Labshake search
Citations for Millipore Sigma :
1101 - 1150 of 2557 citations for CEP57 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... The sample was eluted with 100 μg/ml of 3X FLAG peptide (Sigma-Aldrich) in buffer containing 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples were eluted with 200 μg/ml of 3xFLAG peptide (Millipore Sigma cat #F4799) in TBS supplemented with 10 mM CaCl2 ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.5 mg of lyophilized CTD peptides were dissolved in 30 µl DMSO (Sigma-Aldrich). One molar equivalent of Atto-488 NHS ester (Atto-tec ...
-
bioRxiv - Molecular Biology 2022Quote: ... washed in Lysis buffer with 225mM salt and Eluted with 3X Flag peptide (Sigma). The eluted fraction was loaded on 4-15% gradient gels with Input and immunoblotted with appropriate antibodies.
-
bioRxiv - Neuroscience 2019Quote: ... The peptides were eluted into collection tubes and acidified with formic acid (Sigma Aldrich). Afterwards ...
-
bioRxiv - Plant Biology 2021Quote: ... The peptide samples were desalted and purified with C18 ZipTip (Millipore ZipTips, micro-C18). The 1.0 μl of each sample was loaded on MALDI plate with 1.0 μl of the matrix ...
-
bioRxiv - Molecular Biology 2020Quote: ... or from anti-Flag resin with buffer containing 250 μg/ml 3xFlag peptide (Sigma). Fractions containing protein were pooled and loaded on a Superdex 200 column preequilibrated in Amylose/Flag buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The peptides are acidified with 10 μl 10 % trifluoroacetic acid (TFA, Sigma-Aldrich, T6508). One Evotip (Evosep ...
-
bioRxiv - Molecular Biology 2022Quote: ... The synthesized peptides were dissolved in ultrapure water (Milli-Q system, Millipore, Bedford, MA), store at 4°C for later use.
-
bioRxiv - Microbiology 2022Quote: ... The resulting peptides were desalted using C18 ZipTip (Catalog No. ZTC18S096, Millipore, Boston, MA) as indicated previously described [22] ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were eluted from pooled beads with 0.5mg/ml FLAG peptide (#F3290, Millipore Sigma) by shaking at 1000 rpm for 1 hour at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... and immunoprecipitated proteins were eluted 3 times with 150µL 3x-FLAG peptides (Sigma, F4799) for 25 min each at 25°C ...
-
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 50 mM biotin were replaced with 200 μg/mL 3x FLAG peptide (Sigma-Aldrich). Samples were buffer-exchanged into wash buffer containing DTT (dilution factor 1:400 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted with Wash Buffer containing 0.2 mg/ml 3xFLAG peptide (Sigma). CSB proteins were further purified by gel filtration (Superdex 200 Increase ...
-
bioRxiv - Cell Biology 2019Quote: ... the beads were eluted in 30µl 300 µg/ml 3XFLAG peptide (Sigma-Aldrich, #F4799) at 4°C for 45mins ...
-
bioRxiv - Developmental Biology 2019Quote: ... A standard set of phosphopeptides (4 pmol/peptide sample, MS PhosphoMix1 Light, Sigma, MSPL1) were spiked into each sample ...
-
bioRxiv - Immunology 2021Quote: ... pulsed or not with 1 or 10 μg/ml OVA peptide 323–339 (Sigma) for 30 min at 37°C ...
-
bioRxiv - Cell Biology 2019Quote: ... beads were incubated with 200 μg/ml 3x FLAG peptides (Sigma-Aldrich, F4799-4MG) for 2 hrs at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... The reduced peptide was alkylated with 35 μL of 1.5 M iodoacetamide (Sigma-Aldrich) and incubated in the dark at room temperature for 15 minutes ...
-
bioRxiv - Cell Biology 2019Quote: ... Complexes were eluted with buffer #2 supplemented with 150 ug/ml 3xFLAG peptide (Sigma) for 1 hr at 4°C ...
-
bioRxiv - Bioengineering 2021Quote: ... peptides were dialyzed with 500 - 1200 MWCO dialysis tubing (Sigma-Aldrich, St. Louis, MO) against DI water ...
-
bioRxiv - Bioengineering 2021Quote: ... The crude peptides were the precipitated in ice cold diethyl ether (Et2O; Sigma-Aldrich), collected by centrifugation ...
-
bioRxiv - Cell Biology 2021Quote: ... the peptides were purified and desalted via ZipTip C18 columns (Millipore, Burlington, MA, USA). The digests were analyzed via nanoLC-MS/MS in a Thermo Scientific Q-Exactive Mass Spectrometer coupled to a nanoHPLC EASY-nLC 1000 (Thermo Scientific) ...
-
bioRxiv - Immunology 2020Quote: ... Peptide was stored at 10 mg/ml in 100% dimethyl sulfoxide (DMSO; Sigma-Aldrich) at −20°C.
-
bioRxiv - Molecular Biology 2021Quote: Primary rabbit antibodies used in this study were anti-calmodulin binding peptide (CBP; Millipore), anti-Glucose-6-phosphate dehydrogenase (G6PDH ...
-
bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used were rabbit anti-calcitonin gene-related peptide (1:5000, Sigma C8198) and rabbit anti-protein gene product 9.5 (1:500 ...
-
bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were activated in vitro with 2 μg of SIINFEKL peptides (Sigma) in the presence of recombinant human IL-2 (50 U/ml ...
-
bioRxiv - Biochemistry 2022Quote: ... then eluted in 100 mM elution buffer + 1 mg/ml 3× Flag peptide (Sigma) at 4°C for 30 min.
-
bioRxiv - Neuroscience 2022Quote: ... lyophilized peptides were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Cat# 52517, Millipore Sigma) to ensure that the starting material was in a homogenous ...
-
bioRxiv - Physiology 2023Quote: ... Samples containing 4 μg of peptides were de-salted using C18 Zip-tips (Millipore) and resuspended in 20 μl of 2.5 % (v/v ...
-
bioRxiv - Immunology 2022Quote: Antigen mixture for immunization was prepared by mixing OVA(323–339) peptide (Sigma-Aldrich) or NP-OVAL (Ovalbumin ...
-
bioRxiv - Neuroscience 2022Quote: ... the complex was eluted using 0.15 mg/ml 3×FLAG peptide (Millipore Cat# F4799) in GDN buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... The resulting peptide mixture was desalted using C18-Zip tips (EMD Millipore, Billerica, MA), speed-vacuumed to dryness and suspended in 0.1% formic acid for injection into ...
-
bioRxiv - Systems Biology 2024Quote: ... Samples containing 4 µg of peptides were de-salted using C18 Zip-tips (Millipore) and resuspended in 20 µl of 2.5 % (v/v ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were eluted in 45 µL of FLAG peptide (300 µg/mL, Millipore Sigma) in Wash Buffer plus 0.05% RapiGest (Waters Corp ...
-
bioRxiv - Molecular Biology 2024Quote: ... Peptides were resuspended in MS buffer containing 2% ACN in 1% Formic Acid (Sigma).
-
bioRxiv - Microbiology 2024Quote: ... Bound protein was eluted with 100 µL of 1.5 mg/mL FLAG peptide (Millipore) resuspended in wash buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... Peptides were identified by indirect immunofluorescence using an anti-myc antibody (C3956, Sigma-Aldrich). SW13-human adrenocarcinoma vimentin-deficient cells ...
-
bioRxiv - Biochemistry 2024Quote: ... The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen) were amplified with PCR using Q5 polymerase (New England Biolabs ...
-
bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
-
bioRxiv - Biochemistry 2023Quote: ... 15 µg of peptide was incubated with 50 µl of 33 mM iodoacetamide (Sigma) in degassed 5% formic acid (pH 4 ...
-
bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 10 µL 5% DMSO in LC-MS water (Sigma Aldrich) in an ultrasonic bath for 10 min.
-
bioRxiv - Neuroscience 2023Quote: ... The precipitated sample were eluted with 100 μg/ml 3XFLAG peptide (Sigma-Aldrich, F4799). The eluted samples were split in half for reducing (mix with 5x SDS sample buffer at 100°C for 10 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted in two column volumes of 0.2 mg/ml 3xFLAG peptide (Sigma) and dialyzed against 50 mM HEPES pH 7.4 ...
-
bioRxiv - Developmental Biology 2023Quote: ... 10 µg of peptides were further desalted with C18 ZipTips (Merck Millipore, Darmstadt, Germany) to ensure complete removal of beads.
-
bioRxiv - Physiology 2023Quote: ... peptides were dissolved in 18:1:1 (by volume) of saline:Kolliphor EL (Sigma Aldrich):DMSO and administered to mice by intraperitoneal injections at a volume of 10 µl/g at the indicated doses for the indicated times ...
-
bioRxiv - Molecular Biology 2023Quote: ... VCF1 C-terminal peptide stocks (150 μM) were prepared in pure DMSO (Sigma Aldrich). Threefold serial dilutions of VCF1 proteins or peptides were prepared in the SPR running buffer from the primary stocks starting from highest concentration of 300 nM (VCF1 C-terminal peptides ...
-
bioRxiv - Physiology 2023Quote: ... Peptides from each of these fractions were cleaned up using C18 ZipTip (Millipore, USA). Each fraction was then analysed on a quadrupole-TOF hybrid mass spectrometer (TripleTOF 6600 ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were eluted using 10 mM glutathione or 0.5 mg/mL 3XFLAG peptide (Sigma) in Lysis Buffer with 2 mM DDM.