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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for 4 Nitro n propan 2 yl aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... sections were counterstained with 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) and mounted on slides with VECTASHIELD Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... DAPI (4′, 6′-diamidino-2-phenylindol) was obtained from Sigma (St. Louis, MO). North American genotype PRRSV strain PA8 was propagated in MARC-145 cells and used for this study ...
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bioRxiv - Biochemistry 2022Quote: ... 300 µL cold (0-4 °C) milliQ water containing 2 µg norvaline (Sigma) as an internal standard was pipetted to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-5 µm slices were individually stained with Hematoxylin (Sigma #517-28-2), Picrosirius Red (Abcam #ab150681) ...
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bioRxiv - Cell Biology 2023Quote: ... and 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, #32670-5MG-F), all diluted 1:500 in blocking solution ...
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bioRxiv - Genetics 2023Quote: ... incubated with 4′,6-Diamidino-2-phenylindole dihydrochloride (1:10,000; Millipore Sigma, D8417) in wash buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and cell nuclei were stained with 4’,6-diamidino-2-Phenylindole (DAPI, Sigma) to visualize cell layers and borders of the hippocampus ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:500 dilution + 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) at a 1:1000 dilution.
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bioRxiv - Immunology 2024Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich). Rehydrated slides were also stained for Serum Amyloid A 1-3 after formic acid (70% formic acid in d.d ...
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bioRxiv - Systems Biology 2024Quote: ... we added 750 µl of 2 × 10−4 M nFMLP (Sigma-Aldrich, #F3506) to one well of the second electrophoresis block to obtain a working peptide concentration of 2 × 10−6 M ...
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bioRxiv - Genetics 2024Quote: ... 2 μL of supernatant was mixed with 4 μL triacylglyceride reagent (Sigma, T2449) and 6 μL PBST ...
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bioRxiv - Cell Biology 2024Quote: ... and 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI, 1/3000, Sigma-Aldrich, UK) for 1 hour at room temperature protected from ambient light ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, D9542) for 5 minutes and washed 3-4 times in PBS before being mounted in Mowiol mounting media (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent stain 4’,6-diamidino-2-phenylindole (DAPI) was purchased from Sigma-Aldrich. Revert™ 700 Total Protein Stain and IRDye® 800 CW goat anti-mouse secondary antibody for Western Immunoblotting were purchased from LI-COR Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and 25 mM (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Sigma-Aldrich; H0887) in a humidified 5% carbon dioxide atmosphere at 37 °C 15 ...
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bioRxiv - Immunology 2024Quote: ... stained for 5min with 1μg/ml 4′,6-diamidino-2-phenylindole (DAPI, Sigma) and mounted with ProLongTM Gold (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Coverslips were then fixed with 4% paraformaldehyde (PFA; Booster) with 2% sucrose (Sigma) in 1X PBS for 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, D9542, Sigma-Aldrich. MO.USA) for 5 min.
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bioRxiv - Bioengineering 2024Quote: ... 5-norbornene- 2-carboxylic acid (0.78 ml, 6.4 mmol, 4 eq.; Sigma-Aldrich) was added to the solution and the reaction was stirred overnight at room temperature (RT ...
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bioRxiv - Neuroscience 2024Quote: ... all from Thermo Fisher Scientific] and 4′,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, #D9564) diluted in PBS for 105 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) for 5 min at RT in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... 4 kDa FITC-dextran and 2 MDa FITC-Dextran were from Sigma-Aldrich. 70 kDa TRITC-dextran was from Chondrex Inc ...
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bioRxiv - Biochemistry 2024Quote: ... and HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) were purchased from Millipore Sigma. Texas Red-DHPE (1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% normal donkey serum in PBS with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at room temperature or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma] ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Biophysics 2020Quote: ... pooled and concentrated to 2-4 mg/mL using Amicon Ultra-4 (100 kDa molecular weight cut off) concentrator tubes (EMD Millipore), flash frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Biophysics 2020Quote: ... MEFs transfected with LIM-protein plasmids were plated in 4-well Strex chambers (STB-CH-4W, 2 cm2 × 4 chambers) coated with 50 μg/mL fibronectin (Millipore, FC010), and allowed to recover overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Cell Biology 2021Quote: ... 50 mg/mL EDC and NHS solutions were prepared in 25 mM cold 2-(N-morpholino) ethanesulfonic acid (MES, cat. num. M3671-50G, Sigma), at pH5 ...
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bioRxiv - Microbiology 2022Quote: ... BHI was supplemented with BC (n-alkyl dimethyl benzyl ammonium chloride, Sigma-Aldrich, St. Louis, MO, CAS: 63449-41-2) to achieve appropriate BC target concentrations ...
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bioRxiv - Neuroscience 2020Quote: Apoptotic cells were revealed in 2-days-old live embryos by adding the Acridine Orange vital dye (N-4638; Sigma) to the embryo water at a final concentration of 5 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... allele-specific PCRs were performed on 2 µl crude buccal DNA in a total of 10 µl using the REDExtract-N- AmpTM PCR ready MixTM (Sigma) according to the manufacturer’s protocol using 0.5 µM forward ...
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bioRxiv - Neuroscience 2021Quote: ... partial 5-HT2A/2CR agonist), and 8-hydroxy-2-(di-n-propylamino)tetraline (8-OH-DPAT, 5-HT1A agonist) were obtained from Sigma/Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole, pH 8.0) to 2 mg/mL, and 0.6% n-Dodecyl β-D-maltoside (DDM, w/v) (Sigma-Aldrich) and 0.01% lysophosphatidylcholine (lyso-PC ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse NPCs from E13.5 WT and PlyA-KI forebrain were dissociated and cultured in serum-free N-2/B-27/DMEM medium with 10 ng/ml FGF2 (Life Tech) in poly-L-lysine (PLL, Sigma)-coated 6 well culture plates (Costar ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Neuroscience 2023Quote: ... or acidic pH 6.5 media (26mM NaHCO3, 20mM 2-(N-morpholino) ethanesulfonic acid (MES)(Millipore Sigma, St. Louis, MO, USA), 5mM HEPES ...
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bioRxiv - Bioengineering 2024Quote: ... A 1 wt% alginate solution was prepared in 0.1 M 2-(N-morpholino)ethanesulfonic acid buffer (MES, Sigma Aldrich, M8250). Sulfonated N-hydroxysuccinimide (NHS ...
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bioRxiv - Microbiology 2024Quote: ... the pellets were resuspended in a solution of HEPES 10 mM pH 7.4 and N-lauroylsarcosine sodium salt 2% (sarcosyl) (Sigma-Aldrich) and were incubated on a roller shaker for 60 minutes at RT ...
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bioRxiv - Biophysics 2024Quote: ... The diluted bead solution was dialyzed into 100 mM 2-(N-morpholino)ethanesulfonic acid (MES, 150x volume, Millipore Sigma M3671) at pH 6.0 for 2.5 hours ...
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bioRxiv - Biochemistry 2020Quote: ... 5-(N-Ethyl-N-isopropyl)amiloride was obtained from Sigma-Aldrich (St. Louis, MO). ESOM sodium salt was obtained from Apexbio (Houston ...
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bioRxiv - Microbiology 2021Quote: ... weighted and then transferred to a vial containing 0.5 ml N,N-Dimethylformamide (Sigma), to release labeled fixed carbon from the tissue to the liquid ...
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bioRxiv - Cancer Biology 2020Quote: ... and 25μL of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-butyldimethylchlorosilane (Sigma 375934). Following derivatization ...