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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 2x 10 mL PBS and replaced with 20 mL glucose-free DMEM + 10% dialyzed FBS + 50 µM D-biotin (Sigma-Aldrich B4639). 16 mM D-glucose was added to control plates ...
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bioRxiv - Cancer Biology 2020Quote: ... with the exchange buffer [0.5% SDS in 50 mM NH4HCO3 (09830, Sigma-Aldrich) aqueous solution] ...
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bioRxiv - Biochemistry 2021Quote: ... protein was then loaded on to Fractogel EMD SO3- cation exchange column (Millipore) in 20 mM MES ...
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bioRxiv - Immunology 2023Quote: ... cation exchange chromatography using Fractogel® EMD SO3-(S) resin (Merck Millipore, US) for intermediated purification ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.4 and then by buffer exchange using a 10kDa concentrator (Amicon, Millipore). The concentration of the labelled protein was estimated by measuring the absorbance at 495 nm using a molar extinction coefficient of 77,000 M-1cm-1 and the total protein concentration was measured at 280 nm ...
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bioRxiv - Biophysics 2024Quote: ... Pooled fractions were then loaded onto a MonoQ ion-exchange column (Sigma-Aldrich) and eluted across a salt gradient of 0.5 M NaCl ...
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bioRxiv - Immunology 2023Quote: ... and subsequently loaded into ion exchange columns containing DOWEX 1X2-400 resin (Sigma). FAO product was eluted with water and radiation was measured by liquid scintillation.
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bioRxiv - Cancer Biology 2021Quote: 0.08 mg of Cy5.5 in 100 μL water was mixed with 0.2 mg of PLL in 1 mM 100 μL NaHCO3 in the dark at room temperature for 30 min (vortexed every 10 min) and filtered through molecular-weight cutoff membrane filters (10 kDa, Millipore). The resulting PLL-Cy5.5 was collected and washed several times with sterilized water until the color of the filtrate was clear ...
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bioRxiv - Cell Biology 2023Quote: ... 10-30μg total protein was loaded and separated by 10% SDS-PAGE gel and transferred to PVDF membranes (Millipore; GVHP29325). Then ...
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bioRxiv - Microbiology 2022Quote: ... The samples were boiled for 10 min and separated in a 10% SDS-PAGE gel and transferred to 0.45 μm nitrocellulose membrane (Millipore, USA). For detection of S protein ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30μg total protein was loaded and separated by 10% SDS-PAGE gel and transferred to PVDF membranes (Millipore; GVHP29325). Then ...
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bioRxiv - Immunology 2024Quote: Cells from draining popliteal lymph nodes (1 x 106) were stimulated for 4 h at 37 °C with PMA (phorbol 12-myristate 13-acetate, 10 ng/mL, Sigma-Aldrich, Darmstadt, He, Germany) and Ionomycin (10 ng/mL ...
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bioRxiv - Molecular Biology 2022Quote: For qualitative determination of superoxide anion release from mycelia a histochemical nitro blue tetrazolium (NBT, Sigma-Aldrich, N6876) staining was performed as previously described (Warnsmann et al. ...
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bioRxiv - Microbiology 2020Quote: ... for which a cellulose membrane (10 kDa) was used in the Amicon Ultracel (Millipore) ultrafiltration system at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... PVDF membranes were blocked in 5% milk/TBS 10% glycerol + 0.05% tween20 (Sigma-Aldrich) (TBS/t ...
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bioRxiv - Neuroscience 2021Quote: ... PVDF membranes were submerged for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich) in 40% ethanol and 10% acetic acid ...
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bioRxiv - Molecular Biology 2020Quote: ... separated in 10% SDS-polyacrylamide gel and transferred on Immobilon-P membrane (Millipore #IPVH00010).
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bioRxiv - Plant Biology 2021Quote: ... The membrane was developed by adding 10 ml of BCIP/NBT (Sigma Aldrich, USA) solution to the membrane and incubating for 20 min.
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bioRxiv - Cell Biology 2020Quote: ... loaded onto 10% SDS-polyacrylamide gels and subsequently transferred onto nitrocellulose membranes (Protran, Sigma). Membranes were blocked with Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% or 12 % SDS-polyacrylamide gel and transferred to a polyvinylidinedifluoride membrane (PVDF, Millipore). Primary antibodies were added and the membranes were incubated overnight at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were concentrated by centrifugation with a 10 kDa cut-off membrane (Merck Millipore) to a volume of 300 µl ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred onto PVDF membranes (Millipore). After blocked in 5% skim milk for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated in primary antibodies mouse anti-Vinculin (Sigma, V9264, 1:10 000), mouse anti-beta-catenin (BD 610154 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were resolved in 10% SDS/PAGE and transferred to nitrocellulose membranes (Sigma Aldrich). Membranes were probed for 2 h at RT (or overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 % acrylamide gels and transferred to a 0.45 μm Nitrocellulose membrane (Merck Millipore). After blocking with 5 % milk in TBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... separated by 10% SDS-PAGE and transferred onto a polyvinylidene difluoride membrane (Millipore, USA). After blocking with 5% skimmed milk ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were separated on 10% SDS‒PAGE gels and transferred onto PVDF membranes (Millipore). Membranes were blocked with 5% milk or 3% BSA for 1 hr and incubated with specific antibodies overnight at 4 °C on a shaker ...
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bioRxiv - Neuroscience 2024Quote: ... PVDF membranes were saturated for 10 minutes with 0.0008% Direct Blue 71 (Sigma-Aldrich) in 40% ethanol and 10% acetic acid ...
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bioRxiv - Microbiology 2021Quote: ... groundwater was pumped directly into a sterile chilled Nalgene filtration unit fitted with a 0.22 µm pore size Isopore polycarbonate membrane (Merck Millipore, Darmstadt, Germany). Approximately 10 L of groundwater was filtered each for metagenomic and metaproteomic sample and 1 L was filtered when samples were taken for 16S rRNA gene amplicon analysis only ...
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bioRxiv - Microbiology 2020Quote: ... to obtain a final concentration of 80 % acetone (v/v) and pipetted on an Ultrafree-MC PTFE membrane 0.2 µm filter (Merck Millipore, Burlington, MA). The filter was incubated for 2 min at room temperature and was then centrifuged at 5000 x g for 1 min ...
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bioRxiv - Neuroscience 2021Quote: The edges of whole retinas were nicked to allow the retina to lay flat and placed on a nitrocellulose membrane (0.8 µm, Millipore, MA, USA) with the photoreceptor side up ...
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bioRxiv - Immunology 2022Quote: ... Antibody preparations for in vivo infusions were micro-filtered (Ultrafree®-CL devices - 0.1 µm PVDF membrane, Merck-Millipore, Darmstadt, Germany), and checked for endotoxins levels using the ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (GenScript).
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bioRxiv - Plant Biology 2022Quote: ... 250 ml log-phase culture cells were removed by centrifugation and the supernatant filtered through 0.22 µm membranes (Millipore, Billerica, MA). Solid phase extractions of the supernatants were done using benzene sulphonic acid cation exchanger on silica (Macherey Nagel ...
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bioRxiv - Microbiology 2021Quote: ... DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore, Cork, Ireland) and DNA using the DNeasy PowerLyzer PowerSoil DNA extraction kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was cut into 410 µM thick sections on a tissue chopper and cultured at the medium/air interface on membranes (Millipore PICMORG50) at 37° C in 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... The water samples were transferred to the lab in a cooling box where upon arrival 30 ml water from the turbid soda pans and 50 ml from the brown pan were filtered onto 0.1 µm pore size filters (MF-Millipore membrane filter) to collect planktonic microbial cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
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bioRxiv - Physiology 2023Quote: ... The pelleted cells of the cardiac myocyte fraction were resuspended in ice-cold 7 mL Perfusion Buffer and applied onto a 20-µm nylon net membrane (Millipore, SCNY00020). Trapped cells on the membrane were collected with 7 mL Perfusion Buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred by wet blot transfer on 0.2 µM pore size PVDF membrane (Amersham, GE10600021) and immunoblotted using MCU (Sigma-Aldrich, HPA016480) according to the standard protocol.
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bioRxiv - Immunology 2024Quote: ... was added to a well of a 24 well plate and a 6.5 mm Transwell® Polycarbonate Membrane insert with a 5.0 µm pore (Costar #3421/Sigma # CLS3421-48EA) was added to each well ...
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bioRxiv - Microbiology 2024Quote: ... a volume of 20 mL with an OD720 of 0.6 was withdrawn from the culture grown in the desired conditions and vacuum filtered through a Durapore® 0.45 µm PVDF membrane (Merck Millipore-Ireland). The filter with the biomass was immediately inverted into a petri dish with 1.6 ml of pre-cooled methanol for quenching and incubated at −80°C for an hour with periodic rubbing of the filter against the container to aid cell lysis ...
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bioRxiv - Microbiology 2024Quote: ... for 10 minutes in the dark and then filtered through Nuclepore black polycarbonate membrane filters (0.22 µm pore size, Merck Millipore, Darmstadt, Germany) with negative pressure (≤ 20 kPa) ...
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bioRxiv - Microbiology 2023Quote: ... from Barcelona (Spain) was filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts) to remove bacteria ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the phage suspensions were further filtered through low protein binding 0.22 µm pore size polyethersulfone (PES) membrane filters (Millex-GP, Millipore, Bedford, Massachusetts), diluted and plated as indicated in the previous paragraph to verify the uniformity of the plaques ...
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bioRxiv - Neuroscience 2024Quote: ... 400 µm slices were cut with a vibratome and transferred to a membrane culture insert (Sigma-Aldrich, PICMORG50, St. Gallen, Switzerland). The culture media consisted of 50% MEM (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... depending on the molecular weight of the target protein and transferred to 0.45 µm Immobilon®-FL PVDF Membrane (Millipore, IPFL00010). The membranes were blocked with blocking solution (5% w/v milk in Tris-buffered saline ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were equilibrated in pre-chilled transfer buffer with 15% methanol and then protein was transferred to a 0.45 µm nitrocellulose membrane (Sigma catalog# 10600002) via wet electroblotting on ice for 1 h at 300 mV ...
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bioRxiv - Microbiology 2024Quote: ... swab samples in VTM were centrifuged at 3200 RPM for 15 minutes and the resulting liquid was passed through a 0.22 µm filter (Membrane Solutions, USA) and then the filtrate was treated with a mix of nucleases: Benzonase (Sigma-Aldrich, USA), Turbo DNAse ...
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bioRxiv - Cell Biology 2024Quote: ... The delipidated fetal bovine serum (dFBS) was sterilized by filtration with a 0.22 µm membrane (Millex-GV, Millipore S.A., Molsheim, France).
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bioRxiv - Immunology 2021Quote: ... cells were treated with 10 µM of the NLRP3 inflammasome inhibitor MCC950 (CP-456773, Sigma-Aldrich) after LPS priming and during inflammasome activation.