Labshake search
Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Canine Adenovirus 1 and 2 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... total soluble protein was extracted from 50 mg of ground frozen shoot material in 250 µl 2× Laemmli buffer supplemented with 1% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich, Steinheim, Germany). Proteins were denatured for 10 min at 90°C and separated using 10% (w/v ...
-
bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
-
bioRxiv - Bioengineering 2022Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300 ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were infected at low MOI 1-2 in EBM-2 media with supplements in the presence of 8 μg/mL polybrene (Sigma-Aldrich, St.Louis, USA) and spinoculation at 450g for 30 min ...
-
bioRxiv - Biophysics 2022Quote: ... the pancreas was transferred to a foil-covered glass vial containing a 1:1 solution of MeOH (100%) and BABB (1:2 (v/v) benzyl alcohol (402834-500ml, Sigma-Aldrich, Gillingham, UK) to benzyl benzoate (B6630-1L ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then incubated in sucrose buffer (0.1 M Tris-HCl pH = 7.5, 2 mM MgCl2, 1 M sucrose, 1 % Protease Inhibitor Cocktail (Sigma Aldrich), 200 μg.mL−1 RNase A and 10 μg.mL−1 DNase (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
-
bioRxiv - Biochemistry 2022Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
-
bioRxiv - Neuroscience 2021Quote: ... and 2 % of FBS for 1 h at room temperature (RT) and incubated with PSD95 (1:1.000, MAB1598; Millipore), PrP (1:500 ...
-
bioRxiv - Genomics 2022Quote: ... G418 foci were washed with 1× PBS and fixed using 2% Formaldehyde/0.2% Glutaraldehyde in 1× PBS (Sigma-Aldrich) fixing solution at room temperature for 30min ...
-
bioRxiv - Molecular Biology 2019Quote: ... at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich) at a 1:1500 dilution ...
-
bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
-
bioRxiv - Biochemistry 2021Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: Percoll gradients (1-2 per sample depending on harvest density) were formed by vortexing 1 ml Percoll (Sigma P1644) with 110 µl 10x PBS in 2 ml tubes and centrifuging 15 min at 15,000 g ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1% Phosphatase inhibitors cocktail 2 Sigma-Aldrich Cat:P5726 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lipids were extracted using a 1:1:2 v/v ratio of methanol (Biosolve) containing 0.01% w/v butylated hydroxytoluene (Sigma), water (Biosolve ...
-
bioRxiv - Biochemistry 2022Quote: ... A 1:2 serial dilution (concentration range from 1 mM to 0 mM) of Calcium chloride (#C5080, Sigma-Aldrich), Magnesium chloride (#M2393 ...
-
bioRxiv - Biophysics 2022Quote: ... Concentrated Cav1.2(ΔC)/Cavβ3/Cavα2δ-1 sample was immediately incubated with gabapentin (final concentration 2 mg ml-1, 11.7 mM) (Sigma-Aldrich) on ice for four hours prior to cryo-EM sample preparation ...
-
bioRxiv - Developmental Biology 2023Quote: Embryos used later than 34 hours post-fertilization were kept transparent by soaking them in embryo medium (Danieau’s Solution) with 1% 1-phenyl-2-thiourea (PTU) (Sigma) to inhibit pigment formation (M. ...
-
bioRxiv - Microbiology 2023Quote: ... D-alginate was produced by enzymatically degrading 2% alginate with 1 unit ml-1 of alginate lyase (Sigma Aldrich) at 37 °C for 24 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... Medium samples from day 2 and day 4 had to be diluted 1:1 in PBS (P4417, Sigma-Aldrich) because of the higher levels of cell death in the first days after harvesting caused by the isolation procedure ...
-
bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
-
bioRxiv - Neuroscience 2024Quote: ... and incubated in the primary antibody (anti-Kv3.2 antibody, 1:200, Cat.no. APC-011, alomone lab, Israel; anti-GAD67 antibody, 1:200, Cat.no. MAB5406, Sigma, USA) at 4°C overnight ...
-
bioRxiv - Bioengineering 2020Quote: ... 2-hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 410896, Sigma Aldrich), VEGF specific control aptamer (47-nt ...
-
Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...
-
bioRxiv - Neuroscience 2021Quote: ... and then supplemented with 2% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma Aldrich 410896) as the UV initiator ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with primary antibodies overnight at 4C (DARPP-32 1:200 Abcam ab40801; FoxP1 1:500 Abcam ab16645; GAD65/67 1:500 Millipore AB1511; MAP-2 1:1000 Millipore MAB3418) and then washed three times with PBS before an overnight 4C incubation in secondary antibody (Alexa-fluor rabbit 555 IgG ...
-
bioRxiv - Microbiology 2022Quote: ... EIF4E2 shRNA#2 (sh4EHP#2) (Sigma, TRCN0000280916) and EIF4E2 shRNA#3 (sh4EHP#3 ...
-
bioRxiv - Microbiology 2022Quote: ... GIGYF2 shRNA#2 (shGIGYF2#2) (Sigma, TRCN0000138937) and GIGYF2 shRNA#3 (shGIGYF2#3 ...
-
bioRxiv - Cell Biology 2021Quote: ... 2.5% 2-Mercaptoethanol (Sigma, 60-24-2), and sonicated ...
-
bioRxiv - Neuroscience 2019Quote: ... 2-DG (2 g/kg; Sigma, Ireland), and insulin (11.5 IU/kg (400 μg/kg) ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.1 mM 2-mercaptoethanol (2-ME; Sigma), 100 U/ml penicillin and 100 µg/ml streptomycin sulfate (Nacalai Tesque ...
-
bioRxiv - Cancer Biology 2020Quote: ... with 2% v/v 2-mercaptoethanol (Sigma). The samples are immediately frozen on dry ice and subsequently stored at −80° C until library preparation and sequencing ...
-
bioRxiv - Molecular Biology 2021Quote: 2-deoxyglucose (2-DG, Sigma, D6134-250MG) was dissolved in 1x DPBS ...
-
bioRxiv - Cell Biology 2021Quote: ... 2-5mM of 2-deoxy-glucose (SIGMA) was added to the normal medium ...
-
bioRxiv - Cancer Biology 2022Quote: MI-2-2 was purchased from Millipore; MI-503 ...
-
bioRxiv - Plant Biology 2022Quote: 2-mercaptoethanol (2-ME, Sigma-Aldrich, M6250)
-
bioRxiv - Plant Biology 2023Quote: ... 2% Phosphatase inhibitor cocktail 2 (Sigma-Aldrich) and 2% Phosphatase inhibitor cocktail 3 (Sigma-Aldrich)] ...
-
bioRxiv - Neuroscience 2023Quote: ... and 2-Butanone (2-Bt; Sigma Aldrich).
-
bioRxiv - Cell Biology 2023Quote: 2-deoxyglucose (2-DG, Sigma, D6134-250MG) was dissolved in 1x DPBS ...
-
bioRxiv - Biophysics 2023Quote: ... 2-Aminoethoxydiphenyl borate (2-APB, Sigma-Aldrich) was dissolved in DMSO to produce a 1 M stock ...
-
bioRxiv - Neuroscience 2023Quote: ... 4uL 2-Mercaptoethanol (Sigma #60-24-2), supplemented with 100µg/mL Normocin (InvivoGen #ant-nr-2) ...
-
bioRxiv - Bioengineering 2024Quote: ... 2-hydroxy-2-methylpropiophenone (Sigma-Aldrich, Netherlands) was added to the hydrogel solution at a volume ratio of 1:100 to achieve efficient crosslinking ...
-
bioRxiv - Neuroscience 2023Quote: ... 2-Aminoethyldiphenylborinate (2-APB, 100 μM; Sigma), Larixyl acetate (10 μM ...
-
bioRxiv - Neuroscience 2021Quote: ... tissues underwent antigen retrieval by incubating for 15 minutes in hot 10mM sodium citrate buffer pH=6.0 (Sigma cat. no. S-4641). Sections were rinsed in PBS and incubated in 3% H2O2/PBS for 15 minutes ...
-
bioRxiv - Immunology 2019Quote: ... sections from the initial portions of sockets were incubated with primary antibody for Proliferating Cell Nuclear Antigen (PCNA) (#MAB424, Merck Millipore, Darmstadt, Germany) diluted to 1:150 in blocking solution and incubated at 4°C overnight ...
-
bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval was performed using 1X Sodium Citrate Buffer diluted from a 10X stock (10X Stock: 100mM trisodium citrate (Sigma, Cat#S1804), 0.5% Tween 20 (Thermo Fisher ...
-
bioRxiv - Immunology 2020Quote: ... Detection of GFP and Iba1 required high-temperature antigen unmasking in 0.01 M citrate buffer (pH 6.0) (Sigma-Aldrich, San Diego, CA). Sections were treated with biotin and streptavidin blocking solutions (Vector Laboratories ...
-
bioRxiv - Neuroscience 2019Quote: ... pre-incubation of the neurotransmitter antibodies used in this study with their corresponding antigen (100 μM - 400 μM; GABA (Sigma-Aldrich, A2129), glutamate (Sigma-Aldrich ...