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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The reactants were dissolved in 25 mL of a solvent mixture consisted of 80% DMSO and 20% tert-amyl alcohol (Sigma Aldrich Co.) in which 1 g of 3 Å molecular sieves was added and the mixture was sonicated for 20 min ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: To extract polar metabolites from mouse (BLA tissue samples, a sample extraction buffer consisting of methyl tert-butyl ether (MTBE, Sigma-Aldrich, 650560), methanol (Carl Roth ...
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bioRxiv - Plant Biology 2024Quote: Gradient tB solution.30%, 50% and 70% Tert-Butanol (tB, Aladdin T119717) in water v/v and 3% w/v Quadrol (Sigma-Aldrich 122262) was added afterwards.
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bioRxiv - Immunology 2024Quote: ... a first extraction targeted at lipid metabolites was performed by adding Methyl tert-butyl ether (MTBE) (Sigma-Aldrich, St. Louis, MO, USA) to a final concentration of MTBE:MeOH (1:4) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were subcultured onto glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5 mM DTT) and concentrated to ∼ 5 mg/mL (Amicon Ultra 4 mL Centrifuge filters, 30 KDa NMWL devices, Millipore Cat# UFC805024). Last ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were incubated for 6 h at 37 °C under a humidified atmosphere with 5% CO2 in the presence of 5 μM carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (Sigma-Aldrich, USA) to induce mitophagy ...
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bioRxiv - Neuroscience 2024Quote: ... cells were subcultured on glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... confluent EnSC monolayers were down-regulated in phenol-free DMEM/F□12 media containing 2% DCC□FBS and decidualized with 10 μM medroxyprogesterone acetate (MPA) and 0.5 mM 8 bromo cAMP (C+M treatment) (Sigma Aldrich). To study the effect of exogenous HA ...
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bioRxiv - Microbiology 2024Quote: ... RNA extraction was performed according to the manufacturer’s protocol except that 1 M 1-Bromo-3-ChloroPropane (BCP) (Sigma-Aldrich) was used instead of chloroform ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transduced with 5 μl (2× 108 IU/μl) viral solution together with 5 μg/ml polybrene (EMD Millipore) for 5-7 days ...
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bioRxiv - Neuroscience 2022Quote: ... 5-16-day old females were fed sheep blood supplemented with 2 mM adenosine 5’-triphosphate (ATP) (Sigma Aldrich, A6419) in aqueous sodium bicarbonate buffer using a new artificial membrane feeder called the blood puck ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions (some of which were pooled from tumors harvested from 2-3 mice) were stimulated for 5 h with PMA (5 ng/ml, Sigma) and ionomycin (500 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were denatured for 5 min at 95 °C in Bolt LDS Sample buffer with 5 % v/v 2-mercaptoethanol (Sigma). 10-30 μg of proteins were loaded in Bolt 4-12 % Bis-Tris gels (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... Cohort 2: 10 (5 male and 5 female) received a single daily injection of BrdU (150 mg/kg, i.p.; Sigma B5002) on days 6 – 8 after infusion and 3 injections of EdU on day 21 after lentivirus injection ...
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bioRxiv - Cell Biology 2022Quote: ... All samples with SDS sample buffer were heated at 95°C for 5 minutes and reduced with 5% 2-mercaptoethanol (Sigma) as needed.
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bioRxiv - Molecular Biology 2023Quote: ... the following doses of chemicals and genotoxic agents were used: 5-Aza-2’-deoxycytidine (5-AzadC; 10 μM, Sigma-Aldrich), 5-Ethynyl-2’-deoxyuridine (EdU ...
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bioRxiv - Biophysics 2023Quote: ... 10 µL of the lipid solution was added to 89.5 µL H2O containing NaCl and 0.5 µL of a 5% solids solution of 2 µm silica microparticles (Sigma Aldrich) to a final concentration of 0.1 mM lipids and 1 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... MICAL1#2 (5′CUCGGUGCUAAGAAGUUCU[dU][dU]3′) (75) and Rab35 (5′GCUCACGAAGAACAGUAAA[dU][dU]3′) (76) were synthetized by Sigma.
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bioRxiv - Cell Biology 2024Quote: ... two siRNA sequences targeting different regions of NOK were designed as #1: 5’-GCAAGAAACAUUCAUGCAU-3’ and #2: 5’-GUCUUUCCCAGGGACACAA-3’ (SASI_Hs01_00043410 and SASI_Hs02_00351937, Sigma, St. Louis, MO, USA). The control siRNA is a scrambled sequence (5’-UUCUCCGAACGUGUCACGUdTdT-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples (5 μg) were used to determine GCase activity upon addition of 10 mM 4-Methylumbelliferyl ß-glucophyranoside (4-MU, Sigma-Aldrich, # M3633) in 1% BSA with or without 2 mM conduritol-b-epoxide (CBE ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde and the slide was mounted using a medium containing 4′,6-diamidino-2-phenylindole-DAPI (Fluoroshield, Sigma-Aldrich, USA). A fluorescence microscopy setup (Leica DM 2000 LED fitted with a Leica DFC450c camera ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 h at 4°C before being rotated overnight at 4°C with Anti-acetyl-Histone H3 (Lys27) (Sigma; 07-360) or Anti-MAFG (GeneTex ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two days at 4°C with secondary antibody along with 0.01 mg/ml 4′-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, Cat#: D9542) to label cell nuclei fluorescently and either 0.2 µl/ml phalloidin-iFluor 594 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... The samples were fixed overnight at 4°C in the mixture of 4% paraformaldehyde (Cat. No. 162-16065, Wako Pure Chemical Industries) and 2% glutaraldehyde (Sigma-Aldrich, G5882) in PBS ...
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bioRxiv - Genomics 2024Quote: ... slides were counterstained in 4’,6-diamidino-2-phenylindole (DAPI) solution and embedded in Mowiol 4–88 mounting medium (Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2020Quote: ... 150,000 cells were stained with 10 μM 4’-(trimethylammonio)-diphenylhexatriene (TMA-DPH) or 2 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine; both obtained from Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 6-Formylindolo[3,2-b]carbazole (FICZ) and 2-Methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) from Sigma-Aldrich. 1-HP ...
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bioRxiv - Systems Biology 2020Quote: ... diluted 1:500 in 1% BSA with 0.3% Triton X-100 for 1.5 h in RT and stained with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, #D9542) for 10 min in RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Mice were kept on 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. No. PHR1731-1G) in drinking water when indicated ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed with PBST and incubated at room temperature for 2 h with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) diluted in PBST ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... for 15 min and blocked for 2 hours at 25 °C or overnight at 4 °C using 2% BSA (Sigma-Aldrich). Samples were incubated with respective primary antibodies at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... were reared in 10g/l of glucose/200µM of 3BDO (3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one) (Sigma Aldrich) supplemented in standard fly food ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were placed in a cylindrical phantom (2 cm diameter, 2 % agarose mixed with 5 % intralipid (Sigma-Aldrich AG, Switzerland). The phantom holder was placed in an imaging chamber filled with water and kept at 28°C.
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bioRxiv - Neuroscience 2023Quote: The mice received three injections every 2 h (200 ul/injection, intraperitoneally in alternative sites of the body) of 5-Chloro-2’-deoxy-Uridine (CldU, Sigma reference C6891 ...
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bioRxiv - Immunology 2024Quote: ... NK cells were pretreated with DMSO or 2-DG (0.5 mM, 1 mM, 2 mM, 5 mM; Sigma-Aldrich, USA), BAY 87 (10 μM ...
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bioRxiv - Neuroscience 2024Quote: ... 5-Ethynyl-2′-deoxyuridine (EdU) injections were administered 2 weeks after BrdU using the same injection protocol (Sigma Aldrich, #900584).
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bioRxiv - Neuroscience 2024Quote: ... one hemisphere of each mouse brain without cerebellum and olfactory bulbs was placed in fluorescence-activated cell sorting (FACS) buffer (1x PBS, 2% FCS and 2 mM EDTA) with 5 μM actinomycinD (ActD; Sigma) and processed for scRNA sequencing as previously described48 ...
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bioRxiv - Biophysics 2020Quote: ... 10 mM NaN3 and 5 mM 2-deoxy-D-glucose (Sigma-Aldrich, Singapore).
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5-aza-2’-deoxycytidine was purchased from Sigma-Aldrich (Cat. A3656, Louis, USA).
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bioRxiv - Cell Biology 2021Quote: ... mouse mAb anti-α-Tubulin (Sigma-Aldrich, clone B-5-1-2, #T5168); mouse mAb anti-Flag M2 (Sigma-Aldrich #F1804) ...
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bioRxiv - Biochemistry 2021Quote: ... Monoclonal anti-alpha-tubulin clone B-5-1-2 antibody (T5168, Sigma-Aldrich) was used as the loading control ...
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bioRxiv - Microbiology 2021Quote: ... hBMECs were subsequently incubated with 5 μM 4,6-diamidino-2-phenylindole (DAPI; Sigma) for 5 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... the DNA methyltransferase inhibitor 5-aza-2 deoxycytidine was from Sigma-Aldrich (A3656), Polyethylenglycol 300 (PEG300 ...
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bioRxiv - Cancer Biology 2021Quote: ... or 3-(1,3-Benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde (KNK437, Sigma Aldrich) for 24 hours prior to collection of cells for RNA extraction.
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bioRxiv - Molecular Biology 2020Quote: ... and the cysteine bonds reduced with 5 mM tris(2-carboxyethyl)phosphine (Sigma) (37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse α-Tubulin (Sigma, B-5-1-2, # T5168, used at 1:5000), mouse α-FLAG (Sigma ...