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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-FLAG (Sigma), rabbit anti-CCDC47 (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-FLAG (Sigma). Secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-FLAG (Millipore-Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-Gapdh (Millipore), and rabbit anti-neuron-specific enolase (Polysciences) ...
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bioRxiv - Microbiology 2024Quote: ... Mouse IgG (Sigma-Aldrich) was used as a standard for the determination of total IgG ...
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bioRxiv - Synthetic Biology 2024Quote: ... mouse monoclonal antibody (Sigma). FLAG ...
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bioRxiv - Synthetic Biology 2024Quote: ... mouse monoclonal antibody (Sigma). Myc ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse anti-Fibrilarin (Sigma), anti-H3ac ...
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bioRxiv - Physiology 2024Quote: ... Rabbit α-mouse Calmodulin (5μg mL-1, CellSignaling), rabbit α-mouse β-actin (1μg mL-1, CellSignaling) and mouse α-mouse GAPDH (1μg mL-1, Merck/Millipore), goat-α-mouse IRDye 680RD ...
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bioRxiv - Microbiology 2022Quote: ... kinase assays were performed by incubating the immunoprecipitated proteins and beads in 30 μl kinase buffer supplemented with 50 μM ATP and 3 μg histone H1 (EMD Millipore, 14-155) for 30 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... pneumoniae TIGR4 and TIGR4 ΔlytB strains were previously reported.(14) S.pneumoniae strains were grown in Mueller Hinton (MH) broth (Sigma Aldrich, St. Louis, MO) supplemented with 5 % (v/v ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Developmental Biology 2020Quote: ... For each ml of food 2.5 µl Pioglitazone (14 µM final concentration, 2 µg/µl stock solution, diluted in DMSO; Sigma-Aldrich, St. Louis, USA) were added ...
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bioRxiv - Microbiology 2019Quote: ... Briefly, overnight bacterial cultures in Mueller-Hinton broth (Oxoid, Basingstoke, UK) were mixed with 1% Zwittergent 3-14 detergent (Millipore, Billerica, MA, USA) in 100 mM citric acid (pH 2.0 ...
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bioRxiv - Microbiology 2021Quote: ... Peaks were identified using the mass spectra library NIST version 14 and verified and quantified using reference standards for FAME (Supelco® 37 Component FAME Mix, Sigma Aldrich, USA) and saturated alkanes (C7-C40 Saturated Alkanes Standard ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured for 14 days at 37°C in monoculture on uncoated 10-cm dishes using RPMI-1640 medium (Sigma-Aldrich, R-8758) supplemented with 10% fetal calf serum (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... Samples were hydrated by adding two-fold excess D2O (8 mg of HA dissolved in 16 μL of D2O or 14 mg of HA dissolved in 28 μL D2O) (99%, Sigma-Aldrich, Darmstadt, Germany), and kept at room temperature for 24 hours prior to solid-state NMR measurements ...
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bioRxiv - Biophysics 2023Quote: ... Stretching is performed using 2x2 cm flexible polydimethylsiloxane (PDMS) membranes (Sylgard184; crosslinker-to-base ratios of 1:14; cured for 24 h at 65°C, Sigma-Aldrich, St. Louis) as described in (45) ...
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bioRxiv - Microbiology 2023Quote: ... Hybridoma selection was carried out for 14 days by adding HAT (100 mM hypoxanthine, 0.4 mM aminopterin, and 16 mM thymidine; Sigma, St. Louis, MO, USA) to RPMI-1640 medium 24 hours after cell fusion ...
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bioRxiv - Genomics 2023Quote: ... 10 mL ATCC vitamin supplement and 1 mL vitamin K-3 solution (0.14 g vitamin K-3 in 100 mL 95% ethanol)24 reduced by the addition of 50% Oxyrase (SAE0013; Sigma, St. Louis, MO) were added in an anaerobic chamber ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... we used mouse anti-FLAG antibody (MBL) and normal mouse IgG (Sigma Aldrich) as an additional negative control ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal HA-Tag and Mouse Monoclonal Flag antibodies purchased from Sigma (USA); Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Microbiology 2021Quote: ... mouse anti-BrdU/nuclease (1:100) (Amesham) or mouse anti α-tubulin (Sigma)/1% BSA/PBS (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Mouse antibody isotypes were determined using the mouse monoclonal antibody isotyping kit (Sigma).
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bioRxiv - Neuroscience 2022Quote: ... Mouse anti-Flag and non-specific mouse IgG (4mg/ml; Sigma F7425; RRIB:AB_439687) were used for pull-down ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse anti-TRA1-60 and Mouse anti-TRA1-81 1/200 (both Millipore), Mouse anti-PAX6 ...
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bioRxiv - Immunology 2021Quote: ... mouse IgG1 anti Golgin 97 (CST) or mouse anti IgG1 PDIA3 (Sigma-Aldrich) and mouse IgG2a anti-dsRNA J2 (Scicons ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-mouse α-smooth muscle actin (αSMA) conjugated to Cy3 fluorophore (Sigma), and mouse anti-rat neural-glial antigen 2 (NG2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mouse anti-myc antibody (1 μg) or mouse IgG (1 μg) (EMD Millipore) were incubated with protein A/G Magna beads (20 μl ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA Mouse monoclonal antibody (Clone HA-7, mouse IgG1 Isotype; Sigma-Aldrich). The membrane was washed thrice in PBST and incubated with HRP-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse Vinculin (V9131) and mouse Flag (F3165) antibody were purchased from Sigma-Aldrich. Antibodies against METTL14 (26158-1-AP) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-FLAG (M2) and mouse anti-α-tubulin (clone DM1A) (Sigma-Aldrich); mouse anti-clathrin heavy chain (BD Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: HEK cells used for electrophysiological study were adhered to the poly-L-lysine-coated (MW 30,000-70,000, Sigma-Aldrich; 0.1 mg/mL for 2 hr at 37 ºC) coverslips (CS-5R ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for immunostaining include rabbit anti-human/mouse PKD-1 (4502371) and mouse anti-mouse α-smooth muscle actin(A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A hatchling was crushed in 300 μL 0.2% saline solution (Sigma-Aldrich, Germany, Cas number: 7732-18-5, Sigma-Aldrich, Germany 7647-14-5), vortexed and 50 μL of the mixture was plated onto two different Potato Dextrose Agar plates (PDA ...
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bioRxiv - Cancer Biology 2022Quote: ... 300 ug of protein in 600 uL of lysis buffer was used for the GTP pulldown with 20 ul of PAK beads (Sigma, Cat. no. 14-325) incubated for one hour ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were loaded on an 8% Tris/glycine gel and subjected to Western blot analysis using different primary antibodies (anti-c-myc (A-14, sc-769, Santa Cruz, Nunningen, Switzerland and HA antibody, rat, 3F10, Sigma-Aldrich, Munich, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...