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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Lassa Fever Virus GP1 Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... the ACE2-Fc expression vector was transfected into Expi293T cells according to the manufacturer’s instructions (Sigma-Aldrich; L3287). Five days after transfection the supernatant was collected by first spinning cultures at 300 g for 5 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Spinal cord specimens were then placed in glass tubes filled with Fluorinert (FC-770, Milipore Sigma, Darmstadt, Germany) for imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
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bioRxiv - Microbiology 2020Quote: ... We detected bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody (Sigma Aldrich catalog number A0170).
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bioRxiv - Neuroscience 2020Quote: ... Medium supplemented with the exosome-depleted FCS and antibiotics was subsequently filtered through a 0.22 and a 0.1 µM filter-sterilization device (Millipore). For EV isolation ...
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bioRxiv - Bioengineering 2020Quote: ... Tumor tissue was dissected into fragments of 1-2mm3 and frozen in 90% fetal calf serum (FCS, Sigma) and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... Medium supplemented with EV-depleted FCS and antibiotics was subsequently filtered using 0.22 and a 0.1 µM filter-sterilization devices (Millipore). For EV isolation ...
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bioRxiv - Immunology 2019Quote: ... Monocytes were cultured in RPMI-1640 medium supplemented with 10% FCS and 1% sodium pyruvate (all from Sigma) in ultra-low attachment 6-well plates ...
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bioRxiv - Immunology 2019Quote: ... cells were counted at d0 and 0.4x106 cells/ml were seeded in differentiation medium (RPMI supplemented with 2.5 % FCS, 0.5% dimethylformamide (DMF, D4551, Sigma), L-glutamine and antibiotics) ...
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bioRxiv - Molecular Biology 2021Quote: Wildtype and ΔMETTL3 mES cells were maintained in DMEM supplemented with 15% FCS (ES-009-B, Millipore Sigma), 1X non-essential amino acids (11-140-050 ...
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bioRxiv - Genetics 2021Quote: ... They were transferred to digestion medium (HBSS with Ca2+ Mg2+, FCS 2%, DNase I 0.5mg/mL (Sigma-Aldrich) and collagenase IV 0.5mg/mL (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... The EV-containing eluates were pooled and supplemented with 10% heat-inactivated fetal calf serum (FCS; Sigma-Aldrich), or 0.1% BSA (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and resuspended in PBS containing 0.5 % FCS and subjected to FACS analysis (Guava EasyCyte, Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were visualised on Licor Odyssey Fc (Cambridge, UK) using enhanced chemiluminescence (ECL, Merck Millipore, Darmstadt, Germany). β-actin was used a loading control.
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bioRxiv - Immunology 2023Quote: ... Surface staining was performed for 30 min at 4 °C in PBS supplemented with 2% FCS (Sigma-Aldrich) and 0.01% azide (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted single cells were collected in ADF plus 10% FCS and 9 µM Rock inhibitor (Y-27632, Sigma) to prevent anoikis ...
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bioRxiv - Genetics 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Capricorn, FBS-12A) and 1% penicillin/streptomycin (P/S, Sigma, P0781). The cultured fibroblasts were incubated at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2024Quote: ... Serum-cultured cells were grown on 0.1% gelatin-coated plates in Serum ES media (GMEM, 10% FCS (Sigma), 2mM Glutamax ...
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bioRxiv - Genomics 2024Quote: ... 20% FCS (Autogen Bioclear, Calne, UK) and 1,000 U/ml of leukemia inhibitory factor (ESGRO-LIF, Chemicon/Millipore). For the Ring1B conditional deletion ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Biochemistry 2020Quote: Mouse anti-FLAG M1 antibody and mouse anti-Sulfotyrosine antibody (Sigma) were used to detect the purified wild type C5aR and C5aRΔTyr with Y11F and F14F mutations respectively in the western blotting assays.
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-β-actin (Sigma, mouse monoclonal antibody, 1:5000). After incubation with HRP-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal mouse anti-mouse GFAP (1:2000; Sigma G-A-5), and monoclonal rat anti-mouse IL-6 (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-NeuN (mouse monoclonal, 1:400, Millipore; catalog #MAB377, RRID:AB_2298772). This step was followed by incubation with secondary antibodies for 2h at RT to visualize primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse anti-β-actin (Sigma, mouse monoclonal antibody, 1:5000). After incubation with HRP-conjugated secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Mouse Monoclonal anti-mouse Glutamin Synthetase (GS-6, 1:250, Millipore), Rabbit Polyclonal anti-SDS (PA5-58704) ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Immunology 2023Quote: ... and immunoprecipitated with protein A and protein G sepharose beads (Millipore Sigma). After thoroughly washing beads with SLBA buffer (0.15M NaCl ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...
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bioRxiv - Neuroscience 2020Quote: ... Tau protein ladder (Sigma) was used in some experiments as a control ...
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bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
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bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
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bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...