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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The following antibodies were used: monoclonal mouse-anti-frataxin (1:1000; Milipore Sigma; clone 4F9; MABN2313) and polyclonal rabbit-anti-frataxin (1:1000 ...
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bioRxiv - Biophysics 2024Quote: ... and anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma), revealed with donkey anti-mouse secondary antibodies conjugated to Alexa Fluor 555 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were immunostained with mouse anti-TOMM20 antibody (1:100 dilution, clone 4F3, Sigma-Aldrich #WH0009804M1), rabbit anti-LAMP1 antibody (1:100 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... before staining with primary antibodies mouse anti-human nuclei clone 235-1 (1:250; Millipore, MAB1281) and rabbit anti-microtubule-associated protein 2 (MAP2 ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 1:10000 dilution of anti-mouse alpha-Tubulin (Clone B-5-1-2; SIGMA) for 1 to 3 h at room temperature ...
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bioRxiv - Genomics 2024Quote: ... A mouse monoclonal anti-γ-tubulin (clone GTU-88) antibody (1:5000, Sigma-Aldrich Cat# T5326) was used to probe all blots as a protein loading control ...
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bioRxiv - Biophysics 2024Quote: ... Primary antibodies used were: mouse anti α-tubulin 1:1000 (T6199, clone 6-11B-1, Sigma), rabbit anti Snail [16] 1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... A mouse monoclonal antibody against acetylated tubulin (Sigma-Aldrich, Clone 6-11B-1, diluted 1:500) was used to stain for the ciliary axoneme ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNAs were purified by two rounds of immunoprecipitation with Dynabeads protein G conjugated to anti-BrdU (clone IIB5, Millipore). The purified cDNAs were then ligated using CircLigase II (Lucigen ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane was blocked with 1% milk solution and probed with Anti-6 x His Primary mouse antibody (Millipore #05-949, 1:1000 dilution) overnight at +4°C ...
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bioRxiv - Genetics 2022Quote: ... Chitinase activity was measured by monitoring the hydrolysis of 4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside (Sigma-Aldrich, M5639), a fluorogenic chitin substrate suitable for measuring endochitinase activity ...
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bioRxiv - Bioengineering 2024Quote: ... N-hydroxysuccinimide (NHS) and N-(3-(dimethylamino)-propyl)-N-ethylcarbodiimide hydrochloride (EDC) were bought from Sigma-Aldrich (St. Louis, US), Roswell Park Memorial Institute (RPMI 1640 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 mg/mL SB3-14 (3-(N,N-Dimethylmyristylammonio)propanesulfonate (Sigma T7763)) at 37°C for 1hr and 95°C for 10min ...
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bioRxiv - Bioengineering 2020Quote: ... the water-soluble carbodiimide N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC, Sigma) was added to the premix ...
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bioRxiv - Immunology 2021Quote: ... About 100 mg of N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Sigma-Aldrich) powder was added ...
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bioRxiv - Bioengineering 2021Quote: ... 193 µL of 2 w/w % N,N′-methylenebis(acrylamide) (Sigma-Aldrich) in 1 mM HEPES buffer ...
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bioRxiv - Biophysics 2021Quote: ... and Fmoc-amino acids were activated using N,N’-Diisopropylcarbodiimide (Sigma-Aldrich) and Oxyma Pure (CEM) ...
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bioRxiv - Cell Biology 2020Quote: ... N,N’-Cystaminebisacrylamide (BAC; catalog no. 9809) were purchased from Sigma-Aldrich. Three different batches of sodium acrylate (SA ...
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bioRxiv - Plant Biology 2021Quote: ... samples were derivatized with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 1.0 mg per sample ...
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bioRxiv - Bioengineering 2021Quote: ... N,N-Diisopropylethylamine (DIPEA; 2.23 mL, 12.8 mmol, 4 eq.; Sigma Aldrich) was added to the PEG solution followed by the addition of 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate] (HATU ...
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bioRxiv - Biophysics 2020Quote: ... and 2% (w/v) N,N-methyl-bis-acrylamide (Sigma-Aldrich, M1533) were mixed in PBS on ice and vortexed carefully ...
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bioRxiv - Biochemistry 2022Quote: ... the vehicle consisted of N,N-dimethylacetamide (final volume 0.5%; Sigma-Aldrich) brought to volume with 5% 2-hydroxypropoyl-β-cyclodextrin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15% (w/w) N,N‘-methylenbisacrylamide (Sigma-Aldrich, St. Louis, MO, USA), and 11.7% (w/w ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μl of N-Methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA; 69478, Millipore Sigma) was added and the vial placed in a Benchmark Multi-Therm heat shaker set at 40°C and 250 rpm ...
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bioRxiv - Microbiology 2020Quote: ... was resuspended in 50 μL of anhydrous N,N-dimethylformamide (Sigma-Aldrich) on ice ...
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bioRxiv - Immunology 2021Quote: ... About 20 mg of N-(3-dimethylaminopropyl)-N’- ethylcarbodiimide hydrochloride (Sigma-Aldrich) powder was added at the beginning of the reaction ...
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bioRxiv - Immunology 2021Quote: ... About 100 mg of N-(3-dimethylaminopropyl)- N’-ethylcarbodiimide hydrochloride (Sigma-Aldrich) powder was added ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1 g/100 ml N,N′-methylenebisacrylamide (Sigma-Aldrich, catalog no. M7279), 11.7 g/100 ml Sodium chloride (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The vehicle consisted of N,N-dimethyllacetamide (final volume 0.5%; Sigma-Aldrich) and it was brought to volume with 5% 2-hydroxypropoyl-β-cyclodextrin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 32 µM 5-(N-Ethyl-N-isopropyl)amiloride (EIPA) (Sigma Aldrich, #A3085), 5 mM 1-Heptanol (Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... and N-ethyl-N-sulfopropyl-m-toludine (10 mM, E8506, Sigma–Aldrich) with 3.0 mL of 0.8 M sodium phosphate buffer at pH 6.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 mg/ml N-(3-dimethylaminopropyl)-N′- ethylcarbodiimide hydrochloride (Sigma, 03450) in 50 mM HEPES] for 30 min on slow agitation ...
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bioRxiv - Cell Biology 2022Quote: ... was dissolved to 38.5 mM in N,N-dimethylformamide (Sigma-Aldrich #227056) then combined with the protein solution in a 15:1 (biotinylation reagent ...
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bioRxiv - Cell Biology 2022Quote: ... the amount of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (Sigma, 03449, EDAC) used in the reaction was calculated with the following equation: Carboxylic acid content of beads is provided in mEq/g ...
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bioRxiv - Bioengineering 2023Quote: ... 150 μL of 0.1% n-n-dimethylethylenediamine (cat no. D157805, Millipore Sigma) in 5 M hydrochloric acid was added ...
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bioRxiv - Genomics 2024Quote: ... The final gel composition included 4% N,N-Dimethylacrylamide (Sigma-Aldrich #274135), 6% acrylamide (Sigma-Aldrich #A4058) ...