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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Tetratricopeptide repeat protein 14 TTC14 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Solution containing primary antibodies for FLAG-tagged proteins are made by adding Anti-FLAG M2 antibodies (produced in mouse) from SIGMA into 10mL of the blocking solution (1:10000) ...
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bioRxiv - Microbiology 2024Quote: ... Solution containing primary antibodies for HA-tagged proteins are made by adding Anti-HA HA-7 antibodies (produced in mouse) from SIGMA into 10mL of the blocking solution (1:20000) ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Neuroscience 2020Quote: ... the blots were incubated with secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-human Fab IgG (H+L) (Sigma, A0293) diluted 1:10000 in blocking buffer for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... After 2 h at RT plates were washed three times and incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Pathology 2021Quote: ... appropriate secondary antibodies were used at 1/5000 and signals were detected using a chemiluminescence system (Immobilon Western HRP Chemilumiscent substrates, Millipore).
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bioRxiv - Plant Biology 2021Quote: ... PVDF membranes were blocked with 5% milk followed by an incubation with first antibodies for REM1.2/1.3 followed by 3 washes and an incubation with second α-rabbit antibody fused to HRP against rabbit (Sigma). Clarity Western 701 ECL (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed two times and horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Sigma Aldrich, St. Louis, MO) was used at a 1:5,000 dilution and incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated with the primary antibody at 1:1,000 in 1x TBST buffer for 1hr followed by 3 × washes for 5 minutes each in TBST buffer and if necessary with a secondary horseradish-peroxidase (HRP) antibody conjugate (Sigma) diluted 1:1,000 in 1x TBST for 1hr followed by 3 × washes for 5 minutes each with TBST buffer ...
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bioRxiv - Microbiology 2021Quote: ... with PBS +0.05% Tween20 and bound FLAG-ACE-2 was detected with HRP-conjugated anti-FLAG antibody (1:20,000, Sigma cat# A8592) and peroxidase substrate (KPL ...
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bioRxiv - Biophysics 2020Quote: ... at 37 °C for 50 min followed by western blot with monoclonal Anti-FLAG M2-Peroxidase (HRP) antibody (Sigma, A8592) for the CDH1-derived nascent chains (1:10,000 dilution) ...
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bioRxiv - Molecular Biology 2022Quote: The following antibodies were used for Western blotting at the indicated dilution: anti-FLAG - HRP conjugate (Sigma M2, 1:10000); anti-Brd8 (Bethyl A300-219A ...
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bioRxiv - Immunology 2022Quote: ... and Goat Anti-Human IgM Fc5μ Fragment specific HRP conjugated secondary antibody for IgM (Cat# AP114P, EMD Millipore, Burlington, MA). After the incubation ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then incubated for 1 h with secondary antibody a-mouse HRP conjugate 1:10000 (Cell Signalling) and chemiluminescent substrate ultra (Millipore) was applied for 5 min before camera detection in Sapphire Biomolecular Imager (Azure Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... wells were washed three times using H2O 0.05% Tween20 and binding of NMDAR antibodies to the immobilized N1-N2B-Fc was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour with a species-appropriate HRP-conjugated secondary antibody (diluted 1:5000 in TBST) before bands were visualised using Immobilon Forte Western HRP Substrate (Merck Millipore). Primary antibodies used were mouse anti-αSMA (Dako ...
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bioRxiv - Pathology 2020Quote: ... and methyl cytosine levels assessed by a specific primary and secondary HRP-conjugated antibody system following the manufacturer’s instructions (Imprint® Methylated DNA Quantification Kit-Sigma). Following development with TMB ...
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bioRxiv - Microbiology 2021Quote: ... ScAb or mAb samples were incubated with the antigen in doubling dilutions and the binding was detected using anti-Human C Kappa horseradish peroxidase (HRP) conjugated secondary antibody (Sigma) (for scAbs ...
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bioRxiv - Synthetic Biology 2020Quote: ... The membrane was then washed several times with Tris-Buffered Saline with 0.1% Tween 20 (TBST) before a 30 in incubation with monoclonal Mouse Anti-FLAG M2-Peroxidase (HRP) antibodies (Sigma-Aldrich) diluted 1:5000 in TBST ...
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bioRxiv - Immunology 2021Quote: ... plates were again washed thrice followed by incubation with 1:5,000 dilution of horse radish peroxidase (HRP) conjugated anti-guinea pig IgG secondary antibody (Sigma-Aldrich) or 1:2,000 dilution of HRP conjugated anti-mouse IgG secondary antibody (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: HRP-conjugated secondary antibodies for Western blotting: goat anti-mouse IgG (Cat# A 4416, Sigma-Aldrich, St. Louis, MO, USA) and goat anti-rabbit IgG (Cat# GtxRb-003-EHRPX ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... An α-mouse IgG conjugated to horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:4,000 (Sigma-Aldrich). To assess cell wall fractionation a mouse α-protein A (Spa ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblot analysis was performed with the antibodies indicated and visualization was achieved with the Immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Microbiology 2020Quote: ... diluted 1:1000 in phosphate-buffered saline (PBS) containing 0.05% Tween 20 (PBS-T) followed by anti-rabbit horseradish peroxidase (HRP)-coupled antibody (Sigma-Aldrich) diluted 1:1000 in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... C1q fixation was detected with rabbit anti-C1q antibodies (1/2000; in-house) and a goat anti-rabbit-HRP (1/2500; Millipore). TMB liquid substrate (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used for immunoprecipitation assay and/or immunoblotting: a mouse anti-Flag and a mouse anti-Flag-HRP antibody (Sigma), a rabbit anti-N antiserum (70) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... washed and incubated with HRP-conjugated goat anti-rabbit secondary antibody and developed using enhanced chemiluminescence substrate (Millipore, ON, Canada). Membrane was stripped using 0.1 N NaOH and reprobed with rabbit anti-beta actin antibody (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with HRP-conjugated goat anti-rabbit antibody (1:10,000, Sigma-Aldrich, St. Louis, MO, USA). The signal was visualized by chemiluminescent detection using ECL.
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903, Sigma) at 1:5,000 dilution at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was washed 3X with 1x TBST for 5 min each followed by incubation with anti-rat IgG secondary antibody conjugated with horseradish peroxidase (HRP) (Sigma) (1:2000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:10000 in Intercept Blocking Buffer for 1.5 hour at room temperature or HRP-conjugate antibodies goat anti-rabbit IgG (A0545, Sigma-Aldrich) and anti-mouse IgG (A4416 ...
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bioRxiv - Microbiology 2023Quote: ... and probed with 1:1000 anti-MntS (rabbit polyclonal) followed by 1:10,000 goat anti-rabbit-AzureSpectra800 (Azure) or 1:1000 anti-FLAG M2-HRP antibody (Sigma-Aldrich) in 3% milk-TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed six times with TBST buffer and blotted with horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies (Sigma). After washing with TBST buffer again ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with secondary-HRP conjugated antibodies (1:10.000; Anti Rabbit IgG HRPO, Millipore BI2407; Anti Mouse IgG HRPO, Millipore BI2413C) for 1h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... HRPA-1::AID*::TEV::FLAG was detected using Monoclonal Anti-FLAG M2-Peroxidase (HRP) antibody (A8592-2MG) from Sigma-Aldrich at a 1:1000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 1% milk-PBST). Aldolase was detected with anti-aldolase-HRP (Abcam ab38905 ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes previously incubated with anti-RplA were incubated with the secondary antibody anti-rabbit IgG-HRP (#A8275, 1:10000, Sigma) for 1 h at room temperature and then washed three times with T-PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween-20 (TBS-T) and incubated for one hour at RT with Mouse anti-FLAG (M2) antibody-HRP conjugated (Sigma) diluted 1:4000 in blocking milk ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (anti-rabbit HRP, DAKO, P0448; anti-chicken IgG-peroxidase, Sigma; anti-mouse IgG peroxidase, A3682, Sigma) were visualized using Amersham ECL or ECL Prime 225 reagent (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad ...
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bioRxiv - Microbiology 2024Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 5% milk-PBST). Anti-ATS and anti-NapL were detected as previously described (Nacer et al ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes probed with HRP-conjugated secondary antibody were developed a 3,3’,5,5’-teramethylbenzidine (TMB) substrate (Genscript L002V or Millipore Sigma T0565). Developed membranes were scanned and then processed with ImageJ.
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bioRxiv - Pathology 2024Quote: ... overnight at 4°C and subsequently incubated with horseradish peroxidase (HRP) conjugated secondary antibodies which were detected by enhanced chemiluminescence (Cat# WBKLS0500; Millipore). Immunoblots were analyzed using ImageLab software (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... This was followed by four washes with 200 μl of incubation buffer and incubation with 50 μl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).