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Citations for Millipore Sigma :
1151 - 1200 of 3699 citations for CD44 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... or 2.5 µg of human vitronectin (Sigma-Aldrich), or 5 µg of laminin (derived from mouse Engelbreth-Holm-Swarm sarcoma ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5% human platelet lysate (Sigma #SCM152) and 50 μg/ml Primocin (InvivoGen #ant-pm-2) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5% human platelet lysate (Sigma #SCM152), w/o antibiotics and exchanged to the plated cell medium ...
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bioRxiv - Immunology 2023Quote: ... and human IgE (1 µg/ml, Sigma, #401152) in regular culture medium according to (39 ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse Anti-Human TH (1:200, Millipore, MAB318). After washing with PBS cells were incubated with respective secondary Goat Anti-Mouse Alexa Fluor 546 (1:500 ...
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bioRxiv - Biochemistry 2024Quote: Human serum albumin (Sigma-Aldrich, Lot No.: SLBZ2785) was reconstituted in 1 ml of PBS to achieve a final concentration of 120 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... 40% 1mg/mL collagen from human placenta (Sigma) and 10% fibronectin from bovine plasma (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... AP-conjugated anti-human IgG (A3187, Sigma-Aldrich) was used as a secondary at 1:2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-Human Nuclei (Millipore, MAB1281, 1:200), mouse anti-STEM121 (Takara ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% AB human serum (Sigma-Aldrich), and plated onto non-treated tissue culture plates (Costar) ...
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bioRxiv - Cancer Biology 2023Quote: Human recombinant IL-6 (Sigma-Aldrich, cat. I1395) was prepared as a 50 mg/mL stock solution in PBS/1%BSA ...
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bioRxiv - Microbiology 2023Quote: ... the secondary antibody anti-human IgG-HRP (Sigma) was added at 1:1000 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... 0.01 mg/ml human recombinant insulin (Sigma, I9278), and 1% 100 mM sodium pyruvate solution ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 2 % human serum (HS, Sigma-Aldrich) at a concentration of 0.5×106 cells/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... pLKO.1 shp62 (human) was purchased from Sigma. pLKO.1 shp62 (mouse ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Heat-inactivated pooled human sera (Sigma-Aldrich, S1764) and rhesus sera were used as negative controls ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg/mL human insulin (Sigma Cat. #91077C), 0.1 nM cholera toxin (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg/ml human insulin solution (Sigma, #I9278), and 1X L-Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: Human Methemoglobin (Ferrohemoglobin) was purchased from Sigma-Aldrich, Missouri ...
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bioRxiv - Immunology 2024Quote: ... Complement C6 depleted human serum (C1288, Sigma-Aldrich), diluted to final concentration of 2% with dilution buffer (CH50 assay ...
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bioRxiv - Immunology 2024Quote: ... Non-heat inactivated normal human serum (Sigma-Aldrich) was diluted 1:18 (100% ...
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bioRxiv - Biophysics 2024Quote: ... and 5 IU human chorionic gonadotropin (hCG; Millipore) 48 h later ...
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bioRxiv - Immunology 2024Quote: ... 800 ml of human Serum (Sigma Aldrich # H4522) were thawed at 37°C after addition of cOmplete™ ...
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bioRxiv - Neuroscience 2024Quote: ... human citrated plasma (Sigma Cat No. P9523-1ML) was added and time to clot formation was recorded.
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 5% Human serum (Sigma H4522-100ML), 1% Pen/Strep (Thermofisher 15140-122 ...
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bioRxiv - Genomics 2024Quote: ... + 5% Human Serum (Sigma-Aldrich catalogue number H4522) + 1% Penicillin-Streptomycin (Gibco catalogue number 15140122 ...
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bioRxiv - Immunology 2024Quote: Recombinant human TNF-a (rTNF-α; Sigma #H8916) was resuspended in 1X PBS to a stock solution of 0.72ng/nl ...
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bioRxiv - Immunology 2024Quote: ... and endotoxin-free recombinant human Albumin (Sigma A9731) at a final concentration of 5 mg/mL ...
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bioRxiv - Immunology 2024Quote: ... and 5% human heat-inactivated AB serum (Sigma). The antibodies used for stimulation were anti-human CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2024Quote: ... an added positive human IgG Kappa (Sigma-Aldrich) loaded control lane was included (lane P) ...
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bioRxiv - Biophysics 2020Quote: Codon-optimized and his-tagged (C-terminus) genes for HG-series Kemp eliminases (Supplementary Table 1) cloned into the pET-11a vector (Novagen) via NdeI and BamHI were obtained from Genscript ...
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bioRxiv - Cancer Biology 2021Quote: Anti-Flag (Cat. number: F7425) and anti-His (Cat. number: SAB1306085) antibodies were from Sigma-Aldrich (St. Louis, MS, USA). Anti-phospho-γ-H2AX (Ser139 ...
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bioRxiv - Molecular Biology 2020Quote: Aliquots of 3 μg of GST-LC3B (Viva Bioscience) were incubated alone or with 250 ng of His-STK4 (Millipore) in in vitro kinase reaction buffer (10 mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... Y187 strains were grown on SD/-Trp-Leu medium and transferred to SD/-Trp-Leu-His medium with 10 mM 3-aminotriazole (3-AT, Sigma) in gradient dilutions (1:10 ...
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
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bioRxiv - Biophysics 2021Quote: The bacteriophage T7 gp16 gene was cloned into the pET-16b vector (ampr and 10x-His tag) from Novagen (Germany), inserted between NdeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Biochemistry 2020Quote: ... Trx-His(6)-hSLAP2 or Trx-His(6)-Δlinker-hSLAP2 were cloned in frame into multiple cloning site (MCS) 1 of pETDuet-1 (Novagen), with CBL (47-357 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Plant Biology 2021Quote: ... The cell lysate was centrifuged for 30 minutes at 5,000 x g and the supernatant was recovered and mixed with HIS-Select Nickel Affinity Gel (Sigma Aldrich) for protein purification (manufacturer’s instructions were followed) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence was excised with BamHI/EcoRI and ligated downstream of the His tag into the multiple cloning site MCS1 of pETDuet-1 (ampicillin resistance; Novagen), yielding Sll0171-pETDuet-1 ...
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bioRxiv - Biochemistry 2022Quote: ... the protein sequence fused to an N-terminal GB1 solubility tag and a 6x TEV-cleavable His-tag was cloned into pET24b (Novagen). Plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Microbiology 2020Quote: Female BALB/c mice have been injected intraperitoneally with 700 μg recombinant His-tagged TGT protein that was emulsified in complete Freund’s adjuvant (Sigma-Aldrich). Two weeks later ...