxiv - Biophysics 2020Quote: A stable cell line was made from MDCKII wild-type cells (ECACC 85011435 purchased from Sigma MTOX1300-1VL) using Lipofectamine 3000 transfection of the Peredox30,69 construct (pcDNA3.1-Peredox-mCherry-NLS ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were split into 15-cm plates at 5,000 cells per plate in 50 µM ganciclovir (Sigma). Surviving colonies were isolated with cloning cylinders and expanded in 50 µM ganciclovir ...
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bioRxiv - Developmental Biology 2022Quote: ... adherent cell cultures cells were washed twice with DPBS then incubated at 37°C in accutase (Sigma-Aldrich) and manually dissociated by pipetting with a P1000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were subjected to ACK Red Blood cell lysis and Ficoll Hypaque (Sigma-Aldrich, St. Louis, MO, USA) gradient separation ...
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bioRxiv - Microbiology 2022Quote: ... the expanded cells were seeded at a density of 50‟000 cells per insert onto collagen-coated (Sigma) 24-well plate inserts with a pore size of 0.4 μm (Greiner Bio-One ...
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bioRxiv - Systems Biology 2022Quote: ... The red blood cells were lysed using Red Blood Cells Lysing solution as per the manufacturer’s instructions (Sigma). The resulting splenocytes were suspended in 1 million per mL (∼ 5mL ...
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bioRxiv - Neuroscience 2022Quote: ... BV2 cells were seeded for the assay at density of 10 000 cells/well on PDL (Sigma, P0899) coated 96-well plates and iMGLs as for cytotox assay ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were plated at 30,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma Aldrich). 48 hrs later ...
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bioRxiv - Systems Biology 2022Quote: The proteins were extracted from the frozen cell pellets by lysing the cells with 1% SDC (Sigma, D6750) in HNN Buffe pH 7.8 (50 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... the stimulated T cells were sorted again for CAR+/TCR-cells and stored in TRI Reagent (Sigma-Aldrich). RNA was isolated using Direct-zol RNA kits (Zymo Research) ...
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bioRxiv - Immunology 2022Quote: ... The rest of the T cells were counted and 10e6 cells were transferred to TRI Reagent (Sigma-Aldrich) for amplicon sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Molecular Biology 2023Quote: ... mCherry positive HEK293 cells were sorted using Sony cell sorter SH800S into 96 well plates containing Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 20% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated at 500,000 cells/mL using the same media on poly-D-lysine (P6407, Sigma‒Aldrich)-coated round coverslips and incubated under standard cell culture conditions for 12 hours ...
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bioRxiv - Immunology 2023Quote: ... 4E7 cells were resuspended in 4 mL NK cell expansion media with 3 uM BX-795 (Sigma-Aldrich), and were left to incubate in a 37°C incubator for 30 minutes ...
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bioRxiv - Pathology 2023Quote: ... wild type mesenchymal cells and epithelial cells of the indicated genotypes were incubated with trypsin/EDTA solution (Sigma) for 5 min at 37°C ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... 293T/17 cells were collected and lysed with Cellytic™ M cell lysis buffer buffer (Sigma-Aldrich, MO) and clarified via centrifugation (1800 x g for 10 min) ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured in ImmunoCult-XV T cell expansion medium supplemented with human serum (10%; Sigma, USA), Penicillin-Streptomycin and IL-2 (100 IU/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Resuspended cells were filtered through 35 μM cell strainer and stained with either propidium iodide (Sigma-Aldrich P4864) or SYTOX deep red stain (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Cell counts and viability were obtained using the Muse cell analyzer (EMD Millipore cat#1000-5175 serial#1846277) and Muse Viability reagent (Millipore cat#MCH600103) ...
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bioRxiv - Immunology 2023Quote: ... LN cells were resuspended in 10mL cell media and plated on 10cm dishes coated with 0.2% gelatin (Sigma). Complete mouse endothelial Cell Medium (EC ...
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bioRxiv - Neuroscience 2023Quote: ... TUNEL-positive cells were stained using the in-situ cell death detection kit TMR red (11684795910; Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in RIPA buffer at about 106 cells in 50 µL (50 mM Tris-HCl (Sigma) pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: Cells and tissues were lysed in cell extraction buffer with protease and phosphatase inhibitor cocktail (P8340, Sigma-Aldrich). Cell lysates were cleared at 20,000g for 10 min at 4°C and protein concentration determined using BioRad protein assay ...
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bioRxiv - Microbiology 2023Quote: ... stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane (Sigma-Aldrich, Saint Louis, Missouri, USA) according to manufacturer’s instructions and added at a concentration of 5×105 monocytes per model the day before adding epithelial cells.
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bioRxiv - Cell Biology 2023Quote: ... Cells that successfully integrated the transduced constructs were selected by treating cells with either puromycin (Sigma-Aldrich Chemistry) or geneticin (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... red blood cells were eliminated by resuspending the pellet in Red Blood Cell Lysis Buffer (Sigma-Aldrich, R7757) at RT for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 70,000 cells of each cell line were seeded on 5 μg/mL Humanplasma Fibronectin (Sigma Aldrich,1mg/mL) in PBS coated (1 hour at 37℃ ...
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bioRxiv - Synthetic Biology 2024Quote: ... with cells solution (OD600 0.01, to reach an average occupancy of 0.5 cells per droplet, in 20% Percoll, Sigma) and substrate solution (0.2 mg/ml polymyxin B (Sigma) ...