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Citations for Millipore Sigma :
1101 - 1150 of 2451 citations for Rpb1 CTD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were activated in vitro with 2 μg of SIINFEKL peptides (Sigma) in the presence of recombinant human IL-2 (50 U/ml ...
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bioRxiv - Biochemistry 2022Quote: ... then eluted in 100 mM elution buffer + 1 mg/ml 3× Flag peptide (Sigma) at 4°C for 30 min.
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bioRxiv - Neuroscience 2022Quote: ... lyophilized peptides were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Cat# 52517, Millipore Sigma) to ensure that the starting material was in a homogenous ...
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bioRxiv - Physiology 2023Quote: ... Samples containing 4 μg of peptides were de-salted using C18 Zip-tips (Millipore) and resuspended in 20 μl of 2.5 % (v/v ...
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bioRxiv - Immunology 2022Quote: Antigen mixture for immunization was prepared by mixing OVA(323–339) peptide (Sigma-Aldrich) or NP-OVAL (Ovalbumin ...
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bioRxiv - Neuroscience 2022Quote: ... the complex was eluted using 0.15 mg/ml 3×FLAG peptide (Millipore Cat# F4799) in GDN buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting peptide mixture was desalted using C18-Zip tips (EMD Millipore, Billerica, MA), speed-vacuumed to dryness and suspended in 0.1% formic acid for injection into ...
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bioRxiv - Neuroscience 2023Quote: ... The precipitated sample were eluted with 100 μg/ml 3XFLAG peptide (Sigma-Aldrich, F4799). The eluted samples were split in half for reducing (mix with 5x SDS sample buffer at 100°C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted in two column volumes of 0.2 mg/ml 3xFLAG peptide (Sigma) and dialyzed against 50 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... peptide samples were loaded onto an Ascentis® ES-C18 Column (Sigma–Aldrich, USA) and were eluted from the column by using a 10 minute gradient from 98% solvent A (0.1 % FA in H2O ...
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bioRxiv - Cell Biology 2023Quote: ... The recovered tryptic peptides were resuspended in 0.1% trifluoroacetic acid (Sigma-Aldrich; cat. #T6508) and desalted with ZipTips C18 Pipette Tips (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted using 10 mM glutathione or 0.5 mg/mL 3XFLAG peptide (Sigma) in Lysis Buffer with 2 mM DDM.
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bioRxiv - Developmental Biology 2023Quote: ... 10 µg of peptides were further desalted with C18 ZipTips (Merck Millipore, Darmstadt, Germany) to ensure complete removal of beads.
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bioRxiv - Molecular Biology 2023Quote: ... VCF1 C-terminal peptide stocks (150 μM) were prepared in pure DMSO (Sigma Aldrich). Threefold serial dilutions of VCF1 proteins or peptides were prepared in the SPR running buffer from the primary stocks starting from highest concentration of 300 nM (VCF1 C-terminal peptides ...
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bioRxiv - Physiology 2023Quote: ... Peptides from each of these fractions were cleaned up using C18 ZipTip (Millipore, USA). Each fraction was then analysed on a quadrupole-TOF hybrid mass spectrometer (TripleTOF 6600 ...
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bioRxiv - Physiology 2023Quote: ... peptides were dissolved in 18:1:1 (by volume) of saline:Kolliphor EL (Sigma Aldrich):DMSO and administered to mice by intraperitoneal injections at a volume of 10 µl/g at the indicated doses for the indicated times ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were eluted in 10 µl 5 % DMSO in LC-MS water (Sigma Aldrich) in an ultrasonic bath for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Immunology 2023Quote: ... and protein complexes were eluted with 150 mg/ml of 3X FLAG peptide (Sigma). Samples were diluted in 2X SDS sample buffer and analyzed with SDS–PAGE according to standard procedures ...
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bioRxiv - Biochemistry 2023Quote: ... 15 µg of peptide was incubated with 50 µl of 33 mM iodoacetamide (Sigma) in degassed 5% formic acid (pH 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... the immune complexes were eluted with 150 ng/µL 3×FLAG peptide (Sigma-Aldrich) in TBS solution for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 10 µL 5% DMSO in LC-MS water (Sigma Aldrich) in an ultrasonic bath for 10 min.
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bioRxiv - Systems Biology 2024Quote: ... Samples containing 4 µg of peptides were de-salted using C18 Zip-tips (Millipore) and resuspended in 20 µl of 2.5 % (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were resuspended in MS buffer containing 2% ACN in 1% Formic Acid (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen) were amplified with PCR using Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were identified by indirect immunofluorescence using an anti-myc antibody (C3956, Sigma-Aldrich). SW13-human adrenocarcinoma vimentin-deficient cells ...
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bioRxiv - Microbiology 2024Quote: ... Samples were eluted in 45 µL of FLAG peptide (300 µg/mL, Millipore Sigma) in Wash Buffer plus 0.05% RapiGest (Waters Corp ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was eluted with 100 µL of 1.5 mg/mL FLAG peptide (Millipore) resuspended in wash buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The blocking buffer used was 1% bovine serum albumin (Sigma-Aldrich, St. Louis MO, A7906-50G) (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with rabbit anti-detyrosinated tubulin antibody (AB3201, Sigma; 1:1000 in blocking buffer) for 1h at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Larvae were then incubated in a blocking solution of 2% Normal Goat serum (Sigma #S26-LITER), 1% BSA (Fisher#BP1605-100) ...
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bioRxiv - Neuroscience 2021Quote: ... After blocking (5% skim milk in Tris-buffered saline + 0.01 % Tween®20 (Sigma-Aldrich, USA) for 1 h at RT) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a PVDF membrane and incubated in 1% Western blocking reagent (Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Blocking buffer was formulated on site by adding 10.0g bovine serum albumin (Sigma, St. Louis, MO) in 1000 mL of phosphate buffered saline (PBS ...
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bioRxiv - Pathology 2019Quote: ... at 4°C for 15 min and then subjected to blocking with 3% albumin (Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... was used to coat the surface followed by blocking with 5% pluronic F-127 (Sigma #P2443) for five minutes each step ...
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous EPSCs were isolated by blocking chloride channels with the addition of picrotoxin (75uM; Sigma-Aldrich) into the recording ACSF ...
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bioRxiv - Bioengineering 2020Quote: ... wells were blocked with blocking buffer (PBS + 0.05% Tween 20 (PBST) containing 5% skim milk (Sigma)) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Additional blocking by incubation with avidin and biotin (both 0.001% in PB, Sigma-Aldrich, Darmstadt, Germany) for 30 min and a 10 min wash with PBST in between were carried out before incubation with the blocking solution ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 30 min and blocked for 1 h with blocking solution (Merck-Millipore, Burlington, MS, USA) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS and 200 ug/ml Digitonin (D141-100MG ...
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bioRxiv - Microbiology 2021Quote: ... permeabilization and blocking was performed as described previously.83 Primary M2 anti-Flag antibody (Sigma F1804) was used at 1:250 overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were incubated overnight at 4 °C in blocking solution MABTr/10% sheep serum (Sigma-Aldrich)/2% Blocking solution (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Blocking was performed at room temperature for 2 hours using 5% skimmed milk (70166, Sigma-Aldrich) in 1X PBS containing 0.05% Tween 20 (P1379 ...
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bioRxiv - Bioengineering 2022Quote: ... primary antibodies were prepared at 1:100 in a 1:4 blocking solution (2% BSA (Sigma), 10% normal goat serum (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking was achieved by incubation with PBS with 10% donkey serum (Sigma-Aldrich; Cat. No. D9663) and 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Glutathione magnetic agarose beads were blocked overnight at 4°C with 2x blocking buffer (Sigma, B6429) then incubated with RAF-RBD-GST supernatants for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking with 10% normal donkey serum was followed by ERMN primary antibody (1:200, Sigma HPA038295) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... cochlear samples were first incubated by blocking solution containing 1% Triton X-100 (X100-500ML, Sigma) and 5% Bovine Serum Albumin (BSA ...