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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for 3 2 Isopropylphenyl 1 propene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... CCCP 2 µM and Antimycin A 1 µM + 1 µM Rotenone (Sigma). Measurements were taken over 2-min intervals ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal against alpha-tubulin (1:1000; b5-1-2, Sigma, T6074). Actin was stained using 100 nM of phalloidin-iFluor488 for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse tubulin (clone B-5-1-2, Sigma, T5168-.2ML; 1/5000), and p21 Waf1/Cip1 (clone 12D1 ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-Tubulin (1:3,000; Sigma clone B-5-1-2, T5168), and mouse anti-b-Actin (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... is added 1:1 together with 2 µg/mL doxycycline (Sigma-Aldrich) to induce TetO gene expression ...
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bioRxiv - Microbiology 2024Quote: ... + 2% HI FBS + 1% Anti-anti + 1% (w/v) carboxymethylcellulose (Sigma-Aldrich)) was added to the plate (180 µL/well) ...
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bioRxiv - Microbiology 2024Quote: ... + 2% HI FBS + 1% Anti-anti + 1% (w/v) carboxymethylcellulose (Sigma-Aldrich)) was added to cells ...
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bioRxiv - Plant Biology 2024Quote: ... anti-microtubule antibody (1:2,000; clone B-5-1-2; Sigma- Aldrich), and ECL anti-mouse IgG horseradish peroxidase-linked whole antibody (1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: ... 2 DIV differentiated cells were treated with 1 µM CDK1/2 inhibitor III (Millipore, 217714) in control medium supplemented with 2% FBS for 24 h ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in pre-chilled 1mL of 2:2:1 acetonitrile (34851, Millipore Sigma) + methanol (439193 ...
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bioRxiv - Bioengineering 2021Quote: ... The CGCaP composites were then crosslinked via carbodiimide chemistry in a PBS solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Sigma-Aldrich) and N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Bioengineering 2020Quote: ... The film crosslinking level was controlled by incubating the coated scaffolds in 30 or 70 mg/mL 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma, France). The film crosslinking level has an effect on the film stiffness as shown in previous studies [23] ...
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bioRxiv - Bioengineering 2020Quote: Crosslinking of scaffolds were prepared by rehydrating scaffolds in PBS overnight with 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDAC, Sigma-Aldrich) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Microbiology 2022Quote: ... and 20 uL of 120 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (Pierce; dissolved in 47% ACN, 47% water and 6% HPLC-grade pyridine (Sigma Aldrich)) were added ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were then chemically cross-linked to the membrane during 90 min at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma Aldrich). Membranes were prehybridized for 30 min in PerfectHyb™ plus (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... and Grace Bio-Labs reusable CultureWellTM gaskets (3 mm x 1 mm, well capacity 3-10 ul, Sigma-Aldrich, GBL103250-10EA) were cleaned 3 times sequentially in 100% isopropanol and Milli-Q H2O ...
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bioRxiv - Immunology 2023Quote: ... The immobilized liposomes were completely removed with an injection of 40 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma-Aldrich) (20 μl ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (Supplementary Table 1) in 3% dried milk (Marvel) or 3% BSA (Sigma Aldrich) at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... where 10 seedlings each were incubated 2.5-3 h in the dark at RT with agitation in 3 ml of liquid medium (same as above) supplemented with either 3 µl of 1 mM Concanamycin A (Sigma, C9705) dissolved in DMSO or with 3 µl DMSO alone (control) ...
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bioRxiv - Genomics 2023Quote: ... then quickly was cut to small pieces (1-3 mm) in petri dish with 3 -5 ml RMPI medium containing 0.05mg/ml TM Liberase (Sigma Aldrich, 5401119001) and 0.02 mg/ml DNAaseI (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... in 0,5× TBE buffer for 1h at 350 mA of current and EDC-cross-linked for 45 min at 60°C with a solution containing 33 mg/ml of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Sigma Aldrich), 11 ng/ul of 1-methylimidazol and 0.46% of HCl ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were then prepared as 1% w/v suspension in 100 mg/mL of filter sterilized 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide-HCl (EDAC; E2247, Sigma-Aldrich) in activation buffer ...
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bioRxiv - Genetics 2021Quote: ... 3 mM MgCl2 (Sigma, 7786-30-3), 0.1% Tween-20 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and EIF4E2 shRNA#3 (sh4EHP#3) (Sigma,). GIGYF2 shRNA#1 (shGIGYF2#1 ...
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bioRxiv - Cancer Biology 2022Quote: ... covered with 3-aminopropyltrimethoxysilane (3-APTS, Sigma) for 3 min for activation ...
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bioRxiv - Neuroscience 2021Quote: ... oxygenated DAB (3-3’-diaminobenzidine, Sigma-Aldrich) was dissolved in 0.1 N HCl at a concentration of 5.4 mg/ml and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 3-mercaptobenzoic acid (3-MBA, Sigma-Aldrich)-capped gold nanoclusters (3-MBA-Au nanoclusters ...
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bioRxiv - Biochemistry 2024Quote: ... 3% SDS (Sigma-Aldrich, 151-21-3), 25 mM β-glycerophosphate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mM 3-methyladenine (3-MA, Sigma) or water (vehicle)46 ...
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bioRxiv - Cancer Biology 2024Quote: ... ultracel-3 (>3 kDa cutoff) concentrators (Millipore). 30 µl of concentrate was loaded for western blot analysis.
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bioRxiv - Neuroscience 2022Quote: ... For the blockade of cholinergic receptors in CA1 200nl of atropine (2 mM) and mecamylamine (2 mM) (1:1) (Sigma Aldrich) were microinjected at 1.2mm DV (IM-9B microinjector ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with anti-Sulf-1/2 primary antibody (anti-Sulf-1: rabbit polyclonal, Sigma, SAB1410410, anti-Sulf-2: rabbit monoclonal clone 2B4, Sigma, MABC584) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of 2 mg/mL IG[pS]TENLK was mixed 1:1 with 250 mM Ba(OH)2 (Sigma-Aldrich) in a total volume of 200 μL and incubated at 37°C for 90 min ...
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bioRxiv - Bioengineering 2024Quote: The alkaline comet assay was performed by mixing 1.5 × 105 cells 1:1 with 2 % low melting point agarose (2-Hydroxyethylagarose, Type VII, Sigma-Aldrich, A4018) at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... supplemented with 10 g L−1 sucrose and 3 g L−1 phytagel (Sigma, Steinheim, Germany) or 8 g L−1 agar (Winkler ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg ml-1 of 3–5 kDa fluorescein isothio-cyanate (FITC)-dextran (Sigma-Aldrich, Germany) in phenol-red free DMEM/F12 medium (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The cleaned dishes were incubated with 1 mL of 1% (3-aminopropyl) triethoxysilane (APTES; Sigma Aldrich) in ethanol for 1hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were infected with pLKO.1 control or pLKO.1-shZSCAN4 (5’-GAATGCAACAACTCTTGTAATCTCGAGATTACAAGAGTTGTTGCATTCT-3’, Millipore Sigma) and further selected with Puromycin (1ug/ml ...
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bioRxiv - Biophysics 2024Quote: ... and sn-glycero-3-phosphocholine 1:1 cadmium chloride adduct were all purchased from Sigma-Aldrich, with the exception of acarbose ...
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bioRxiv - Systems Biology 2022Quote: ... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated on ice for 3 min and 2 mL wash buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1 % Tween-20, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Cancer Biology 2021Quote: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (L’Isle-d’Abeau-Chesnes, France). Antibodies against phospho-IκBα Ser32 (#2859) ...
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bioRxiv - Neuroscience 2021Quote: Prior to training rats were randomly assigned to receive either sham surgery or intracranial bilateral lesions to the region of interest under 2-3% isoflurane anaesthesia with local application of bupivacaine (2mg/kg s.c. at 0.8ml/kg; Sigma) at the incision site ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %). The treatment was counterbalanced between trials ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %).
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bioRxiv - Cancer Biology 2021Quote: ... Metabolic activity was measured by adding 10 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 10 mg/ml, Sigma). After four hours of incubation ...