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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... was used to prepare unstimulated cells or FCS (Biosera or Sigma) to prepare stimulated cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Media were supplemented with 5% fetal calf serum (FCS) (Sigma-Aldrich) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with RPMI containing 10% (v/v) FCS (Sigma) and 2 mM L-Glutamine (R10) ...
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bioRxiv - Immunology 2023Quote: ... and 10% (v/v) heat-inactivated fetal calf serum (FCS) (Sigma). Cells were added to sterile 96 well U-bottom tissue culture plates pre-coated with purified NA/LE anti-CD3 (Clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich #A2429), at a 1:4000 dilution was added ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µM ABA or 1 µM Fusicoccin (FC) (all from Sigma) dissolved in absolute ethanol ...
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bioRxiv - Cell Biology 2020Quote: ... and containing 125 pg/mL recombinant IL-15 (Sigma-Aldrich) to aid uNK cell maturation ...
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bioRxiv - Microbiology 2021Quote: ... these recombinant plasmids were introduced into Tuner (DE3) cells (Novagen). For co-expression of mxiK and spa33 ...
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bioRxiv - Biochemistry 2022Quote: ... the recombinant GP was first treated with PNGase F (Sigma) to remove N-glycans ...
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bioRxiv - Genomics 2019Quote: ... and 1,000 U/ml recombinant leukemia inhibitory factor (LIF) (Millipore). For native CoBATCH experiments ...
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bioRxiv - Pathology 2021Quote: ... and 1,000 U of recombinant leukemia inhibitory factor (ESGRO; Millipore) on mitotically inactivated feeder cells as described (Hughes and Saunders ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant mTOR was purchased from Millipore (14-770) and recombinant 4EBP1 was purchased from Millipore (516678). Flag-STK11IP or 4EBP1 was incubated with or without mTOR in 50 μl reaction mixtures at 37°C for 60 mins ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant ubiquitin was purchased from Sigma Aldrich (79586-22-4).
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant CDK5/p25 (60 ng) (EMD Millipore, Burlington, MA, USA) was combined with purified ACSS2 (2ug ...
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bioRxiv - Genetics 2022Quote: ... and 10 ug/L recombinant leukemia inhibitory factor (LIF) (Millipore). Mouse embryo stem cells was cultured at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... 25 ng/ml recombinant basic fibroblastic growth factor (FGF, Millipore), and 100 U/mL of penicillin/streptomycin ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich), and 0.5 μg/ml anti-CD180 (RP/14 ...
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bioRxiv - Immunology 2020Quote: ... then stimulated with 100U/mL recombinant murine IFNβ (Sigma, I9032) or equivalent volume of water for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µl recombinant RNase inhibitor (1 U/µl, Sigma-Aldrich) was added to 390 µl internal solution to increase RNA yield ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml mouse recombinant IL-4 (Sigma-Aldrich) to allow B cell activation and class switch recombination to IgG1 ...
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bioRxiv - Bioengineering 2023Quote: ... recombinant insulin-like growth factor (R3-IGF) (Sigma Aldrich, #85580C), heparin (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... All recombinant plasmids were transformed into E.coli Rosetta (DE3) (Novagen) cells and grown in LB broth at 37°C for 3 h ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant AMPK (α1, β1, and γ1, 0.5 ng/µL, Sigma), purified truncated ACC (250 ng/µL) ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Cell Biology 2019Quote: ... Washed cells were treated with TRITC tagged Concanavalin A (20 µg ml-1) (Sigma, USA) for 1 h at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
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bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma, #A2220). For the IP ...
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bioRxiv - Physiology 2021Quote: ... followed by incubation with fluorescent-tagged carboxylate-coated 2 um size latex beads (L3030, Sigma) for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
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bioRxiv - Genetics 2022Quote: V5H6-tagged FRQ was immunoprecipitated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog #7345) from 2 mg of centrifugation-cleared lysate ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD3 and PFD5 were detected using anti-FLAG rabbit antibody (1:5,000, Sigma), FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... myc-tagged TEV protease was detected with a mouse monoclonal anti-myc antibody (Sigma M4439). The proteins were detected using SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2024Quote: ... Flag-tagged Scube2 was detected by using polyclonal α-FLAG antibodies (Sigma, St. Louis, USA). GAPDH ...
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bioRxiv - Pathology 2021Quote: HIF-1α protein expression was depleted in human U937 cell line (ATCC, USA) using shRNA targeting HIF-1α (TCRN0000010819, Sigma Aldrich, USA) using a liposome mediated delivery (Life Technologies ...
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bioRxiv - Biophysics 2024Quote: ... γ-cyclodextrin (γ-CD, CAS#7585-39-9), C-reactive protein (CRP, AG723) and human serum (H4522-100ML) were ordered from Sigma-Aldrich. 1 µM γ-CD stock suspension was prepared with MilliQ ...
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bioRxiv - Cell Biology 2021Quote: ... 4°C) and the cell pellets washed twice with 10% FCS in PBS and resuspend in Dapi solution (Sigma; 50μg/ml in 10% FCS in PBS; 100μl/well) and stored at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 10% fetal calf serum or FCS (Sigma-Aldrich, St. Louis, MO, USA) as well as 1% antibiotics (penicillin and streptomycin) ...
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bioRxiv - Cancer Biology 2019Quote: ... with 10% Fetal Calf Serum (FCS) (Sigma-Aldrich, St Louis, MO, USA) and 2 mM L-Glutamine (BE17-605F ...
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bioRxiv - Cancer Biology 2020Quote: ... All media were supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich) and all cells were kept at 37°C with 5% CO2 ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... containing 7 % fetal calf serum (FCS) (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 1% GlutaMAX (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... + 10% fetal calf serum (FCS; Sigma Aldrich, St. Louis, Missouri, United States) on plastic or in PRIME-XV MSC Expansion XSFM medium (FUJIFILM Irvine Scientific ...