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Citations for Millipore Sigma :
1101 - 1150 of 1195 citations for Atosiban Acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Macrophages were generated by culturing THP-1 (6×105/ml) cells with 15 nM phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) in the media ...
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bioRxiv - Immunology 2022Quote: ... cells were first restimulated in vitro with 50ng/mL phorbol 12-myristate 13-acetate (PMA) + 2 μg/mL ionomycin (both Sigma-Aldrich) in the presence of Monensin (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Both ex vivo and IL-25-stimulated cells were cultured in complete RPMI medium in the presence of phorbol-12-myristate-13-acetate (500 ng/ml) (Sigma-Aldrich) and ionomycin (250 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... One well per NHP was stimulated with 10 pg/mL phorbol myristate acetate (PMA) and 1 μg/mL ionomycin (Sigma-Aldrich) (P+I ...
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bioRxiv - Cell Biology 2023Quote: THP1 cells were differentiated into macrophages by treating 1M cells /mL with 0.5 μM phorbol 12-myristate 13-acetate (PMA; Sigma; cat P8139) for 3 h on Day 0 ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were stained with 0.4% uranyl acetate for 10 min and lead stain solution (Sigma-Aldrich, St. Louis, MO, USA) for 2 min and coated with osmium tetroxide using an osmium coater (HPC-1SW ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using the PEG/Lithium acetate method (Yeast Transformation Kit, MilliporeSigma)(Gietz and Woods 2002) and plated on non-inducing selective synthetic media (Sigma-Aldrich® Yeast Synthetic Drop-out Media Supplements ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 monocytes were differentiated to macrophages with medium containing 50 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma #P1585) for 3 days ...
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bioRxiv - Immunology 2023Quote: ... and 10-12 were treated with 100 µL of embryo water plus 1 µg/mL H2DCFDA and 400 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich). The plates are covered with aluminum foil and incubated at 28°C for 2.5 hours ...
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bioRxiv - Immunology 2023Quote: ... 4°C for 5min prior to stimulation in complete RPMI-1640 supplemented with 50ng/ml phorbol 12-myristate 12-acetate (PMA; Sigma-Aldrich), 1µg/ml ionomycin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 5 × 105 cells/mL were seeded in the wells of flat-bottom tissue culture plates and matured into macrophages with 20 nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) during 72 h ...
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bioRxiv - Microbiology 2023Quote: Cells were treated as indicated in the figures or figure legends with the following compounds: PMA (phorbol 12-myristate 13-acetate, Sigma Aldrich), Valproic acid (Sigma Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... Then the pellet was resuspended in 1 mL 100 mM sodium acetate (pH 4.5) supplemented with amyloglucosidase powder (Sigma-Aldrich, 10115) to a final concentration of 35 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... THP-1 cells were plated to 24-well plate at a concentration of 2 × 105 viable cells per well and incubated for 48 h in 1 ml/well of media supplemented with 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for differentiation into macrophages ...
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bioRxiv - Systems Biology 2024Quote: ... the decidualization were induced by adding the following reagents into cell medium: Medroxyprogesterone 17-acetate (Sigma, M1629; final conc. 1.0 µM), E2 (estradiol ...
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bioRxiv - Molecular Biology 2024Quote: ... THP-1 monocyte differentiation was initiated by incubating the cells with 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma Aldrich) for 2 days at 37°C in a 5% CO2 environment ...
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bioRxiv - Microbiology 2024Quote: ... collected on carbon coated copper grids (EM Resolutions, UK), and stained sequentially with 2 % aqueous uranyl acetate (BDH, UK) and 0.5 % aqueous lead citrate (Sigma-Aldrich, UK). Sections were examined and imaged in an FEI Talos F200C TEM at 200 kV.
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bioRxiv - Microbiology 2024Quote: For the leukopenic model mice were immunosuppressed with 150 mg/kg of cyclophosphamide (Baxter) on days −3 and −1 plus 1 subcutaneous injection of 250 mg/kg hydrocortisone 21-Acetate (Sigma-Aldrich) on day −1 ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... Host cell lines included U937 monocytes (ATCC CRL-1593.2) which were differentiated into macrophages with 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) and A ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... cells were plated on 24-well plates at 3x105 cells/well with 0.1 μg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) to promote differentiation ...
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bioRxiv - Cell Biology 2024Quote: ... 106 THP-1 cells were transferred to each of the six-well plates and cultured with 100ng/mL phorbol 12-myristate 13-acetate (PMA) (P8139, Sigma-Aldrich) for 24h ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell pellets were suspended in 500 µl of IP buffer (20 mM HEPES-KOH, pH 7.6, 125 mM potassium acetate, 1 mM EDTA, 20% glycerol, 1mM DTT, 1% NP-40, 1x Sigma protease inhibitor cocktail). One ml of acid-washed glass beads was added to each tube and the cells were lysed by bead beating for 8 minutes with incubation on ice for 3 minutes after each minute ...
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bioRxiv - Genomics 2020Quote: ... cells were grown to a density of 8.0 × 105 cells/mL and treated with 25ng/mL Phorbol 12-Myristate 13-Acetate (PMA) (Sigma-Aldrich, Catalogue #P8139) for 4 days ...
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bioRxiv - Microbiology 2021Quote: ... The degraded products of the dye in the filtrate were extracted with an equal volume of ethyl acetate (Sigma Aldrich; HPLC grade) and dried over sodium sulphate (Na2SO4) ...
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bioRxiv - Genomics 2021Quote: ... The tissue was mechanically lysed with a scalpel in a total volume of 5 ml lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Biochemistry 2020Quote: ... methanol (LC-MS grade), 2,5-dihydroxyacetophenone (DHA, ≥99.5%, Ultra pure), and anhydrous sodium acetate (NaOAc, ≥99%) were all purchased from Sigma Aldrich (Zwijndrecht, The Netherlands) and used without further purification.
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bioRxiv - Neuroscience 2021Quote: ... Baseline fluorescence was recorded for 30s before application of either vehicle or 1μM phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich, Cat# P8139) and imaged every 10s for 5 minutes at 50% laser power ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Systems Biology 2020Quote: ... For macrophage differentiation U937 cells were suspended in medium with 20 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, #P1585) and plated in 96-well microplates with µClear®flat bottom (Greiner ...
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bioRxiv - Cancer Biology 2020Quote: HL60 cells were plated at a density of 4×105 cells/ml and differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA) (Sigma, Darmstadt, Germany) at a final concentration of 32 nM for 6 days ...
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bioRxiv - Cancer Biology 2020Quote: ... It was mechanically dissociated using a scalpel in 1 ml of lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were re-suspended in 5 ml washing buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Microbiology 2022Quote: ... and macrophage-like differentiation was induced upon a twenty-four hours treatment with 100ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma cat. P1585). Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat ...
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bioRxiv - Molecular Biology 2019Quote: ... Thirty to forty-five micrograms of the protein were separated on a 5-12% Tris-acetate gel and electrophoretically transferred to PVDF membranes (Millipore, Billerica, MA). Membranes where then incubated with the primary antibodies against sXbp1 (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... cell suspensions in RPMI medium supplemented with 10% FBS were re-stimulated for 4 hr with phorbol 12-myristate 13-acetate (PMA, Sigma, 50ng/mL) and ionomycin (Sigma ...
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bioRxiv - Microbiology 2022Quote: wtKSHV inoculum was obtained from BCBL-1 cells (5×105 cells/ml) induced with 20 ng of TPA (12-O-tetradecanoylphorbol-13-acetate; Sigma, St.Louis, USA)/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were seeded at a density of 1x105 cells/ml in 96 well plates and differentiated for 48 hours with 25 nm PMA (phorbol-12-myristate-13-acetate, Sigma-Aldrich, #P1585). Cells were then left to rest for 24 hours in fresh media containing no PMA prior to bleomycin exposures.
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well cell culture-treated plates and differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 72 h in serum-free RPMI 1640 medium (SFM ...
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bioRxiv - Plant Biology 2022Quote: ... Relative quantification of all the VOCs was done by normalizing the peak area of the analytes with that of the internal standard nonyl acetate (Sigma-Aldrich, India).
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Microbiology 2023Quote: ... Jurkat Timer clones were treated for 24 h with a combination of T-cell stimulants: phorbol 12-myristate 13-acetate (SIGMA, Cat# P1585) at a final concentration of 20 ng/ml and ionomycin (Nacalai Tesque ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated in complete RPMI media containing 200 ng/mL of Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, #P8139) for 48 hours ...
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bioRxiv - Genomics 2022Quote: ... The total culture was then extracted through rigorous shaking with an equal volume of ethyl acetate (Sigma-Aldrich, St. Louis, MO, USA). The organic layer was removed and dried over anhydrous MgSO4 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... TC-HSMAM1 and Cas9-TC-HSMAM1 cells were maintained in a complete RPMI-1640 medium and 100 ng/mL of phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich, USA) at 37°C for 48 h to induce cell differentiation to macrophages 31 ...
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bioRxiv - Microbiology 2024Quote: ... cell differentiation into macrophages was induced by adding 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) to the medium for 72 hours.
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bioRxiv - Microbiology 2023Quote: ... Corresponding water samples were taken by filtering 2-L of tank water through 0.22-μm pore-size cellulose acetate membranes (Millipore Sigma, Burlington, MA). Samples were stored at −80°C until processing.
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bioRxiv - Molecular Biology 2024Quote: ... VFLIP for narrow window selection was buffer exchanged into 200 mM ammonium acetate using 50 kDa molecular weight cut-off (MWCO) Amicon® Ultra Centrifugal Filter (Sigma Aldrich). Samples were run at approximately 1 µM complex concentration for native mass spectrometry ...
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bioRxiv - Microbiology 2020Quote: Proteolysis was performed using 2 μM of encapsulated or unencapsulated DypB in proteolysis buffer (100mM Sodium acetate pH 5, 2mM DTT) with or without the addition of 1 μM Cathepsin B (Sigma-Aldrich Cat# 219362). Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from the throat swabs collections stored as frozen PBS suspensions at −20°C using a manual TRI Reagent – chloroform extraction and sodium acetate – ethanol precipitation (Sigma-Aldrich, Poznań, Poland). Presence of SARS-CoV-2 material in the collected sample was tested using GeneFinder real-time COVID-19 plus kit (OSANG Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... medium, containing 3% sucrose, 10−6 M potassium indole acetate (Nacalai Tesque, Kyoto, Japan) and 10−5 M kinetin (Sigma, St. Louis, MO) at 25 °C ...