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Citations for Millipore Sigma :
1201 - 1250 of 3706 citations for VNN2 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Complement C6 depleted human serum (C1288, Sigma-Aldrich), diluted to final concentration of 2% with dilution buffer (CH50 assay ...
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bioRxiv - Immunology 2024Quote: ... Non-heat inactivated normal human serum (Sigma-Aldrich) was diluted 1:18 (100% ...
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bioRxiv - Biophysics 2024Quote: ... and 5 IU human chorionic gonadotropin (hCG; Millipore) 48 h later ...
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bioRxiv - Immunology 2024Quote: ... 800 ml of human Serum (Sigma Aldrich # H4522) were thawed at 37°C after addition of cOmplete™ ...
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bioRxiv - Neuroscience 2024Quote: ... human citrated plasma (Sigma Cat No. P9523-1ML) was added and time to clot formation was recorded.
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 5% Human serum (Sigma H4522-100ML), 1% Pen/Strep (Thermofisher 15140-122 ...
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bioRxiv - Genomics 2024Quote: ... + 5% Human Serum (Sigma-Aldrich catalogue number H4522) + 1% Penicillin-Streptomycin (Gibco catalogue number 15140122 ...
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bioRxiv - Immunology 2024Quote: Recombinant human TNF-a (rTNF-α; Sigma #H8916) was resuspended in 1X PBS to a stock solution of 0.72ng/nl ...
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bioRxiv - Immunology 2024Quote: ... and endotoxin-free recombinant human Albumin (Sigma A9731) at a final concentration of 5 mg/mL ...
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bioRxiv - Immunology 2024Quote: ... and 5% human heat-inactivated AB serum (Sigma). The antibodies used for stimulation were anti-human CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2024Quote: ... an added positive human IgG Kappa (Sigma-Aldrich) loaded control lane was included (lane P) ...
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bioRxiv - Biophysics 2020Quote: Codon-optimized and his-tagged (C-terminus) genes for HG-series Kemp eliminases (Supplementary Table 1) cloned into the pET-11a vector (Novagen) via NdeI and BamHI were obtained from Genscript ...
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bioRxiv - Cancer Biology 2021Quote: Anti-Flag (Cat. number: F7425) and anti-His (Cat. number: SAB1306085) antibodies were from Sigma-Aldrich (St. Louis, MS, USA). Anti-phospho-γ-H2AX (Ser139 ...
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... Y187 strains were grown on SD/-Trp-Leu medium and transferred to SD/-Trp-Leu-His medium with 10 mM 3-aminotriazole (3-AT, Sigma) in gradient dilutions (1:10 ...
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
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bioRxiv - Biophysics 2021Quote: The bacteriophage T7 gp16 gene was cloned into the pET-16b vector (ampr and 10x-His tag) from Novagen (Germany), inserted between NdeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Biochemistry 2020Quote: ... Trx-His(6)-hSLAP2 or Trx-His(6)-Δlinker-hSLAP2 were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Plant Biology 2021Quote: ... The cell lysate was centrifuged for 30 minutes at 5,000 x g and the supernatant was recovered and mixed with HIS-Select Nickel Affinity Gel (Sigma Aldrich) for protein purification (manufacturer’s instructions were followed) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence was excised with BamHI/EcoRI and ligated downstream of the His tag into the multiple cloning site MCS1 of pETDuet-1 (ampicillin resistance; Novagen), yielding Sll0171-pETDuet-1 ...
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bioRxiv - Biochemistry 2022Quote: ... the protein sequence fused to an N-terminal GB1 solubility tag and a 6x TEV-cleavable His-tag was cloned into pET24b (Novagen). Plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Microbiology 2020Quote: Female BALB/c mice have been injected intraperitoneally with 700 μg recombinant His-tagged TGT protein that was emulsified in complete Freund’s adjuvant (Sigma-Aldrich). Two weeks later ...
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bioRxiv - Cell Biology 2020Quote: ... bacterial pellet was resuspended in 20 ml of cold His-lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0) with protease inhibitor (Sigma-Aldrich), followed by sonication (10 x 20-second pulses) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed 4 x 5 min with TBS and the bound SadP was detected with 1:10000 dilution of anti-His primary antibody (Sigma) and 1:20000 dilution of HRP-labelled secondary rabbit anti-mouse antibody (DakoCytomation) ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Germany) were precoated with murine anti-HIS mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... were equilibrated for >600 sec in 1x PBS prior to loading with his-tagged NiV prefusion F (25 µg/mL in Blocking Buffer (1x PBS, 1% BSA (Sigma)) for 600 sec ...
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bioRxiv - Biophysics 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Microbiology 2019Quote: ... This was followed by four washes with 200 µl of incubation buffer and incubation with 50 µl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at 24°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cerevisiae were cloned into 10×His-MBP-SUMO-nup-SNAP constructs (Figure 2A) via a pET-28a-derived vector (Novagen), and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Biochemistry 2021Quote: ... After removal of the His-tagged MBP by Re-IMAC the unbound material was concentrated in 50 kDa centrifugal concentrators (Millipore) and subjected to SEC on a Superdex 200 Increase column (equilibrated 150 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Microbiology 2019Quote: ... and the PCR product was purified and cloned in-frame with a His-tag into the IPTG-inducible expression plasmid pET-15b (Novagen) to create construct pET15b-Pnf ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was then transferred to a 15 mL tube and incubated with 1 mL His bead slurry (Sigma-Aldrich; P6611) for 20 minutes at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma] ...
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bioRxiv - Biochemistry 2020Quote: ... Samples corresponding to 1.4 OD600 of cells were resolved over SDS-Western blot was developed using anti-His specific antibody (Cat no. Sigma:H1029) at a dilution of 1:3000 and HRP conjugated anti-mouse secondary antibody (Anti mouse IgG-A4416 ...
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bioRxiv - Microbiology 2020Quote: ... was cloned as 10× His-MBP-SUMO-NUP153 (or NSP1)-SNAP constructs (Figure S1) into a pET-28a-derived vector (Novagen) and expressed in BL21-Gold (DE3) ...
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bioRxiv - Molecular Biology 2020Quote: ... Trx-His(6)-Δlinker-hSLAP2 or Trx-His(6)-mSLAP were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...