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Citations for Millipore Sigma :
1151 - 1200 of 6427 citations for 6 Pteridinol 7 8 dihydro 7 methoxy 7CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Rosa26-eYFP (Myh11eYFP) mice aged 6-8 weeks by 10 intraperitoneal injections of 1 mg tamoxifen (Sigma) dissolved in corn oil over 2 weeks ...
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bioRxiv - Molecular Biology 2020Quote: ... and 20 μl of 1 g/ml methoxy PEG thiol (average Mn ~800; Sigma-Aldrich, cat #729108). Methoxy PEG thiol was used to mask unbound maleimide groups and to prevent aggregation of spheres while demulsifying ...
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bioRxiv - Neuroscience 2019Quote: ... mice were transferred to a new cage without food at 7:30 am and injected intraperitoneally with vehicle or CNO (Sigma-Aldrich #C08325) at 8:30 am ...
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bioRxiv - Genomics 2021Quote: ... A1413301)-coated 12-well plates at a density in the range of 3-7×105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). Alternatively ...
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bioRxiv - Neuroscience 2020Quote: ... to 30 µL CSF (pooled from 1 – 3 animals) and placing in 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich). Serum samples (samples pooled correspondingly to the CSF samples ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared for NMR by combining 16 µL standard with 50 µL serum and placing in a 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
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bioRxiv - Immunology 2021Quote: ... subsequently lysed and postnuclear supernatants were incubated o/n with monoclonal mouse anti-HA-antibody coupled to agarose beads (clone HA-7, Sigma-Aldich, USA) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... used for prochloraz quantification was obtained from Rathburn (Walkerburn, Scotland), acetic acid (CAS; 64-19-7, LC-MS grade) from Sigma-Aldrich (Germany), 25:24:1 phenol:chloroform:isoamyl alcohol from Carl Roth GmbH & Co ...
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bioRxiv - Cell Biology 2020Quote: ... using N2B27 2i + Lif medium at 37°C and 7% CO2 in culture flasks coated with 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) diluted in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... for 2 hours and incubated overnight at 4°C with the primary antibodies (1/200 anti-dystrophin antibody Abcam15277, 1/50 anti-utrophin antibody Mancho 7 or 1/500 anti a-actinin antibody, Sigma-Aldrich A7732). After washing steps with PBS-Tween 0.1% ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... during 7 days, in a medium composed of equal volumes of modified C2C12 differentiation medium (DMEM, 2% horse serum (Sigma-Aldrich, #H1270),1% antibiotic-antimycotic (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Synthetic Biology 2022Quote: ... the reaction mixture consisted of 125 µl of 55 mM phosphate buffer adjusted to pH 7 and 10 µl of the substrate 1 mM ρ-nitrophenyl butyrate (PNPB) (Sigma N9876) homogenised in 2-propanol ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral supernatants were generated by transfecting the plasmids into 293T cells and used to infect 8988T cells that were then selected for at least 7 days with 2 μg ml−1 puromycin (Sigma-Aldrich, P8833). The RNAi consortium clone IDs for the hairpins used were ...
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bioRxiv - Plant Biology 2021Quote: ... mixed with 0.1 or 0.01% (w/v) CO solution and CNF dispersion in DW at 1% (w/v) in magenta boxes (GA-7; Sigma-Aldrich, USA). Plants were grown in a growth cabinet (BiOTRON ...
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bioRxiv - Biochemistry 2021Quote: ... a biotinylated DNA oligonucleotide complementary to the target tRNA (Supplementary Table 7) was immobilized on Novagen Streptavidin Agarose resin (Merck-Millipore, Burlington, MA) in a 0.22 μm Ultrafree-MC filter cup (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: Recombination of the Bbs5flox/flox allele was induced in juvenile CAGG-creERT2 (Jax Mice stock# 004682) positive mice at postnatal day 7 by a single intraperitoneal (IP) injection of 9 mg tamoxifen (Millipore Sigma, T5648) per 40 g body weight ...
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bioRxiv - Immunology 2020Quote: ... Human breast adenocarcinoma cell line (MCF-7) were obtained (ATCC, Manassas, VA, USA) and maintained in DMEM-F12 (Sigma-Aldrich, MO, USA) with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2021Quote: ... COS-7 (hamster, DSMZ Germany, ACC 60), and Rat-1 (rat, ATCC, CRL-2210) cells were cultured in DMEM (Sigma-Aldrich D6429) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Methyl methanesulphonate (MMS) (#129925) (CAS registry number 66-27-3) and carbendazim (#378674) (CAS no. 10605-21-7) were purchased from Sigma-Aldrich (Merck), UK.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
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bioRxiv - Cell Biology 2022Quote: ... refer to the publication from Susnik et al.32 Similar synthesis protocol was adapted for 59 nm SiO2-RhoB NP using a RhoB isothiocyanate (RBITC) dye (CAS 36877-69-7, Sigma-Aldrich, USA). For 119 nm SiO2-Cy5 NP synthesis ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were generated by culturing marrow cells in poly-L-lysine coated culture plate for 7 days with 50 ng/ml M-CSF in RPMI 1640 (Sigma-Aldrich, R8758) supplemented with 20% FBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were extracted on ice from 5 to 7 technical replicates of each soil sample by adding 0.5 mL phenol:chloroform:isoamyl alcohol (25:24:1) (Sigma-Aldrich, St. Louis, MO, USA) to 0.5 g of soil in a 2 ml Lysing Matrix E tube (MP Biomedicals ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated on a rotator at room temperature for 1 hour with 7 µg/mL final concentration Immunoglobulin G1 (Sigma-Aldrich M5284) and then affinity purified with Protein G Magnetic Beads (Pierce) ...
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µL of 10 mM of D-Val-Leu-Lys 7-amido-4-methylcoumarin (D-VLK-AMC) (in 10 % DMSO/H2O) (Sigma-Aldrich, #V3138) as plasmin substrate (25 ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: Spirodela was grown on half-strength Schenk and Hildebrandt (½ SH, Duchefa Biochemie, #S0225) liquid medium in MagentaTM GA-7 containers (Sigma Aldrich, #V8505), culture dishes (Greiner Bio-One #664160) ...
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bioRxiv - Neuroscience 2024Quote: ... a group of animals was orchidectomized using ketamine (65 mg/kg) and xylazine (7 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) injected intraperitoneally as anesthetics ...
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bioRxiv - Molecular Biology 2024Quote: ... The following day membranes were washed 3-times with TBST for 7 min and incubated with corresponding solutions of anti-mouse (A9044, Sigma; 1:15,000) or anti-rabbit (A9169 ...
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bioRxiv - Neuroscience 2024Quote: ... embryos from all genotypes (N = 7 per genotype) were collected at 40 hpf and fixed with 4% paraformaldehyde (PFA; Sigma, Gillingham, UK) in phosphate buffered saline (PBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Protoplasts of Exiguobacterium Strain-Molly (EM-P) were generated by growing the cells in 7%DSS-TSB with Lysozyme and Penicillin G (Sigma Aldrich, Germany). Transformation to protoplast was confirmed by a change in cell morphology (Carl Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 ml of RPMI + 2% Bovine Serum Albumin (BSA, heat shock fraction, protease free, essentially globulin free, pH 7, Sigma, Catalog #A3059) to a concentration of ∼2 x 108 CFU/ml ...
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bioRxiv - Plant Biology 2023Quote: ... 600 μl aliquots of diluted chromatin were incubated with 7 μl of the rabbit anti-trimethyl-Histone H3 (Lys27) antibody (Millipore, 07-449) in a rotator at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were fixed for 7 minutes in 2% formaldehyde/0.2% glutaraldehyde solution at room temperature and further incubated with X-Gal (Sigma, Cat. no. B4252) solution for one hour at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Neural crest intermediates were defined with 7 days of 12 ng/mL FGF2 and 10 μM of the activin–nodal inhibitor SB431542 (Sigma, cat# S4317) in CDM ...
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bioRxiv - Cell Biology 2023Quote: ... and the remaining pellet was resuspended in lysis buffer (10 mM HEPES pH 7, 1 mM EDTA, 1% NP-40, 500 mM NaCl, Benzonase [Millipore] and PIC) and sonicated at 10-20% amplitude for three times ...
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bioRxiv - Cancer Biology 2024Quote: ... Huh-7 spheroid formation was induced by seeding 3,000 cells in DMEM medium supplemented with 0.24% methylcellulose (Sigma-Aldrich, Zwijndrecht, The Netherlands) in a U-bottom 96-well plate (Greiner bio-one ...
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bioRxiv - Biochemistry 2024Quote: The activity of the enzyme was estimated by measuring the rate of hydrolysis of the fluorescent substrate L-leucine-7-amido-4-methyl coumarin (Leu-AMC, Sigma–Aldrich, L2145) followed at 460 nm (excitation at 380 nm ...
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bioRxiv - Cell Biology 2024Quote: ... buffered at pH 7 and loaded on a size-exclusion chromatographic (SEC) column (Superdex 200 10/300 GL; Sigma #17-5175-01). The column was equilibrated with NaP buffer at pH 7 and eluted with the same buffer at a rate of 18 mL/h ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS for 30□min at room temperature and subsequently fixed in 4% paraformaldehyde (PFA, Sigma-Aldrich, St. Louis, MO, USA; 15,812-7) in PBS at 4□°C for 1 hour ...
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bioRxiv - Biophysics 2019Quote: ... was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC, Sigma-Aldrich, Sintra, Portugal) to 7-amino-4-methylcoumarin (AMC ...
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bioRxiv - Molecular Biology 2019Quote: ... Plates were fixed after up to 7 days of IAA treatment upon initial plating and stained for alkaline phosphatase (AP) activity (Sigma, cat. 86R-1KT) according to the manufacturer’s protocol.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The following primary antibodies were applied for 24 h (or 48 h for anti-cFos antibodies) at 4°C: mouse anti-APC/CC1 Ab-7 (Millipore, OP80, 1:100), chicken anti-mCherry (Abcam ...
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bioRxiv - Bioengineering 2021Quote: ... the hydrogel was aliquoted into 125 µL aliquots and each aliquot was mixed with 25 μL of thrombin (7 U/mL, Sigma Aldrich, Cat# T6884). Immediately after mixing ...