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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and 1% each of Phosphatase Inhibitor Cocktails 2 and 3 (Millipore-Sigma). Collected lysates were sonicated on ice with a probe sonifier (Branson ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% each of Phosphatase Inhibitor Cocktails 2 and 3 (Millipore-Sigma). Collected lysates were sonicated on ice with a probe sonifier (Branson ...
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bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitors (1:100 Phosphatase inhibitor cocktail 2 (Sigma Aldrich, #P5726), 1:100 Phosphatase inhibitor cocktail 3 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and counterstained with 4,6-diamidino-2-phenylindole (DAPI, 1:1000; Sigma-Aldrich). Bacterial distance to the epithelium was measured at five separate random points within five distinct regions of the proximal colon using the Volocity software (Quorum technologies ...
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bioRxiv - Cell Biology 2024Quote: ... and supplemented with 200 mM 1-phenyl-2-thiourea (Sigma-Aldrich, P7629) to inhibit melanogenesis ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Plant Biology 2021Quote: ... Five days after germination the plant were incubated in liquid in ½ MS with 1% sucrose media with or without 50 µM 2-bromopalmitate (2-BrP; Sigma-Aldrich), and with or without 100 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... were then transferred to 2 mL microtubes containing 1 mL of lysis buffer [2% (m/v) cetyltrimethyl ammonium bromide (Sigma Aldrich), 1.4 M NaCl ...
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bioRxiv - Immunology 2020Quote: PBMC or Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-1/2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with PBST and then incubated for 2 h at room temperature in PBS with 2 μg mL−1 DAPI (D9542, Sigma-Aldrich). Cells were imaged using an LSM980 confocal microscope (Zeiss) ...
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bioRxiv - Plant Biology 2022Quote: ... 0.85 % saline (2 mins), Phosphate Buffered Saline (PBS) (2 mins), 0.125 mg ml-1 Pronase (37°C, 10 mins) (Sigma-Aldrich, P2308), 0.2 % Glycine (2 mins ...
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Strains were streaked from frozen stock onto yeast extract peptone dextrose (YPD; 1% yeast extract, 2% peptone, 2% dextrose, Sigma-Aldrich) agar and left overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Microbiology 2022Quote: ... were place on the plate and 10 μL of (1, 2, 4 and 9.8 M) H2O2 and (2, 4, 6 M) methyl viologen (Sigma-Aldrich, UK) was dispensed onto each separate disk ...
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bioRxiv - Cell Biology 2023Quote: ... on ice in LCMS-grade acetonitrile/methanol/water (2:2:1 v/v) with 1nmol scyllo-inositol internal standard per sample (Sigma Aldrich). Sample debris was then removed via centrifugation and polar metabolite extracts were dried using a SpeedVac Vacuum Concentrator ...
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bioRxiv - Biophysics 2023Quote: Crude and low-dense MVs were exposed to SCL medium supplemented with CaCl2 (2 mM) and either 2 mM Na2HPO4 or 1 mM adenosine triphosphate (Sigma-Aldrich). Mineralization was carried out at 37°C and using samples with fixed 50 µg of total protein/mL concentration ...
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bioRxiv - Plant Biology 2024Quote: ... 50 mg aliquots of homogenized tissue were incubated with 1 ml CTAB buffer (2% w/v) + 10 µl 2-mercaptoethanol (Sigma M3148) at 55°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM MgOAc, 1 mM CaCl2, 200 mM sorbitol, 2 mg/mL iodoacetamide, and protease inhibitors [Pierce A32965 or Sigma 11836153001]). Resuspended pellets were dripped slowly into liquid nitrogen to form “popcorn” and were stored at −70°C until further processing ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 µg 14:0 Lyso PE and 0.4 µg (14:0)2-PS in a final volume of 90 µl of chloroform/methanol 2/1 (Sigma Aldrich, France). Lipids were extracted according to the method of Folch with 4410 µl of chloroform/methanol 2/1 for 1 h followed by 1250 µl of saline for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then centrifuged at 10,000×g for 2 minutes and incubated with 1 mL of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich 93443) for 30 minutes with gentle agitation ...
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bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Bioengineering 2020Quote: ... Serial dilutions of the samples (1:2 to 1:64) and a standard row containing 1 to 40 nmol Neu5Ac (Sigma Aldrich) were prepared in extraction buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA, 0.5 mM DTT, 1 mM NaF, 1 mM PMSF and 1% Protease Inhibitor Cocktail from Sigma, pH 7.4) for 30 min on ice ...
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bioRxiv - Neuroscience 2021Quote: ... a mouse monoclonal anti-chondroitin sulfate antibody (clone CS-56, 1:500 for Experiment 1 and 3 or 1:1000 for Experiment 2, C8035, Sigma Aldrich) specific for the glycosaminoglycan portion of the chondroitin sulfate proteoglycans that are the main components of the PNN and a polyclonal rabbit anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The fresh tissue was dissected followed by digestion for 2 hours at 37°C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich). Debris was removed and cells isolated by sequentially passing through 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were ground in an ice-cold buffer (50 mmol.L-1 TRIS, 5 mmol.L-1 EDTA, 2 mmol.L-1 DTT, protease inhibitor cocktail (Sigma, St. Louis, MO, USA)) ...
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bioRxiv - Developmental Biology 2023Quote: ... 90%, 100%), incubated in acetone/Spurr resin (1:1, 30 minutes, 1:2, 30 minutes) and in Spurr resin (Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich) and 5 ng/ml FGF2 (Peprotech) ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then incubated in a solution of 1:1 100% methanol and BABB (1:2 solution of Benzyl alcohol (BA; Sigma, 24122) and Benzyl benzoate (BB ...
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bioRxiv - Pathology 2021Quote: ... were starved 6 hours from Zeitgeber 14 (ZT14) and refeed for 8 hours with the CD and 20% glucose (Sigma-Aldrich, Saint Louis, MO, USA) in the drinking water.
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bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... 2 mM 2-Mercaptoethanol (Sigma-Aldrich; Cas: 60-24-2), and 4 mM 4-Nitrophenyl acetate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS 1X and incubated during 1h at room temperature with Alexa FluorTM 594 donkey anti-rabbit IgG (H+L) (Sigma-Aldrich, cat. nr A21207) secondary antibody 1:500 was used ...
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bioRxiv - Microbiology 2020Quote: ... and 1 mM tris(2-carboxyethyl)phosphine (TCEP)] containing 1 SIGMAFAST protease inhibitor EDTA-free cocktail tablet (Sigma). Due to the presence of two remaining cysteines in PelXC232S ...
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bioRxiv - Systems Biology 2020Quote: ... secondary antibody was added (Goat anti-rabbit HRP, 1% milk, TBST 0.2%, 2 h, RT, 1:5000; Sigma). Once washed ...
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bioRxiv - Neuroscience 2020Quote: ... SYSY), vesicular glutamate transporter 2 (VGLUT2; 1:1,000, SYSY) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:50,000, Millipore) at 4 °C for 16 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells in Accutase were added 1:2 to mTeSR™1 plus 5 μM Blebbistatin (Bleb, B0560, Sigma), pelleted at 150g for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... 10 mM MgCl2,10 mM Tris pH 7.4, 1 mM CaCl2) with increasing amounts (0, 0.5, 1, 2, 5, 7.5, 10 U) of MNase (Sigma) at 30°C for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation of secondary antibodies (1:500) for 2 h and DAPI (1:10,000, D9542, Sigma-Aldrich) in PBS for 5 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Physiology 2023Quote: ... the gonads were incubated overnight in the dark at 4 °C with secondary goat anti-rabbit IRDye 680RD-conjugated antibody (LiCor) 1:1000 in PBST/0.1% BSA and 1 ng/µl 4,6-diamidine-2-phenylindole (DAPI, Sigma). The gonads were mounted on 2% agarose pads in Fluoromount-G (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DMEM without serum contained 1 µg of L-1- tosylamide-2-phenylethyl chloromethyl ketone-treated trypsin (TPCK; Sigma) for activation of the virus infectivity ...
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bioRxiv - Neuroscience 2024Quote: LL-341070 was provided by Autobahn Therapeutics as a solution in 1% 1-methyl-2-pyrrolidone (Sigma 270458) and 1% Kolliphor HS 15 (Sigma 42966 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were routinely passaged in a 1:6 ratio every 2 days using 1× Trypsin–EDTA solution (Sigma) at 37°C and the reaction was stopped using 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... the viral medium was removed and the wells overlaid with a 1:1 solution of 2% agar (Sigma) and 2X DMEM containing 5% (v/v ...