Labshake search
Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Glioma pathogenesis related protein 1 GliPR1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... and mouse anti-glial fibrillary acidic protein (GFAP; 1:500, #G3893; Sigma-Aldrich). The next day sections were washed 3 times with the blocking buffer (15 min each ...
-
bioRxiv - Neuroscience 2022Quote: ... The expression of neuronal marker protein NeuN (anti-NeuN, 1:200, MAB377, Millipore) was determined to identify neuronal tdTomato expression.
-
bioRxiv - Neuroscience 2022Quote: ... 10 μg protein was combined with 1 μL 10% Triton X-100 (Sigma) and brought to a volume of 33 μL in Vance buffer (225 mM D-mannitol ...
-
bioRxiv - Bioengineering 2022Quote: ... 1 mM purified protein was mixed with 25% ammonium persulfate (APS) (Sigma-Aldrich) and 6.67 mM [Ru(II)(bpy)3]2+ (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-glial-fibrillary acidic protein (GFAP) (Millipore Sigma, AB5804, 1:500) on shaker ...
-
bioRxiv - Developmental Biology 2023Quote: ... for 1 hr at 115 V before transferring to protein membrane (IPV00010, Millipore) at 50 V for 2 hr ...
-
bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
-
bioRxiv - Cell Biology 2024Quote: ... goat anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000; Sigma-Aldrich, catalogue# SAB2500462 ...
-
bioRxiv - Neuroscience 2023Quote: ... Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID:AB_177521), Rabbit anti-Homer1 (1:1,000 ...
-
bioRxiv - Immunology 2024Quote: ... or anti-VSV matrix protein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2347) and subsequently with HRP-conjugated goat anti-mouse IgG (1:750 dilution ...
-
bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
-
bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
-
bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rabbit anti-zinc transporter 3 (ZnT3 ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins of interest were detected with anti-FLAG antibody (Sigma F1805, 1:1000) and anti-PCNA antibody (Santa Cruz sc-8349 ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with addition of 1 µL of 500 mM dithiothreitol (Sigma) at 25°C for 1 h and alkylated with 3 µL of 500 mM iodoacetamide for 1 h at room temperature in the dark and quenched with 2 µL of dithiothreitol for 10 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... CLK2 (1:1000, sigma, HPA055366-100UL), phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50. Beta-Tubulin (1:5000, Sigma Aldrich, #T7816) and Beta-Actin (1:5000 ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were used to detect the following proteins: GFP (1:200, CST, cat. # 2956,), peripherin (1:1000; Novus, cat. # NBP1-05423, NF200 (1:500, Millipore, cat # MAB5266). Primary antibodies were applied and incubated at 4 °C overnight ...
-
bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
-
bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti-HISTONE H3 (phospho S10 ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
-
bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
-
bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
-
bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
-
bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
-
bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
-
bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
-
bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
-
bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
-
bioRxiv - Neuroscience 2019Quote: ... Synaptic puncta were identified by the co-localization of pre-synaptic Vesicular Acetylcholine Transporter (VAChT) protein and the post-synaptic Shank protein in MAP2 stained neurons using rb anti-VAChT (Sigma Aldrich; 1:1000), ms anti-shank (Neuromab ...
-
bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
-
bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
-
bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
-
bioRxiv - Neuroscience 2019Quote: ... The protein sample was incubated with taxol-stabilized microtubules (15 mg mL-1) and 1 mM AMP-PNP (Sigma) and the mixture overlaid on a glycerol cushion (BRB80 ...
-
bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Sigma) or 16O light (Fisher ...
-
bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
-
bioRxiv - Cancer Biology 2021Quote: ... after which slides were washed in phosphate-buffered saline and stained against Human Nuclei (MAB4190, 1:250, Millipore) and Human specific Ki67 (MAB4383 ...
-
bioRxiv - Pathology 2020Quote: ... and immunostained with human monoclonal anti-CRP antibody (C1688; Sigma-Aldrich, Saint Louis, MO, USA; 1:400 dilution) which specifically detect the 24-kD monomeric CRP epitope[31 ...
-
bioRxiv - Biochemistry 2020Quote: DNA sequences containing human SUN2 were cloned into a modified bicistronic ampicillin resistant pETDuet-1 vector (EMD Millipore), superfolder GFP-KASH fusions (sfGFP ...
-
bioRxiv - Microbiology 2021Quote: ... HMEC-1 cells (human microvascular endothelial cells RRID:CVCL_0307) were grown in HMEC media (MCDB 131 media (Sigma, M8537) supplemented with 10% FBS (HyClone ...
-
bioRxiv - Immunology 2022Quote: Human THP-1 monocytes were differentiated into macrophages by 24 hr incubation with 100 nM PMA (Sigma-Aldrich) in complete RPMI medium at 1.6 × 105 cells/well in 12-well plates ...
-
bioRxiv - Immunology 2022Quote: ... Human Umbilical Vein Endothelial Cells (HUVEC) were grown in 1% gelatin coated wells in M199 medium (Sigma-Aldrich) containing 20% fetal bovine serum (FBS) ...
-
bioRxiv - Biophysics 2021Quote: ... Samples were stained using a 1:100 dilution of the primary antibodies (rabbit polyclonal anti-human IRSp53, Sigma and rabbit polyclonal anti-human IRTKS antibody ...
-
bioRxiv - Immunology 2021Quote: ... Cells were cultured in Human Plasma-like Media (HPLM) (8) + 1% pen/strep + 5% dialyzed serum (Sigma Aldrich). For SARS-CoV-2 peptide stimulation ...