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Citations for Millipore Sigma :
1151 - 1200 of 4028 citations for Human MOG His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Commercially-available human FMO1 (Sigma, F4928-1VL), bacterially-purified human OSGIN1-6xHis or bacterially-purified C ...
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bioRxiv - Neuroscience 2024Quote: ... anti-human TMEM119 (1:100, Sigma, #HPA051870), anti-mouse Cd68 (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-human β-actin (Sigma, #A5441). Immunoblots were analyzed using horseradish peroxidase secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... along with 5% human AB serum (Sigma) (32–35) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 5% human serum (Sigma-Aldrich) and 5% mouse serum (Sigma-Aldrich)] was added ...
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bioRxiv - Cell Biology 2024Quote: ... human recombinant Insulin solution 5µg/mL (Sigma), M-CSF 100ng/mL (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Human recombinant insulin (Millipore Sigma, Cat#19278), GAD65 (Diamyd Medical ...
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bioRxiv - Microbiology 2024Quote: ... Human serum albumin (0.1 µg, Sigma Aldrich) was added to all samples to control the digestion efficiency ...
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bioRxiv - Biophysics 2020Quote: ... expressing vimentin with a GFP tag were produced by lentiviral transfection (LentiBrite GFP-Vimentin Lentiviral Biosensor, Merck Millipore) of HeLa cells ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cell aliquots were pelleted at 600xg in a swinging bucket rotor centrifuge and washed twice with 200 μL CUT&Tag wash buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine (Sigma); 1x Protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by cleavage of the GST tag by 15 units/ml TEV protease (Sigma T4455-1KU) in PBS buffer overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Strains with degradation tags were treated with 250 µM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) in DMSO and 50 mM potassium phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... was expressed in COS-7 cells and immobilized to anti-T7 tag-antibody-conjugated agarose beads (EMD Millipore) (Matsui et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Blood levels of TAGs were measured by a colorimetric assay using the Triglyceride determination kit (Sigma-Aldrich, #MAK266). LCN2 plasma levels were determined by ELISA (R&D Systems ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen). The fractions containing MGE protein were pooled and concentrated to 1 mL by using a centrifugal concentrator (Sartorius ...
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bioRxiv - Molecular Biology 2021Quote: ... tags and TEV protease were removed by an additional round of affinity chromatography on Ni-NTA-agarose (Sigma). As a final step gel filtration on a Superdex 75 column in 20 mM phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was subjected to Western blot analysis using a polyclonal antibody against the 6Histidine tag (Sigma-Aldrich). After incubation with the second anti-mouse antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: The Cleavage Under Targets and Tagmentation (CUT&Tag) experiments were performed using FLAG antibodies (Flag, Sigma, F1804/F3165) in 3× Flag-POGZ restoring Pogz−/− mESCs by the Yingzi and Frasergen Companies (Wuhan ...
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 (human embryonic kidney) and U2OS (human bone osteosarcoma) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10 % FBS ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T) and human fibroblast cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich), supplemented with fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Biochemistry 2024Quote: Human C-reactive protein (Cat. No. C4063) and Human erythrocyte catalase (Cat. No. C3556) were obtained from Sigma Aldrich chemicals ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 3×106 co-transformants was initially screened for growth on synthetic defined media (SD)-Leu-/ Trp-/ Ura-/ His- media containing 20mM of 3-amino-1,2,4-triazole (3-AT, Sigma-Aldrich). Plasmids were isolated from the potential positive colonies ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Neuroscience 2019Quote: ... Adult zebrafish were assayed for response to a mixture of 8 amino acids (Ala, Cys, His, Lys, Met, Phe, Trp, and Val; 0.1 mM each in distilled water; Sigma). No stimulation was provided for the first 5 mins following which the amino acid mix was introduced in one side of the tank at the rate of 1 ml/min using a syringe pump ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+ -NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The culture media for the dividing cells (HI-10) consisted of RPMI-1640 medium + 10% heat inactivated FBS (Sigma), 100 μg/mL penicillin and 100 μg/mL streptomycin ...
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Immunology 2024Quote: ... whereas CoMiX-FHR4 molecules and VHH controls were detected with a mouse anti-His HRP-conjugated mAb (Sigma-Aldrich). The plate was revealed with OPD/H2O2 HRP-chromogenic mix substrate and the absorbance was measured at 492 nm ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered through a 0.22 µm filter then applied to a 5 mL His-TrapTM HP (Sigma) column pre-equilibrated with the same buffer at a flow rate of 1.5 mL.min-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression and native purification was performed by using His-select Nickel affinity gel resin column (Sigma Aldrich, USA) according to the manufacture’s instruction ...
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bioRxiv - Biophysics 2023Quote: His-FUS LC was labeled with Atto-488 or Atto-647 NHS-ester fluorescent dyes (ATTO-TEC, Sigma-Alrich). Labeling occurred at or near the N-terminus because only the N-terminus and a lysine at residue position 5 in the leader sequence preceding the N-terminal region hexa-histidine tag were expected to react with the NHS-functionalized dye ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), incubated at room temperature for 20mins and poured into a 1-cm separation column (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentrations of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported 21 ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana were tandem affinity-purified using a His-Bind resin followed by anti-Flag-M2-agarose beads (Sigma-Aldrich) as described32 with slight modifications ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Cancer Biology 2020Quote: Human neutrophils were obtained from fresh human peripheral blood from healthy volunteers and were isolated using a Percoll (Sigma-Aldrich) gradient.
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...